Hypermucoviscous Carbapenem-Resistant <i>Klebsiella pneumoniae</i> ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation
<i>Klebsiella pneumoniae</i> is an opportunistic pathogen that can produce moderate and severe infections in immunosuppressed hosts. In recent years, an increase in the isolation of hypermucoviscous carbapenem-resistant <i>K. pneumoniae</i> with sequence type 25 (ST25) in hos...
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| Language: | English |
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/8804 |
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| author | Stefania Dentice Maidana Mariano Elean Kohtaro Fukuyama Yoshiya Imamura Leonardo Albarracín Sudeb Saha Yoshihito Suda Shoichiro Kurata María Ángela Jure Haruki Kitazawa Julio Villena |
| author_facet | Stefania Dentice Maidana Mariano Elean Kohtaro Fukuyama Yoshiya Imamura Leonardo Albarracín Sudeb Saha Yoshihito Suda Shoichiro Kurata María Ángela Jure Haruki Kitazawa Julio Villena |
| author_sort | Stefania Dentice Maidana |
| collection | DOAJ |
| description | <i>Klebsiella pneumoniae</i> is an opportunistic pathogen that can produce moderate and severe infections in immunosuppressed hosts. In recent years, an increase in the isolation of hypermucoviscous carbapenem-resistant <i>K. pneumoniae</i> with sequence type 25 (ST25) in hospitals in Norwest Argentina was observed. This work aimed to study the virulence and inflammatory potential of two <i>K. pneumoniae</i> ST25 strains (LABACER01 and LABACER27) in the intestinal mucosa. The human intestinal Caco-2 cells were infected with the <i>K. pneumoniae</i> ST25 strains, and their adhesion and invasion rates and changes in the expression of tight junction and inflammatory factors genes were evaluated. ST25 strains were able to adhere and invade Caco-2 cells, reducing their viability. Furthermore, both strains reduced the expression of tight junction proteins (occludin, ZO-1, and claudin-5), altered permeability, and increased the expression of TGF-β and TLL1 and the inflammatory factors (COX-2, iNOS, MCP-1, IL-6, IL-8, and TNF-α) in Caco-2 cells. The inflammatory response induced by LABACER01 and LABACER27 was significantly lower than the one produced by LPS or other intestinal pathogens, including <i>K. pneumoniae</i> NTUH-K2044. No differences in virulence and inflammatory potential were found between LABACER01 and LABACER27. In line with these findings, no major differences between the strains were found when the comparative genomic analysis of virulence factors associated with intestinal infection/colonization was performed. This work is the first to demonstrate that hypermucoviscous carbapenem-resistant <i>K. pneumoniae</i> ST25 infects human intestinal epithelial cells and induces moderate inflammation. |
| first_indexed | 2024-03-11T03:39:20Z |
| format | Article |
| id | doaj.art-2d5a708ddff44fe1a9384ba05ff608e1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:39:20Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2d5a708ddff44fe1a9384ba05ff608e12023-11-18T01:42:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410880410.3390/ijms24108804Hypermucoviscous Carbapenem-Resistant <i>Klebsiella pneumoniae</i> ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate InflammationStefania Dentice Maidana0Mariano Elean1Kohtaro Fukuyama2Yoshiya Imamura3Leonardo Albarracín4Sudeb Saha5Yoshihito Suda6Shoichiro Kurata7María Ángela Jure8Haruki Kitazawa9Julio Villena10Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, ArgentinaLaboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, ArgentinaFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, JapanFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, JapanLaboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, ArgentinaFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, JapanDepartment of Food, Agriculture and Environment, Miyagi University, Sendai 980-8572, JapanLaboratory of Molecular Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, JapanLaboratory of Antimicrobials, Institute of Microbiology “Luis C. Verna”, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucuman, Tucuman 4000, ArgentinaFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, JapanLaboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina<i>Klebsiella pneumoniae</i> is an opportunistic pathogen that can produce moderate and severe infections in immunosuppressed hosts. In recent years, an increase in the isolation of hypermucoviscous carbapenem-resistant <i>K. pneumoniae</i> with sequence type 25 (ST25) in hospitals in Norwest Argentina was observed. This work aimed to study the virulence and inflammatory potential of two <i>K. pneumoniae</i> ST25 strains (LABACER01 and LABACER27) in the intestinal mucosa. The human intestinal Caco-2 cells were infected with the <i>K. pneumoniae</i> ST25 strains, and their adhesion and invasion rates and changes in the expression of tight junction and inflammatory factors genes were evaluated. ST25 strains were able to adhere and invade Caco-2 cells, reducing their viability. Furthermore, both strains reduced the expression of tight junction proteins (occludin, ZO-1, and claudin-5), altered permeability, and increased the expression of TGF-β and TLL1 and the inflammatory factors (COX-2, iNOS, MCP-1, IL-6, IL-8, and TNF-α) in Caco-2 cells. The inflammatory response induced by LABACER01 and LABACER27 was significantly lower than the one produced by LPS or other intestinal pathogens, including <i>K. pneumoniae</i> NTUH-K2044. No differences in virulence and inflammatory potential were found between LABACER01 and LABACER27. In line with these findings, no major differences between the strains were found when the comparative genomic analysis of virulence factors associated with intestinal infection/colonization was performed. This work is the first to demonstrate that hypermucoviscous carbapenem-resistant <i>K. pneumoniae</i> ST25 infects human intestinal epithelial cells and induces moderate inflammation.https://www.mdpi.com/1422-0067/24/10/8804<i>Klebsiella pneumoniae</i>carbapenem resistantintestinal infectiongenomicsequence type 25 |
| spellingShingle | Stefania Dentice Maidana Mariano Elean Kohtaro Fukuyama Yoshiya Imamura Leonardo Albarracín Sudeb Saha Yoshihito Suda Shoichiro Kurata María Ángela Jure Haruki Kitazawa Julio Villena Hypermucoviscous Carbapenem-Resistant <i>Klebsiella pneumoniae</i> ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation International Journal of Molecular Sciences <i>Klebsiella pneumoniae</i> carbapenem resistant intestinal infection genomic sequence type 25 |
| title | Hypermucoviscous Carbapenem-Resistant <i>Klebsiella pneumoniae</i> ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation |
| title_full | Hypermucoviscous Carbapenem-Resistant <i>Klebsiella pneumoniae</i> ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation |
| title_fullStr | Hypermucoviscous Carbapenem-Resistant <i>Klebsiella pneumoniae</i> ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation |
| title_full_unstemmed | Hypermucoviscous Carbapenem-Resistant <i>Klebsiella pneumoniae</i> ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation |
| title_short | Hypermucoviscous Carbapenem-Resistant <i>Klebsiella pneumoniae</i> ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation |
| title_sort | hypermucoviscous carbapenem resistant i klebsiella pneumoniae i st25 infect human intestinal epithelial cells and induce moderate inflammation |
| topic | <i>Klebsiella pneumoniae</i> carbapenem resistant intestinal infection genomic sequence type 25 |
| url | https://www.mdpi.com/1422-0067/24/10/8804 |
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