Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa
Highlights 1. Verifying an entire native IPyA pathway of IAA synthesis in P. polymyxa. 2. Introducing heterologous IAM and IPyA pathways of IAA synthesis to P. polymyxa. 3. Selecting and analyzing a novel strong promoter P 04420 to express IAA synthesis genes.
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-08-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-022-02181-3 |
| _version_ | 1798042649734676480 |
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| author | Huimin Sun Jikun Zhang Wenteng Liu Wenhui E Xin Wang Hui Li Yanru Cui Dongying Zhao Kai Liu Binghai Du Yanqin Ding Chengqiang Wang |
| author_facet | Huimin Sun Jikun Zhang Wenteng Liu Wenhui E Xin Wang Hui Li Yanru Cui Dongying Zhao Kai Liu Binghai Du Yanqin Ding Chengqiang Wang |
| author_sort | Huimin Sun |
| collection | DOAJ |
| description | Highlights 1. Verifying an entire native IPyA pathway of IAA synthesis in P. polymyxa. 2. Introducing heterologous IAM and IPyA pathways of IAA synthesis to P. polymyxa. 3. Selecting and analyzing a novel strong promoter P 04420 to express IAA synthesis genes. |
| first_indexed | 2024-04-11T22:38:25Z |
| format | Article |
| id | doaj.art-2d4c3a58c36148da9b96e9b4b2e1fee3 |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-04-11T22:38:25Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-2d4c3a58c36148da9b96e9b4b2e1fee32022-12-22T03:59:06ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542022-08-0115111910.1186/s13068-022-02181-3Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxaHuimin Sun0Jikun Zhang1Wenteng Liu2Wenhui E3Xin Wang4Hui Li5Yanru Cui6Dongying Zhao7Kai Liu8Binghai Du9Yanqin Ding10Chengqiang Wang11College of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural UniversityCollege of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural UniversityCollege of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural UniversityCollege of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural UniversityCollege of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural UniversityCollege of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural UniversityCollege of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural UniversityCollege of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural UniversityCollege of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural UniversityCollege of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural UniversityCollege of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural UniversityCollege of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural UniversityHighlights 1. Verifying an entire native IPyA pathway of IAA synthesis in P. polymyxa. 2. Introducing heterologous IAM and IPyA pathways of IAA synthesis to P. polymyxa. 3. Selecting and analyzing a novel strong promoter P 04420 to express IAA synthesis genes.https://doi.org/10.1186/s13068-022-02181-3Paenibacillus polymyxaPGPRIAAMetabolic pathwayPromoter engineering |
| spellingShingle | Huimin Sun Jikun Zhang Wenteng Liu Wenhui E Xin Wang Hui Li Yanru Cui Dongying Zhao Kai Liu Binghai Du Yanqin Ding Chengqiang Wang Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa Biotechnology for Biofuels and Bioproducts Paenibacillus polymyxa PGPR IAA Metabolic pathway Promoter engineering |
| title | Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa |
| title_full | Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa |
| title_fullStr | Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa |
| title_full_unstemmed | Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa |
| title_short | Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa |
| title_sort | identification and combinatorial engineering of indole 3 acetic acid synthetic pathways in paenibacillus polymyxa |
| topic | Paenibacillus polymyxa PGPR IAA Metabolic pathway Promoter engineering |
| url | https://doi.org/10.1186/s13068-022-02181-3 |
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