Association of nicotine dependence and gut microbiota: a bidirectional two-sample Mendelian randomization study
BackgroundNicotine dependence is a key factor influencing the diversity of gut microbiota, and targeting gut microbiota may become a new approach for the prevention and treatment of nicotine dependence. However, the causal relationship between the two is still unclear. This study aims to investigate...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1244272/full |
| _version_ | 1797635029910683648 |
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| author | Yuexuan Chen Mengjiao Zhao Kaisong Ji Jingjing Li Shuxin Wang Liming Lu Zhenhu Chen Jingchun Zeng |
| author_facet | Yuexuan Chen Mengjiao Zhao Kaisong Ji Jingjing Li Shuxin Wang Liming Lu Zhenhu Chen Jingchun Zeng |
| author_sort | Yuexuan Chen |
| collection | DOAJ |
| description | BackgroundNicotine dependence is a key factor influencing the diversity of gut microbiota, and targeting gut microbiota may become a new approach for the prevention and treatment of nicotine dependence. However, the causal relationship between the two is still unclear. This study aims to investigate the causal relationship between nicotine dependence and gut microbiota.MethodsA two-sample bidirectional Mendelian randomization (MR) study was conducted using the largest existing gut microbiota and nicotine dependence genome-wide association studies (GWAS). Causal relationships between genetically predicted nicotine dependence and gut microbiota abundance were examined using inverse variance weighted, MR-Egger, weighted median, simple mode, weighted mode, and MR-PRESSO approaches. Cochrane’s Q test, MR-Egger intercept test, and leave-one-out analysis were performed as sensitivity analyses to assess the robustness of the results. Multivariable Mendelian randomization analysis was also conducted to eliminate the interference of smoking-related phenotypes. Reverse Mendelian randomization analysis was then performed to determine the causal relationship between genetically predicted gut microbiota abundance and nicotine dependence.ResultsGenetically predicted nicotine dependence had a causal effect on Christensenellaceae (β: -0.52, 95% CI: -0.934–0.106, P = 0.014). The Eubacterium xylanophilum group (OR: 1.106, 95% CI: 1.004-1.218), Lachnoclostridium (OR: 1.118, 95% CI: 1.001-1.249) and Holdemania (OR: 1.08, 95% CI: 1.001-1.167) were risk factors for nicotine dependence. Peptostreptococcaceae (OR: 0.905, 95% CI: 0.837-0.977), Desulfovibrio (OR: 0.014, 95% CI: 0.819-0.977), Dorea (OR: 0.841, 95% CI. 0.731-0.968), Faecalibacterium (OR: 0.831, 95% CI: 0.735-0.939) and Sutterella (OR: 0.838, 95% CI: 0.739-0.951) were protective factor for nicotine dependence. The sensitivity analysis showed consistent results.ConclusionThe Mendelian randomization study confirmed the causal link between genetically predicted risk of nicotine dependence and genetically predicted abundance of gut microbiota. Gut microbiota may serve as a biomarker and offer insights for addressing nicotine dependence. |
| first_indexed | 2024-03-11T12:16:29Z |
| format | Article |
| id | doaj.art-2d5208b8200745358081bd797606887f |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-11T12:16:29Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-2d5208b8200745358081bd797606887f2023-11-07T07:59:35ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-11-011410.3389/fimmu.2023.12442721244272Association of nicotine dependence and gut microbiota: a bidirectional two-sample Mendelian randomization studyYuexuan Chen0Mengjiao Zhao1Kaisong Ji2Jingjing Li3Shuxin Wang4Liming Lu5Zhenhu Chen6Jingchun Zeng7The First Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSouth China Research Center for Acupuncture and Moxibustion, Medical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Acupuncture, Baoan District Hospital of Traditional Chinese Medicine, Shenzhen, ChinaDepartment of Acupuncture, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaSouth China Research Center for Acupuncture and Moxibustion, Medical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Acupuncture, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Acupuncture, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaBackgroundNicotine dependence is a key factor influencing the diversity of gut microbiota, and targeting gut microbiota may become a new approach for the prevention and treatment of nicotine dependence. However, the causal relationship between the two is still unclear. This study aims to investigate the causal relationship between nicotine dependence and gut microbiota.MethodsA two-sample bidirectional Mendelian randomization (MR) study was conducted using the largest existing gut microbiota and nicotine dependence genome-wide association studies (GWAS). Causal relationships between genetically predicted nicotine dependence and gut microbiota abundance were examined using inverse variance weighted, MR-Egger, weighted median, simple mode, weighted mode, and MR-PRESSO approaches. Cochrane’s Q test, MR-Egger intercept test, and leave-one-out analysis were performed as sensitivity analyses to assess the robustness of the results. Multivariable Mendelian randomization analysis was also conducted to eliminate the interference of smoking-related phenotypes. Reverse Mendelian randomization analysis was then performed to determine the causal relationship between genetically predicted gut microbiota abundance and nicotine dependence.ResultsGenetically predicted nicotine dependence had a causal effect on Christensenellaceae (β: -0.52, 95% CI: -0.934–0.106, P = 0.014). The Eubacterium xylanophilum group (OR: 1.106, 95% CI: 1.004-1.218), Lachnoclostridium (OR: 1.118, 95% CI: 1.001-1.249) and Holdemania (OR: 1.08, 95% CI: 1.001-1.167) were risk factors for nicotine dependence. Peptostreptococcaceae (OR: 0.905, 95% CI: 0.837-0.977), Desulfovibrio (OR: 0.014, 95% CI: 0.819-0.977), Dorea (OR: 0.841, 95% CI. 0.731-0.968), Faecalibacterium (OR: 0.831, 95% CI: 0.735-0.939) and Sutterella (OR: 0.838, 95% CI: 0.739-0.951) were protective factor for nicotine dependence. The sensitivity analysis showed consistent results.ConclusionThe Mendelian randomization study confirmed the causal link between genetically predicted risk of nicotine dependence and genetically predicted abundance of gut microbiota. Gut microbiota may serve as a biomarker and offer insights for addressing nicotine dependence.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1244272/fullgut microbiotaMendelian randomizationnicotine dependencecausalitygenetics |
| spellingShingle | Yuexuan Chen Mengjiao Zhao Kaisong Ji Jingjing Li Shuxin Wang Liming Lu Zhenhu Chen Jingchun Zeng Association of nicotine dependence and gut microbiota: a bidirectional two-sample Mendelian randomization study Frontiers in Immunology gut microbiota Mendelian randomization nicotine dependence causality genetics |
| title | Association of nicotine dependence and gut microbiota: a bidirectional two-sample Mendelian randomization study |
| title_full | Association of nicotine dependence and gut microbiota: a bidirectional two-sample Mendelian randomization study |
| title_fullStr | Association of nicotine dependence and gut microbiota: a bidirectional two-sample Mendelian randomization study |
| title_full_unstemmed | Association of nicotine dependence and gut microbiota: a bidirectional two-sample Mendelian randomization study |
| title_short | Association of nicotine dependence and gut microbiota: a bidirectional two-sample Mendelian randomization study |
| title_sort | association of nicotine dependence and gut microbiota a bidirectional two sample mendelian randomization study |
| topic | gut microbiota Mendelian randomization nicotine dependence causality genetics |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1244272/full |
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