Monitoring Ion Activities In and Around Cells Using Ion-Selective Liquid-Membrane Microelectrodes
Determining the effective concentration (i.e., activity) of ions in and around living cells is important to our understanding of the contribution of those ions to cellular function. Moreover, monitoring changes in ion activities in and around cells is informative about the actions of the transporter...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-01-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/13/1/984 |
| _version_ | 1798043681312210944 |
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| author | Mark D. Parker Walter F. Boron Seong-Ki Lee |
| author_facet | Mark D. Parker Walter F. Boron Seong-Ki Lee |
| author_sort | Mark D. Parker |
| collection | DOAJ |
| description | Determining the effective concentration (i.e., activity) of ions in and around living cells is important to our understanding of the contribution of those ions to cellular function. Moreover, monitoring changes in ion activities in and around cells is informative about the actions of the transporters and/or channels operating in the cell membrane. The activity of an ion can be measured using a glass microelectrode that includes in its tip a liquid-membrane doped with an ion-selective ionophore. Because these electrodes can be fabricated with tip diameters that are less than 1 μm, they can be used to impale single cells in order to monitor the activities of intracellular ions. This review summarizes the history, theory, and practice of ion-selective microelectrode use and brings together a number of classic and recent examples of their usefulness in the realm of physiological study. |
| first_indexed | 2024-04-11T22:52:31Z |
| format | Article |
| id | doaj.art-2d6259ae3cef4220b4495a27367ddbe5 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:52:31Z |
| publishDate | 2013-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-2d6259ae3cef4220b4495a27367ddbe52022-12-22T03:58:32ZengMDPI AGSensors1424-82202013-01-01131984100310.3390/s130100984Monitoring Ion Activities In and Around Cells Using Ion-Selective Liquid-Membrane MicroelectrodesMark D. ParkerWalter F. BoronSeong-Ki LeeDetermining the effective concentration (i.e., activity) of ions in and around living cells is important to our understanding of the contribution of those ions to cellular function. Moreover, monitoring changes in ion activities in and around cells is informative about the actions of the transporters and/or channels operating in the cell membrane. The activity of an ion can be measured using a glass microelectrode that includes in its tip a liquid-membrane doped with an ion-selective ionophore. Because these electrodes can be fabricated with tip diameters that are less than 1 μm, they can be used to impale single cells in order to monitor the activities of intracellular ions. This review summarizes the history, theory, and practice of ion-selective microelectrode use and brings together a number of classic and recent examples of their usefulness in the realm of physiological study.http://www.mdpi.com/1424-8220/13/1/984ISMLIXNernst equation |
| spellingShingle | Mark D. Parker Walter F. Boron Seong-Ki Lee Monitoring Ion Activities In and Around Cells Using Ion-Selective Liquid-Membrane Microelectrodes Sensors ISM LIX Nernst equation |
| title | Monitoring Ion Activities In and Around Cells Using Ion-Selective Liquid-Membrane Microelectrodes |
| title_full | Monitoring Ion Activities In and Around Cells Using Ion-Selective Liquid-Membrane Microelectrodes |
| title_fullStr | Monitoring Ion Activities In and Around Cells Using Ion-Selective Liquid-Membrane Microelectrodes |
| title_full_unstemmed | Monitoring Ion Activities In and Around Cells Using Ion-Selective Liquid-Membrane Microelectrodes |
| title_short | Monitoring Ion Activities In and Around Cells Using Ion-Selective Liquid-Membrane Microelectrodes |
| title_sort | monitoring ion activities in and around cells using ion selective liquid membrane microelectrodes |
| topic | ISM LIX Nernst equation |
| url | http://www.mdpi.com/1424-8220/13/1/984 |
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