Current Advances in Nanotechnology for the Next Generation of Sequencing (NGS)
This communication aims at discussing strategies based on developments from nanotechnology focused on the next generation of sequencing (NGS). In this regard, it should be noted that even in the advanced current situation of many techniques and methods accompanied with developments of technology, th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/2/260 |
| _version_ | 1797622050099036160 |
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| author | Angel Guillermo Bracamonte |
| author_facet | Angel Guillermo Bracamonte |
| author_sort | Angel Guillermo Bracamonte |
| collection | DOAJ |
| description | This communication aims at discussing strategies based on developments from nanotechnology focused on the next generation of sequencing (NGS). In this regard, it should be noted that even in the advanced current situation of many techniques and methods accompanied with developments of technology, there are still existing challenges and needs focused on real samples and low concentrations of genomic materials. The approaches discussed/described adopt spectroscopical techniques and new optical setups. PCR bases are introduced to understand the role of non-covalent interactions by discussing about Nobel prizes related to genomic material detection. The review also discusses colorimetric methods, polymeric transducers, fluorescence detection methods, enhanced plasmonic techniques such as metal-enhanced fluorescence (MEF), semiconductors, and developments in metamaterials. In addition, nano-optics, challenges linked to signal transductions, and how the limitations reported in each technique could be overcome are considered in real samples. Accordingly, this study shows developments where optical active nanoplatforms generate signal detection and transduction with enhanced performances and, in many cases, enhanced signaling from single double-stranded deoxyribonucleic acid (DNA) interactions. Future perspectives on miniaturized instrumentation, chips, and devices aimed at detecting genomic material are analyzed. However, the main concept in this report derives from gained insights into nanochemistry and nano-optics. Such concepts could be incorporated into other higher-sized substrates and experimental and optical setups. |
| first_indexed | 2024-03-11T09:04:47Z |
| format | Article |
| id | doaj.art-2d4387774ff24bf2ad305f890e9e7e71 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T09:04:47Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-2d4387774ff24bf2ad305f890e9e7e712023-11-16T19:26:23ZengMDPI AGBiosensors2079-63742023-02-0113226010.3390/bios13020260Current Advances in Nanotechnology for the Next Generation of Sequencing (NGS)Angel Guillermo Bracamonte0Instituto de Investigaciones en Físicoquímica de Córdoba (INFIQC), Departamento de Química Orgánica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, ArgentinaThis communication aims at discussing strategies based on developments from nanotechnology focused on the next generation of sequencing (NGS). In this regard, it should be noted that even in the advanced current situation of many techniques and methods accompanied with developments of technology, there are still existing challenges and needs focused on real samples and low concentrations of genomic materials. The approaches discussed/described adopt spectroscopical techniques and new optical setups. PCR bases are introduced to understand the role of non-covalent interactions by discussing about Nobel prizes related to genomic material detection. The review also discusses colorimetric methods, polymeric transducers, fluorescence detection methods, enhanced plasmonic techniques such as metal-enhanced fluorescence (MEF), semiconductors, and developments in metamaterials. In addition, nano-optics, challenges linked to signal transductions, and how the limitations reported in each technique could be overcome are considered in real samples. Accordingly, this study shows developments where optical active nanoplatforms generate signal detection and transduction with enhanced performances and, in many cases, enhanced signaling from single double-stranded deoxyribonucleic acid (DNA) interactions. Future perspectives on miniaturized instrumentation, chips, and devices aimed at detecting genomic material are analyzed. However, the main concept in this report derives from gained insights into nanochemistry and nano-optics. Such concepts could be incorporated into other higher-sized substrates and experimental and optical setups.https://www.mdpi.com/2079-6374/13/2/260next generation of sequencing (NGS)DNA detectiongenotypingenhanced strategiesnano-devicesnano-optics |
| spellingShingle | Angel Guillermo Bracamonte Current Advances in Nanotechnology for the Next Generation of Sequencing (NGS) Biosensors next generation of sequencing (NGS) DNA detection genotyping enhanced strategies nano-devices nano-optics |
| title | Current Advances in Nanotechnology for the Next Generation of Sequencing (NGS) |
| title_full | Current Advances in Nanotechnology for the Next Generation of Sequencing (NGS) |
| title_fullStr | Current Advances in Nanotechnology for the Next Generation of Sequencing (NGS) |
| title_full_unstemmed | Current Advances in Nanotechnology for the Next Generation of Sequencing (NGS) |
| title_short | Current Advances in Nanotechnology for the Next Generation of Sequencing (NGS) |
| title_sort | current advances in nanotechnology for the next generation of sequencing ngs |
| topic | next generation of sequencing (NGS) DNA detection genotyping enhanced strategies nano-devices nano-optics |
| url | https://www.mdpi.com/2079-6374/13/2/260 |
| work_keys_str_mv | AT angelguillermobracamonte currentadvancesinnanotechnologyforthenextgenerationofsequencingngs |