Nonirradiated NOD,B6.SCID Il2rγ−/− KitW41/W41 (NBSGW) Mice Support Multilineage Engraftment of Human Hematopoietic Cells
In this study, we demonstrate a newly derived mouse model that supports engraftment of human hematopoietic stem cells (HSCs) in the absence of irradiation. We cross the NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) strain with the C57BL/6J-KitW-41J/J (C57BL/6.KitW41) strain and engraft, without irradiation,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-02-01
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| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671114003828 |
| _version_ | 1819055962422509568 |
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| author | Brian E. McIntosh Matthew E. Brown Bret M. Duffin John P. Maufort David T. Vereide Igor I. Slukvin James A. Thomson |
| author_facet | Brian E. McIntosh Matthew E. Brown Bret M. Duffin John P. Maufort David T. Vereide Igor I. Slukvin James A. Thomson |
| author_sort | Brian E. McIntosh |
| collection | DOAJ |
| description | In this study, we demonstrate a newly derived mouse model that supports engraftment of human hematopoietic stem cells (HSCs) in the absence of irradiation. We cross the NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) strain with the C57BL/6J-KitW-41J/J (C57BL/6.KitW41) strain and engraft, without irradiation, the resulting NBSGW strain with human cord blood CD34+ cells. At 12-weeks postengraftment in NBSGW mice, we observe human cell chimerism in marrow (97% ± 0.4%), peripheral blood (61% ± 2%), and spleen (94% ± 2%) at levels observed with irradiation in NSG mice. We also detected a significant number of glycophorin-A-positive expressing cells in the developing NBSGW marrow. Further, the observed levels of human hematopoietic chimerism mimic those reported for both irradiated NSG and NSG-transgenic strains. This mouse model permits HSC engraftment while avoiding the complicating hematopoietic, gastrointestinal, and neurological side effects associated with irradiation and allows investigators without access to radiation to pursue engraftment studies with human HSCs. |
| first_indexed | 2024-12-21T13:15:51Z |
| format | Article |
| id | doaj.art-2d630842a39c43368e62ccb99ba8c2eb |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-21T13:15:51Z |
| publishDate | 2015-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-2d630842a39c43368e62ccb99ba8c2eb2022-12-21T19:02:44ZengElsevierStem Cell Reports2213-67112015-02-014217118010.1016/j.stemcr.2014.12.005Nonirradiated NOD,B6.SCID Il2rγ−/− KitW41/W41 (NBSGW) Mice Support Multilineage Engraftment of Human Hematopoietic CellsBrian E. McIntosh0Matthew E. Brown1Bret M. Duffin2John P. Maufort3David T. Vereide4Igor I. Slukvin5James A. Thomson6Morgridge Institute for Research, Madison, WI 53715, USADepartment of Surgery, University of Wisconsin, Madison, WI 53715, USAMorgridge Institute for Research, Madison, WI 53715, USAMorgridge Institute for Research, Madison, WI 53715, USAMorgridge Institute for Research, Madison, WI 53715, USAWisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USAMorgridge Institute for Research, Madison, WI 53715, USAIn this study, we demonstrate a newly derived mouse model that supports engraftment of human hematopoietic stem cells (HSCs) in the absence of irradiation. We cross the NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) strain with the C57BL/6J-KitW-41J/J (C57BL/6.KitW41) strain and engraft, without irradiation, the resulting NBSGW strain with human cord blood CD34+ cells. At 12-weeks postengraftment in NBSGW mice, we observe human cell chimerism in marrow (97% ± 0.4%), peripheral blood (61% ± 2%), and spleen (94% ± 2%) at levels observed with irradiation in NSG mice. We also detected a significant number of glycophorin-A-positive expressing cells in the developing NBSGW marrow. Further, the observed levels of human hematopoietic chimerism mimic those reported for both irradiated NSG and NSG-transgenic strains. This mouse model permits HSC engraftment while avoiding the complicating hematopoietic, gastrointestinal, and neurological side effects associated with irradiation and allows investigators without access to radiation to pursue engraftment studies with human HSCs.http://www.sciencedirect.com/science/article/pii/S2213671114003828 |
| spellingShingle | Brian E. McIntosh Matthew E. Brown Bret M. Duffin John P. Maufort David T. Vereide Igor I. Slukvin James A. Thomson Nonirradiated NOD,B6.SCID Il2rγ−/− KitW41/W41 (NBSGW) Mice Support Multilineage Engraftment of Human Hematopoietic Cells Stem Cell Reports |
| title | Nonirradiated NOD,B6.SCID Il2rγ−/− KitW41/W41 (NBSGW) Mice Support Multilineage Engraftment of Human Hematopoietic Cells |
| title_full | Nonirradiated NOD,B6.SCID Il2rγ−/− KitW41/W41 (NBSGW) Mice Support Multilineage Engraftment of Human Hematopoietic Cells |
| title_fullStr | Nonirradiated NOD,B6.SCID Il2rγ−/− KitW41/W41 (NBSGW) Mice Support Multilineage Engraftment of Human Hematopoietic Cells |
| title_full_unstemmed | Nonirradiated NOD,B6.SCID Il2rγ−/− KitW41/W41 (NBSGW) Mice Support Multilineage Engraftment of Human Hematopoietic Cells |
| title_short | Nonirradiated NOD,B6.SCID Il2rγ−/− KitW41/W41 (NBSGW) Mice Support Multilineage Engraftment of Human Hematopoietic Cells |
| title_sort | nonirradiated nod b6 scid il2rγ kitw41 w41 nbsgw mice support multilineage engraftment of human hematopoietic cells |
| url | http://www.sciencedirect.com/science/article/pii/S2213671114003828 |
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