A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane.

A sensitive and noble amperometric horseradish peroxidase (HRP) biosensor is fabricated via the deposition of gold nanoparticles (AuNPs) onto a three-dimensional (3D) porous carbonized chicken eggshell membrane (CESM). Due to the synergistic effects of the unique porous carbon architecture and well-...

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Main Authors: Di Zhang, He Zhao, Zhuangjun Fan, Mingjie Li, Penghui Du, Chenming Liu, Yuping Li, Haitao Li, Hongbin Cao
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0130156
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author Di Zhang
He Zhao
Zhuangjun Fan
Mingjie Li
Penghui Du
Chenming Liu
Yuping Li
Haitao Li
Hongbin Cao
author_facet Di Zhang
He Zhao
Zhuangjun Fan
Mingjie Li
Penghui Du
Chenming Liu
Yuping Li
Haitao Li
Hongbin Cao
author_sort Di Zhang
collection DOAJ
description A sensitive and noble amperometric horseradish peroxidase (HRP) biosensor is fabricated via the deposition of gold nanoparticles (AuNPs) onto a three-dimensional (3D) porous carbonized chicken eggshell membrane (CESM). Due to the synergistic effects of the unique porous carbon architecture and well-distributed AuNPs, the enzyme-modified electrode shows an excellent electrochemical redox behavior. Compared with bare glass carbon electrode (GCE), the cathodic peak current of the enzymatic electrode increases 12.6 times at a formal potential of -100 mV (vs. SCE) and charge-transfer resistance decreases 62.8%. Additionally, the AuNPs-CESM electrode exhibits a good biocompatibility, which effectively retains its bioactivity with a surface coverage of HRP 6.39×10(-9) mol cm(-2) (752 times higher than the theoretical monolayer coverage of HRP). Furthermore, the HRP-AuNPs-CESM-GCE electrode, as a biosensor for H2O2 detection, has a good accuracy and high sensitivity with the linear range of 0.01-2.7 mM H2O2 and the detection limit of 3 μM H2O2 (S/N = 3).
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spelling doaj.art-2d541d73fa1448b4a5a2553a5040e61b2022-12-21T18:33:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e013015610.1371/journal.pone.0130156A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane.Di ZhangHe ZhaoZhuangjun FanMingjie LiPenghui DuChenming LiuYuping LiHaitao LiHongbin CaoA sensitive and noble amperometric horseradish peroxidase (HRP) biosensor is fabricated via the deposition of gold nanoparticles (AuNPs) onto a three-dimensional (3D) porous carbonized chicken eggshell membrane (CESM). Due to the synergistic effects of the unique porous carbon architecture and well-distributed AuNPs, the enzyme-modified electrode shows an excellent electrochemical redox behavior. Compared with bare glass carbon electrode (GCE), the cathodic peak current of the enzymatic electrode increases 12.6 times at a formal potential of -100 mV (vs. SCE) and charge-transfer resistance decreases 62.8%. Additionally, the AuNPs-CESM electrode exhibits a good biocompatibility, which effectively retains its bioactivity with a surface coverage of HRP 6.39×10(-9) mol cm(-2) (752 times higher than the theoretical monolayer coverage of HRP). Furthermore, the HRP-AuNPs-CESM-GCE electrode, as a biosensor for H2O2 detection, has a good accuracy and high sensitivity with the linear range of 0.01-2.7 mM H2O2 and the detection limit of 3 μM H2O2 (S/N = 3).https://doi.org/10.1371/journal.pone.0130156
spellingShingle Di Zhang
He Zhao
Zhuangjun Fan
Mingjie Li
Penghui Du
Chenming Liu
Yuping Li
Haitao Li
Hongbin Cao
A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane.
PLoS ONE
title A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane.
title_full A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane.
title_fullStr A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane.
title_full_unstemmed A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane.
title_short A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane.
title_sort highly sensitive and selective hydrogen peroxide biosensor based on gold nanoparticles and three dimensional porous carbonized chicken eggshell membrane
url https://doi.org/10.1371/journal.pone.0130156
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