Phytase-Producing Bacteria from Extreme Regions in Indonesia
ABSTRACTIn this study, 154 isolates capable of producing extracellular phytate-degrading activity were isolated from four soil samples from volcanic areas in Central Java, Indonesia. Six strains with high phytate-degrading activity were selected for strain identification and characterization of the...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2015-10-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500711&lng=en&tlng=en |
| _version_ | 1818504204060196864 |
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| author | Sajidan Rita Wulandari Evy Novita Sari Adi Ratriyanto Hailu Weldekiros Ralf Greiner |
| author_facet | Sajidan Rita Wulandari Evy Novita Sari Adi Ratriyanto Hailu Weldekiros Ralf Greiner |
| author_sort | Sajidan |
| collection | DOAJ |
| description | ABSTRACTIn this study, 154 isolates capable of producing extracellular phytate-degrading activity were isolated from four soil samples from volcanic areas in Central Java, Indonesia. Six strains with high phytate-degrading activity were selected for strain identification and characterization of the corresponding phytate-degrading enzyme. Blast analysis of 16S rRNA gene sequences revealed high similarities for all the six isolates to reference sequences belonging to the genusBacillus. Isolates MS5, MC6, D10 and D16 showed 99% sequence identity toB. cereus, while isolate MC8 exhibited 99% sequence identity toB. aryabhatti and D6 99% sequence identity toB. psychrotolerans. The crude extracellular phytase preparations from the isolates showed following optimal conditions for phytate dephosphorylation: pH 4.0 and 50°C (isolate D10), pH 5.0 and 60°C (isolate MC6, and isolate MS5), pH 6.0 and 50°C (isolate D16) and pH 6.0 and 60°C (isolate D6) and pH 6.0 and 40°C (isolate MC8). Zn2+ and Fe3+ strongly inhibited phytate dephosphorylation with all phytase preparations studied. In the presence of Ca2+, an increase in phytase activity of 10-15% was obtained. |
| first_indexed | 2024-12-10T21:34:03Z |
| format | Article |
| id | doaj.art-2d45c1d2d17146b19d431f0965d8556c |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-10T21:34:03Z |
| publishDate | 2015-10-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-2d45c1d2d17146b19d431f0965d8556c2022-12-22T01:32:42ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242015-10-0158571171710.1590/S1516-89132015050173S1516-89132015000500711Phytase-Producing Bacteria from Extreme Regions in IndonesiaSajidanRita WulandariEvy Novita SariAdi RatriyantoHailu WeldekirosRalf GreinerABSTRACTIn this study, 154 isolates capable of producing extracellular phytate-degrading activity were isolated from four soil samples from volcanic areas in Central Java, Indonesia. Six strains with high phytate-degrading activity were selected for strain identification and characterization of the corresponding phytate-degrading enzyme. Blast analysis of 16S rRNA gene sequences revealed high similarities for all the six isolates to reference sequences belonging to the genusBacillus. Isolates MS5, MC6, D10 and D16 showed 99% sequence identity toB. cereus, while isolate MC8 exhibited 99% sequence identity toB. aryabhatti and D6 99% sequence identity toB. psychrotolerans. The crude extracellular phytase preparations from the isolates showed following optimal conditions for phytate dephosphorylation: pH 4.0 and 50°C (isolate D10), pH 5.0 and 60°C (isolate MC6, and isolate MS5), pH 6.0 and 50°C (isolate D16) and pH 6.0 and 60°C (isolate D6) and pH 6.0 and 40°C (isolate MC8). Zn2+ and Fe3+ strongly inhibited phytate dephosphorylation with all phytase preparations studied. In the presence of Ca2+, an increase in phytase activity of 10-15% was obtained.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500711&lng=en&tlng=en16S rDNA, Bacillus sp.bacterial phytasephytatephytate-degrading enzyme |
| spellingShingle | Sajidan Rita Wulandari Evy Novita Sari Adi Ratriyanto Hailu Weldekiros Ralf Greiner Phytase-Producing Bacteria from Extreme Regions in Indonesia Brazilian Archives of Biology and Technology 16S rDNA, Bacillus sp. bacterial phytase phytate phytate-degrading enzyme |
| title | Phytase-Producing Bacteria from Extreme Regions in Indonesia |
| title_full | Phytase-Producing Bacteria from Extreme Regions in Indonesia |
| title_fullStr | Phytase-Producing Bacteria from Extreme Regions in Indonesia |
| title_full_unstemmed | Phytase-Producing Bacteria from Extreme Regions in Indonesia |
| title_short | Phytase-Producing Bacteria from Extreme Regions in Indonesia |
| title_sort | phytase producing bacteria from extreme regions in indonesia |
| topic | 16S rDNA, Bacillus sp. bacterial phytase phytate phytate-degrading enzyme |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500711&lng=en&tlng=en |
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