N-Acetylcysteine Suppresses LPS-Induced Pathological Angiogenesis
Background/Aims: Angiogenesis is a key feature during embryo development but is also part of the pathogenesis of cancer in adult life. Angiogenesis might be modulated by inflammation. Methods: We established an angiogenesis model in chick chorioallantoic membrane (CAM) induced by the exposure of lip...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2018-09-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | https://www.karger.com/Article/FullText/493874 |
| _version_ | 1819044070361661440 |
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| author | Ping Zhang Shan Zhong Guang Wang Shi-yao Zhang Chang Chu Shufei Zeng Yu Yan Xin Cheng Yongping Bao Berthold Hocher Xuesong Yang |
| author_facet | Ping Zhang Shan Zhong Guang Wang Shi-yao Zhang Chang Chu Shufei Zeng Yu Yan Xin Cheng Yongping Bao Berthold Hocher Xuesong Yang |
| author_sort | Ping Zhang |
| collection | DOAJ |
| description | Background/Aims: Angiogenesis is a key feature during embryo development but is also part of the pathogenesis of cancer in adult life. Angiogenesis might be modulated by inflammation. Methods: We established an angiogenesis model in chick chorioallantoic membrane (CAM) induced by the exposure of lipopolysaccharide (LPS), and analyzed the effects of the antioxidant N-acetylcysteine (NAC) on angiogenesis in this model as well as on the expression of key genes known to involved in the regulation of angiogenesis. Results: Treatment with NAC was able to normalize LPS induced angiogenesis and restore the LPS-induced damage of vascular epithelium in chick CAM. Using quantitative PCR, we showed that NAC administration normalized the LPS induced expression of Keap1-Nrf2 signaling and oxidative stress key enzyme gene expressions (SOD, GPx and YAP1). Conclusion: We established a LPS-induced angiogenesis model in chick CAM. NAC administration could effectively inhibit LPS-induced angiogenesis and restore the integrity of endothelium on chick CAM. LPS exposure caused an increased expression of genes involved in oxidative stress in chick CAM. NAC administration could abolish this effect. |
| first_indexed | 2024-12-21T10:06:50Z |
| format | Article |
| id | doaj.art-2d434253f5b648a19809a67e45602921 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-21T10:06:50Z |
| publishDate | 2018-09-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-2d434253f5b648a19809a67e456029212022-12-21T19:07:48ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782018-09-014962483249510.1159/000493874493874N-Acetylcysteine Suppresses LPS-Induced Pathological AngiogenesisPing ZhangShan ZhongGuang WangShi-yao ZhangChang ChuShufei ZengYu YanXin ChengYongping BaoBerthold HocherXuesong YangBackground/Aims: Angiogenesis is a key feature during embryo development but is also part of the pathogenesis of cancer in adult life. Angiogenesis might be modulated by inflammation. Methods: We established an angiogenesis model in chick chorioallantoic membrane (CAM) induced by the exposure of lipopolysaccharide (LPS), and analyzed the effects of the antioxidant N-acetylcysteine (NAC) on angiogenesis in this model as well as on the expression of key genes known to involved in the regulation of angiogenesis. Results: Treatment with NAC was able to normalize LPS induced angiogenesis and restore the LPS-induced damage of vascular epithelium in chick CAM. Using quantitative PCR, we showed that NAC administration normalized the LPS induced expression of Keap1-Nrf2 signaling and oxidative stress key enzyme gene expressions (SOD, GPx and YAP1). Conclusion: We established a LPS-induced angiogenesis model in chick CAM. NAC administration could effectively inhibit LPS-induced angiogenesis and restore the integrity of endothelium on chick CAM. LPS exposure caused an increased expression of genes involved in oxidative stress in chick CAM. NAC administration could abolish this effect.https://www.karger.com/Article/FullText/493874N-acetylcysteineNACLipopolysaccharideLPSPathological angiogenesisChick CAMOxidative stress |
| spellingShingle | Ping Zhang Shan Zhong Guang Wang Shi-yao Zhang Chang Chu Shufei Zeng Yu Yan Xin Cheng Yongping Bao Berthold Hocher Xuesong Yang N-Acetylcysteine Suppresses LPS-Induced Pathological Angiogenesis Cellular Physiology and Biochemistry N-acetylcysteine NAC Lipopolysaccharide LPS Pathological angiogenesis Chick CAM Oxidative stress |
| title | N-Acetylcysteine Suppresses LPS-Induced Pathological Angiogenesis |
| title_full | N-Acetylcysteine Suppresses LPS-Induced Pathological Angiogenesis |
| title_fullStr | N-Acetylcysteine Suppresses LPS-Induced Pathological Angiogenesis |
| title_full_unstemmed | N-Acetylcysteine Suppresses LPS-Induced Pathological Angiogenesis |
| title_short | N-Acetylcysteine Suppresses LPS-Induced Pathological Angiogenesis |
| title_sort | n acetylcysteine suppresses lps induced pathological angiogenesis |
| topic | N-acetylcysteine NAC Lipopolysaccharide LPS Pathological angiogenesis Chick CAM Oxidative stress |
| url | https://www.karger.com/Article/FullText/493874 |
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