Combination of FACS and homologous recombination for the generation of stable and high-expression engineered cell lines.
Traditionally, cell line generation requires several months and involves screening of over several hundred cell clones for high productivity before dozens are selected as candidate cell lines. Here, we have designed a new strategy for the generation of stable and high-expression cell lines by combin...
Main Authors: | Lei Shi, Xuesi Chen, Wenying Tang, Zhenyi Li, Jin Liu, Feng Gao, Jianli Sang |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3960159?pdf=render |
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