Histological and electrophysiological evidence on the safe operation of a sharp-tip multimodal optrode during infrared neuromodulation of the rat cortex
Abstract Infrared neuromodulation is an emerging technology in neuroscience that exploits the inherent thermal sensitivity of neurons to excite or inhibit cellular activity. Since there is limited information on the physiological response of intracortical cell population in vivo including evidence o...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-15367-4 |
| _version_ | 1811224225782956032 |
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| author | Á. Cs. Horváth S. Borbély F. Mihók P. Fürjes P. Barthó Z. Fekete |
| author_facet | Á. Cs. Horváth S. Borbély F. Mihók P. Fürjes P. Barthó Z. Fekete |
| author_sort | Á. Cs. Horváth |
| collection | DOAJ |
| description | Abstract Infrared neuromodulation is an emerging technology in neuroscience that exploits the inherent thermal sensitivity of neurons to excite or inhibit cellular activity. Since there is limited information on the physiological response of intracortical cell population in vivo including evidence on cell damage, we aimed to create and to validate the safe operation of a microscale sharp-tip implantable optrode that can be used to suppress the activity of neuronal population with low optical power continuous wave irradiation. Effective thermal cross-section and electric properties of the multimodal microdevice was characterized in bench-top tests. The evoked multi-unit activity was monitored in the rat somatosensory cortex, and using NeuN immunocytochemistry method, quantitative analysis of neuronal density changes due to the stimulation trials was evaluated. The sharp tip implant was effectively used to suppress the firing rate of neuronal populations. Histological staining showed that neither the probe insertion nor the heating protocols alone lead to significant changes in cell density in the close vicinity of the implant with respect to the intact control region. Our study shows that intracortical stimulation with continuous-wave infrared light at 1550 nm using a sharp tip implantable optical microdevice is a safe approach to modulate the firing rate of neurons. |
| first_indexed | 2024-04-12T08:44:49Z |
| format | Article |
| id | doaj.art-2d589707b8dc457e8810b0036c92813b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T08:44:49Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2d589707b8dc457e8810b0036c92813b2022-12-22T03:39:44ZengNature PortfolioScientific Reports2045-23222022-07-0112111010.1038/s41598-022-15367-4Histological and electrophysiological evidence on the safe operation of a sharp-tip multimodal optrode during infrared neuromodulation of the rat cortexÁ. Cs. Horváth0S. Borbély1F. Mihók2P. Fürjes3P. Barthó4Z. Fekete5Research Group for Implantable Microsystems, Faculty of Information Technology and Bionics, PPKESleep Oscillations Research Group, Institute of Cognitive Neuroscience and Psychology, RCNS, ELKHDepartment of Control Engineering and Information Technology, BUTEMicrosystems Laboratory, Centre for Energy Research, ELKHSleep Oscillations Research Group, Institute of Cognitive Neuroscience and Psychology, RCNS, ELKHResearch Group for Implantable Microsystems, Faculty of Information Technology and Bionics, PPKEAbstract Infrared neuromodulation is an emerging technology in neuroscience that exploits the inherent thermal sensitivity of neurons to excite or inhibit cellular activity. Since there is limited information on the physiological response of intracortical cell population in vivo including evidence on cell damage, we aimed to create and to validate the safe operation of a microscale sharp-tip implantable optrode that can be used to suppress the activity of neuronal population with low optical power continuous wave irradiation. Effective thermal cross-section and electric properties of the multimodal microdevice was characterized in bench-top tests. The evoked multi-unit activity was monitored in the rat somatosensory cortex, and using NeuN immunocytochemistry method, quantitative analysis of neuronal density changes due to the stimulation trials was evaluated. The sharp tip implant was effectively used to suppress the firing rate of neuronal populations. Histological staining showed that neither the probe insertion nor the heating protocols alone lead to significant changes in cell density in the close vicinity of the implant with respect to the intact control region. Our study shows that intracortical stimulation with continuous-wave infrared light at 1550 nm using a sharp tip implantable optical microdevice is a safe approach to modulate the firing rate of neurons.https://doi.org/10.1038/s41598-022-15367-4 |
| spellingShingle | Á. Cs. Horváth S. Borbély F. Mihók P. Fürjes P. Barthó Z. Fekete Histological and electrophysiological evidence on the safe operation of a sharp-tip multimodal optrode during infrared neuromodulation of the rat cortex Scientific Reports |
| title | Histological and electrophysiological evidence on the safe operation of a sharp-tip multimodal optrode during infrared neuromodulation of the rat cortex |
| title_full | Histological and electrophysiological evidence on the safe operation of a sharp-tip multimodal optrode during infrared neuromodulation of the rat cortex |
| title_fullStr | Histological and electrophysiological evidence on the safe operation of a sharp-tip multimodal optrode during infrared neuromodulation of the rat cortex |
| title_full_unstemmed | Histological and electrophysiological evidence on the safe operation of a sharp-tip multimodal optrode during infrared neuromodulation of the rat cortex |
| title_short | Histological and electrophysiological evidence on the safe operation of a sharp-tip multimodal optrode during infrared neuromodulation of the rat cortex |
| title_sort | histological and electrophysiological evidence on the safe operation of a sharp tip multimodal optrode during infrared neuromodulation of the rat cortex |
| url | https://doi.org/10.1038/s41598-022-15367-4 |
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