The dar Genes of Pseudomonas chlororaphis PCL1606 Are Crucial for Biocontrol Activity via Production of the Antifungal Compound 2-Hexyl, 5-Propyl Resorcinol
To determine the genetic basis by which 2-hexyl, 5-propyl resorcinol (HPR) is produced by the biocontrol rhizobacterium Pseudomonas chlororaphis (formerly known as P. fluorescens) PCL1606, the presence and role of dar genes were investigated. To accomplish this aim, the pCGNOV-1 plasmid was isolated...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2013-05-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-13-0012-R |
| _version_ | 1818646924017795072 |
|---|---|
| author | Claudia E. Calderón Alejandro Pérez-García Antonio de Vicente Francisco M. Cazorla |
| author_facet | Claudia E. Calderón Alejandro Pérez-García Antonio de Vicente Francisco M. Cazorla |
| author_sort | Claudia E. Calderón |
| collection | DOAJ |
| description | To determine the genetic basis by which 2-hexyl, 5-propyl resorcinol (HPR) is produced by the biocontrol rhizobacterium Pseudomonas chlororaphis (formerly known as P. fluorescens) PCL1606, the presence and role of dar genes were investigated. To accomplish this aim, the pCGNOV-1 plasmid was isolated from a PCL1606 genomic library and was shown to hybridize to various dar probes by Southern blot. An analysis of the pCGNOV-1 genomic DNA revealed the presence of five open reading frames that were homologous to dar genes and had an organization that resembled the arrangement of previously described P. chlororaphis strains. Phylogenetic studies resulted in the clustering of PCL1606 with the P. chlororaphis subgroup, which supported the renaming of this strain from P. fluorescens to P. chlororaphis PCL1606. The construction of insertional mutants for each homologous dar gene in P. chlororaphis PCL1606 along with their corresponding complemented derivative strains restored HPR production and confirmed the key role of the dar A and darB genes in HPR production and in the antagonistic phenotype. Finally, biocontrol assays were performed on avocado–Rosellinia and tomato–Fusarium test systems using the HPR-defective and -complemented derivative strains generated here and demonstrated the crucial role of the biosynthetic dar genes in the biocontrol phenotype of P. chlororaphis PCL1606. This biocontrol phenotype is dependent on the dar genes via their production of the HPR antibiotic. Some of the dar genes not directly involved in the biosynthesis of HPR, such as darS or darR, might contribute to regulatory features of HPR production. |
| first_indexed | 2024-12-17T00:54:22Z |
| format | Article |
| id | doaj.art-2d5f93f62c5d42d6834578f58df55faf |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T00:54:22Z |
| publishDate | 2013-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-2d5f93f62c5d42d6834578f58df55faf2022-12-21T22:09:38ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062013-05-0126555456510.1094/MPMI-01-13-0012-RThe dar Genes of Pseudomonas chlororaphis PCL1606 Are Crucial for Biocontrol Activity via Production of the Antifungal Compound 2-Hexyl, 5-Propyl ResorcinolClaudia E. CalderónAlejandro Pérez-GarcíaAntonio de VicenteFrancisco M. CazorlaTo determine the genetic basis by which 2-hexyl, 5-propyl resorcinol (HPR) is produced by the biocontrol rhizobacterium Pseudomonas chlororaphis (formerly known as P. fluorescens) PCL1606, the presence and role of dar genes were investigated. To accomplish this aim, the pCGNOV-1 plasmid was isolated from a PCL1606 genomic library and was shown to hybridize to various dar probes by Southern blot. An analysis of the pCGNOV-1 genomic DNA revealed the presence of five open reading frames that were homologous to dar genes and had an organization that resembled the arrangement of previously described P. chlororaphis strains. Phylogenetic studies resulted in the clustering of PCL1606 with the P. chlororaphis subgroup, which supported the renaming of this strain from P. fluorescens to P. chlororaphis PCL1606. The construction of insertional mutants for each homologous dar gene in P. chlororaphis PCL1606 along with their corresponding complemented derivative strains restored HPR production and confirmed the key role of the dar A and darB genes in HPR production and in the antagonistic phenotype. Finally, biocontrol assays were performed on avocado–Rosellinia and tomato–Fusarium test systems using the HPR-defective and -complemented derivative strains generated here and demonstrated the crucial role of the biosynthetic dar genes in the biocontrol phenotype of P. chlororaphis PCL1606. This biocontrol phenotype is dependent on the dar genes via their production of the HPR antibiotic. Some of the dar genes not directly involved in the biosynthesis of HPR, such as darS or darR, might contribute to regulatory features of HPR production.https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-13-0012-R |
| spellingShingle | Claudia E. Calderón Alejandro Pérez-García Antonio de Vicente Francisco M. Cazorla The dar Genes of Pseudomonas chlororaphis PCL1606 Are Crucial for Biocontrol Activity via Production of the Antifungal Compound 2-Hexyl, 5-Propyl Resorcinol Molecular Plant-Microbe Interactions |
| title | The dar Genes of Pseudomonas chlororaphis PCL1606 Are Crucial for Biocontrol Activity via Production of the Antifungal Compound 2-Hexyl, 5-Propyl Resorcinol |
| title_full | The dar Genes of Pseudomonas chlororaphis PCL1606 Are Crucial for Biocontrol Activity via Production of the Antifungal Compound 2-Hexyl, 5-Propyl Resorcinol |
| title_fullStr | The dar Genes of Pseudomonas chlororaphis PCL1606 Are Crucial for Biocontrol Activity via Production of the Antifungal Compound 2-Hexyl, 5-Propyl Resorcinol |
| title_full_unstemmed | The dar Genes of Pseudomonas chlororaphis PCL1606 Are Crucial for Biocontrol Activity via Production of the Antifungal Compound 2-Hexyl, 5-Propyl Resorcinol |
| title_short | The dar Genes of Pseudomonas chlororaphis PCL1606 Are Crucial for Biocontrol Activity via Production of the Antifungal Compound 2-Hexyl, 5-Propyl Resorcinol |
| title_sort | dar genes of pseudomonas chlororaphis pcl1606 are crucial for biocontrol activity via production of the antifungal compound 2 hexyl 5 propyl resorcinol |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-13-0012-R |
| work_keys_str_mv | AT claudiaecalderon thedargenesofpseudomonaschlororaphispcl1606arecrucialforbiocontrolactivityviaproductionoftheantifungalcompound2hexyl5propylresorcinol AT alejandroperezgarcia thedargenesofpseudomonaschlororaphispcl1606arecrucialforbiocontrolactivityviaproductionoftheantifungalcompound2hexyl5propylresorcinol AT antoniodevicente thedargenesofpseudomonaschlororaphispcl1606arecrucialforbiocontrolactivityviaproductionoftheantifungalcompound2hexyl5propylresorcinol AT franciscomcazorla thedargenesofpseudomonaschlororaphispcl1606arecrucialforbiocontrolactivityviaproductionoftheantifungalcompound2hexyl5propylresorcinol AT claudiaecalderon dargenesofpseudomonaschlororaphispcl1606arecrucialforbiocontrolactivityviaproductionoftheantifungalcompound2hexyl5propylresorcinol AT alejandroperezgarcia dargenesofpseudomonaschlororaphispcl1606arecrucialforbiocontrolactivityviaproductionoftheantifungalcompound2hexyl5propylresorcinol AT antoniodevicente dargenesofpseudomonaschlororaphispcl1606arecrucialforbiocontrolactivityviaproductionoftheantifungalcompound2hexyl5propylresorcinol AT franciscomcazorla dargenesofpseudomonaschlororaphispcl1606arecrucialforbiocontrolactivityviaproductionoftheantifungalcompound2hexyl5propylresorcinol |