Nematicidal Potential of Green Silver Nanoparticles Synthesized Using Aqueous Root Extract of <em>Glycyrrhiza glabra</em>
<i>Meloidogyne incognita</i> (root-knot nematode) is a devastating soil-borne pathogen which can infect almost all cultivated plants around the globe, expediting huge pecuniary losses. The purpose of current study was to use the aqueous root extract of <i>Glycyrrhiza glabra</i&g...
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MDPI AG
2022-08-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/17/2966 |
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| author | Kanika Rani Nisha Devi Prakash Banakar Pushpa Kharb Prashant Kaushik |
| author_facet | Kanika Rani Nisha Devi Prakash Banakar Pushpa Kharb Prashant Kaushik |
| author_sort | Kanika Rani |
| collection | DOAJ |
| description | <i>Meloidogyne incognita</i> (root-knot nematode) is a devastating soil-borne pathogen which can infect almost all cultivated plants around the globe, expediting huge pecuniary losses. The purpose of current study was to use the aqueous root extract of <i>Glycyrrhiza glabra</i> for synthesizing silver nanoparticles (GRAgNPs) and assess their nematicidal potential against <i>M. incognita</i> by in vitro methods, including hatching inhibition and mortality assays. The active uptake of FITC labeled GRAgNPs by the nematode and their effect on the expression of selected genes involved in oxidative stress and DNA damage repair were also studied. An HRTEM micrograph confirmed their spherical morphology with sizes ranging from 9.61 nm to 34.735 nm. Complete inhibition of egg-hatching was observed after 48 h of treatment with as low as 10.0 ppm of GRAgNPs. In addition, 100% mortality was recorded at the lowest dose of 6.0 ppm, after 12 h of treatment. The LC-50 for GRAgNPs was found to be 0.805 ± 0.177 ppm at <i>p</i> < 0.0001, R<sup>2</sup> = 0.9930, and α = 0.05. The expression of targeted genes (<i>skn-1</i>, <i>mev-1</i>, <i>sod-3</i>, <i>dhs-23</i>, <i>cyp-450</i>, <i>xpa</i>, <i>cpr-1</i>, <i>gst-n</i>, and <i>ugt</i>) was significantly enhanced (1.09–2.79 folds), at 1.0 ppm (α = 0.05, 95% CI) GRAgNPs treatment. In conclusion, GRAgNPs performed efficaciously and considerably in contrast to chemical nematicide and commercial silver nanoparticles (CAgNPs) and might be used as a promising alternative as relatively lower concentration and short exposure time were enough to cause higher mortality and nanotoxicity in nematodes. |
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| publishDate | 2022-08-01 |
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| spelling | doaj.art-2d507939ffa74183855190b35f0760822024-04-24T03:04:32ZengMDPI AGNanomaterials2079-49912022-08-011217296610.3390/nano12172966Nematicidal Potential of Green Silver Nanoparticles Synthesized Using Aqueous Root Extract of <em>Glycyrrhiza glabra</em>Kanika Rani0Nisha Devi1Prakash Banakar2Pushpa Kharb3Prashant Kaushik4Department of Molecular Biology, Biotechnology and Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, IndiaDepartment of Molecular Biology, Biotechnology and Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, IndiaDepartment of Nematology, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, IndiaDepartment of Molecular Biology, Biotechnology and Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, IndiaKikugawa Research Station, Yokohama Ueki, Kikugawa 439-0031, Japan<i>Meloidogyne incognita</i> (root-knot nematode) is a devastating soil-borne pathogen which can infect almost all cultivated plants around the globe, expediting huge pecuniary losses. The purpose of current study was to use the aqueous root extract of <i>Glycyrrhiza glabra</i> for synthesizing silver nanoparticles (GRAgNPs) and assess their nematicidal potential against <i>M. incognita</i> by in vitro methods, including hatching inhibition and mortality assays. The active uptake of FITC labeled GRAgNPs by the nematode and their effect on the expression of selected genes involved in oxidative stress and DNA damage repair were also studied. An HRTEM micrograph confirmed their spherical morphology with sizes ranging from 9.61 nm to 34.735 nm. Complete inhibition of egg-hatching was observed after 48 h of treatment with as low as 10.0 ppm of GRAgNPs. In addition, 100% mortality was recorded at the lowest dose of 6.0 ppm, after 12 h of treatment. The LC-50 for GRAgNPs was found to be 0.805 ± 0.177 ppm at <i>p</i> < 0.0001, R<sup>2</sup> = 0.9930, and α = 0.05. The expression of targeted genes (<i>skn-1</i>, <i>mev-1</i>, <i>sod-3</i>, <i>dhs-23</i>, <i>cyp-450</i>, <i>xpa</i>, <i>cpr-1</i>, <i>gst-n</i>, and <i>ugt</i>) was significantly enhanced (1.09–2.79 folds), at 1.0 ppm (α = 0.05, 95% CI) GRAgNPs treatment. In conclusion, GRAgNPs performed efficaciously and considerably in contrast to chemical nematicide and commercial silver nanoparticles (CAgNPs) and might be used as a promising alternative as relatively lower concentration and short exposure time were enough to cause higher mortality and nanotoxicity in nematodes.https://www.mdpi.com/2079-4991/12/17/2966<i>Meloidogyne incognita</i><i>Glycyrrhiza glabra</i>silver nanoparticlesnematicidal potentialnanotoxicitygreen synthesis |
| spellingShingle | Kanika Rani Nisha Devi Prakash Banakar Pushpa Kharb Prashant Kaushik Nematicidal Potential of Green Silver Nanoparticles Synthesized Using Aqueous Root Extract of <em>Glycyrrhiza glabra</em> Nanomaterials <i>Meloidogyne incognita</i> <i>Glycyrrhiza glabra</i> silver nanoparticles nematicidal potential nanotoxicity green synthesis |
| title | Nematicidal Potential of Green Silver Nanoparticles Synthesized Using Aqueous Root Extract of <em>Glycyrrhiza glabra</em> |
| title_full | Nematicidal Potential of Green Silver Nanoparticles Synthesized Using Aqueous Root Extract of <em>Glycyrrhiza glabra</em> |
| title_fullStr | Nematicidal Potential of Green Silver Nanoparticles Synthesized Using Aqueous Root Extract of <em>Glycyrrhiza glabra</em> |
| title_full_unstemmed | Nematicidal Potential of Green Silver Nanoparticles Synthesized Using Aqueous Root Extract of <em>Glycyrrhiza glabra</em> |
| title_short | Nematicidal Potential of Green Silver Nanoparticles Synthesized Using Aqueous Root Extract of <em>Glycyrrhiza glabra</em> |
| title_sort | nematicidal potential of green silver nanoparticles synthesized using aqueous root extract of em glycyrrhiza glabra em |
| topic | <i>Meloidogyne incognita</i> <i>Glycyrrhiza glabra</i> silver nanoparticles nematicidal potential nanotoxicity green synthesis |
| url | https://www.mdpi.com/2079-4991/12/17/2966 |
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