Rapid and label-free, electrochemical DNA detection utilizing the oxidase-mimicking activity of cerium oxide nanoparticles
We herein developed a simple and ultrafast electrochemical DNA detection method by utilizing the oxidase-mimicking activity of cerium oxide nanoparticles (CeO2). In the sensor, the presence of DNA, which electrostatically interacts with CeO2 and 3,3′,5,5′-tetramethylbenzidine (TMB) substrate, limits...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-02-01
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| Series: | Electrochemistry Communications |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1388248118303230 |
| _version_ | 1818023511274291200 |
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| author | Hyo Yong Kim Jun Ki Ahn Moon Il Kim Ki Soo Park Hyun Gyu Park |
| author_facet | Hyo Yong Kim Jun Ki Ahn Moon Il Kim Ki Soo Park Hyun Gyu Park |
| author_sort | Hyo Yong Kim |
| collection | DOAJ |
| description | We herein developed a simple and ultrafast electrochemical DNA detection method by utilizing the oxidase-mimicking activity of cerium oxide nanoparticles (CeO2). In the sensor, the presence of DNA, which electrostatically interacts with CeO2 and 3,3′,5,5′-tetramethylbenzidine (TMB) substrate, limits the formation of CeO2-TMB complex that is required for the oxidase-like reaction. As a result, the oxidase-mimicking activity of CeO2 is not effectively exerted, leading to the significantly diminished electrochemical current signal. With this novel strategy, we successfully determined DNA amplicon derived from Escherichia coli (E. coli) with the total assay time less than 6 min even without the post-purification of DNA amplicon. Keywords: Cerium oxide nanoparticle, Oxidase-mimicking activity, Biosensor, DNA, Electrochemical detection |
| first_indexed | 2024-12-10T03:45:29Z |
| format | Article |
| id | doaj.art-2d5ceea119694f268473fcd2d33e43d3 |
| institution | Directory Open Access Journal |
| issn | 1388-2481 |
| language | English |
| last_indexed | 2024-12-10T03:45:29Z |
| publishDate | 2019-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electrochemistry Communications |
| spelling | doaj.art-2d5ceea119694f268473fcd2d33e43d32022-12-22T02:03:26ZengElsevierElectrochemistry Communications1388-24812019-02-0199510Rapid and label-free, electrochemical DNA detection utilizing the oxidase-mimicking activity of cerium oxide nanoparticlesHyo Yong Kim0Jun Ki Ahn1Moon Il Kim2Ki Soo Park3Hyun Gyu Park4Department of Chemical and Biomolecular Engineering (BK21+ Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of KoreaDepartment of Chemical and Biomolecular Engineering (BK21+ Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of KoreaDepartment of BioNano Technology, Gachon University, Gyeonggi 461-701, Republic of KoreaDepartment of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea; Corresponding authors.Department of Chemical and Biomolecular Engineering (BK21+ Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea; Corresponding authors.We herein developed a simple and ultrafast electrochemical DNA detection method by utilizing the oxidase-mimicking activity of cerium oxide nanoparticles (CeO2). In the sensor, the presence of DNA, which electrostatically interacts with CeO2 and 3,3′,5,5′-tetramethylbenzidine (TMB) substrate, limits the formation of CeO2-TMB complex that is required for the oxidase-like reaction. As a result, the oxidase-mimicking activity of CeO2 is not effectively exerted, leading to the significantly diminished electrochemical current signal. With this novel strategy, we successfully determined DNA amplicon derived from Escherichia coli (E. coli) with the total assay time less than 6 min even without the post-purification of DNA amplicon. Keywords: Cerium oxide nanoparticle, Oxidase-mimicking activity, Biosensor, DNA, Electrochemical detectionhttp://www.sciencedirect.com/science/article/pii/S1388248118303230 |
| spellingShingle | Hyo Yong Kim Jun Ki Ahn Moon Il Kim Ki Soo Park Hyun Gyu Park Rapid and label-free, electrochemical DNA detection utilizing the oxidase-mimicking activity of cerium oxide nanoparticles Electrochemistry Communications |
| title | Rapid and label-free, electrochemical DNA detection utilizing the oxidase-mimicking activity of cerium oxide nanoparticles |
| title_full | Rapid and label-free, electrochemical DNA detection utilizing the oxidase-mimicking activity of cerium oxide nanoparticles |
| title_fullStr | Rapid and label-free, electrochemical DNA detection utilizing the oxidase-mimicking activity of cerium oxide nanoparticles |
| title_full_unstemmed | Rapid and label-free, electrochemical DNA detection utilizing the oxidase-mimicking activity of cerium oxide nanoparticles |
| title_short | Rapid and label-free, electrochemical DNA detection utilizing the oxidase-mimicking activity of cerium oxide nanoparticles |
| title_sort | rapid and label free electrochemical dna detection utilizing the oxidase mimicking activity of cerium oxide nanoparticles |
| url | http://www.sciencedirect.com/science/article/pii/S1388248118303230 |
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