Biomimetic Remineralized Three-Dimensional Collagen Bone Matrices with an Enhanced Osteostimulating Effect
Bone grafts with a high potential for osseointegration, capable of providing a complete and effective regeneration of bone tissue, remain an urgent and unresolved issue. The presented work proposes an approach to develop composite biomimetic bone material for reconstructive surgery by deposition (re...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Biomimetics |
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| Online Access: | https://www.mdpi.com/2313-7673/8/1/91 |
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| author | Irina S. Fadeeva Anastasia Yu. Teterina Vladislav V. Minaychev Anatoliy S. Senotov Igor V. Smirnov Roman S. Fadeev Polina V. Smirnova Vladislav O. Menukhov Yana V. Lomovskaya Vladimir S. Akatov Sergey M. Barinov Vladimir S. Komlev |
| author_facet | Irina S. Fadeeva Anastasia Yu. Teterina Vladislav V. Minaychev Anatoliy S. Senotov Igor V. Smirnov Roman S. Fadeev Polina V. Smirnova Vladislav O. Menukhov Yana V. Lomovskaya Vladimir S. Akatov Sergey M. Barinov Vladimir S. Komlev |
| author_sort | Irina S. Fadeeva |
| collection | DOAJ |
| description | Bone grafts with a high potential for osseointegration, capable of providing a complete and effective regeneration of bone tissue, remain an urgent and unresolved issue. The presented work proposes an approach to develop composite biomimetic bone material for reconstructive surgery by deposition (remineralization) on the surface of high-purity, demineralized bone collagen matrix calcium phosphate layers. Histological and elemental analysis have shown reproduction of the bone tissue matrix architectonics, and a high-purity degree of the obtained collagen scaffolds; the cell culture and confocal microscopy have demonstrated a high biocompatibility of the materials obtained. Adsorption spectroscopy, scanning electron microscopy, microcomputed tomography (microCT) and infrared spectroscopy, and X-ray diffraction have proven the efficiency of the deposition of calcium phosphates on the surface of bone collagen scaffolds. Cell culture and confocal microscopy methods have shown high biocompatibility of both demineralized and remineralized bone matrices. In the model of heterotopic implantation in rats, at the term of seven weeks, an intensive intratrabecular infiltration of calcium phosphate precipitates, and a pronounced synthetic activity of osteoblast remodeling and rebuilding implanted materials, were revealed in remineralized bone collagen matrices in contrast to demineralized ones. Thus, remineralization of highly purified demineralized bone matrices significantly enhanced their osteostimulating ability. The data obtained are of interest for the creation of new highly effective osteoplastic materials for bone tissue regeneration and augmentation. |
| first_indexed | 2024-03-11T06:52:19Z |
| format | Article |
| id | doaj.art-2d67105234f1498cb6d1611d0a568ffd |
| institution | Directory Open Access Journal |
| issn | 2313-7673 |
| language | English |
| last_indexed | 2024-03-11T06:52:19Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomimetics |
| spelling | doaj.art-2d67105234f1498cb6d1611d0a568ffd2023-11-17T09:50:10ZengMDPI AGBiomimetics2313-76732023-02-01819110.3390/biomimetics8010091Biomimetic Remineralized Three-Dimensional Collagen Bone Matrices with an Enhanced Osteostimulating EffectIrina S. Fadeeva0Anastasia Yu. Teterina1Vladislav V. Minaychev2Anatoliy S. Senotov3Igor V. Smirnov4Roman S. Fadeev5Polina V. Smirnova6Vladislav O. Menukhov7Yana V. Lomovskaya8Vladimir S. Akatov9Sergey M. Barinov10Vladimir S. Komlev11Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, RussiaBaikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Leninskiy Prospect 49, Moscow 117334, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, RussiaBaikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Leninskiy Prospect 49, Moscow 117334, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, RussiaBaikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Leninskiy Prospect 49, Moscow 117334, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, RussiaBaikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Leninskiy Prospect 49, Moscow 117334, RussiaBaikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Leninskiy Prospect 49, Moscow 117334, RussiaBone grafts with a high potential for osseointegration, capable of providing a complete and effective regeneration of bone tissue, remain an urgent and unresolved issue. The presented work proposes an approach to develop composite biomimetic bone material for reconstructive surgery by deposition (remineralization) on the surface of high-purity, demineralized bone collagen matrix calcium phosphate layers. Histological and elemental analysis have shown reproduction of the bone tissue matrix architectonics, and a high-purity degree of the obtained collagen scaffolds; the cell culture and confocal microscopy have demonstrated a high biocompatibility of the materials obtained. Adsorption spectroscopy, scanning electron microscopy, microcomputed tomography (microCT) and infrared spectroscopy, and X-ray diffraction have proven the efficiency of the deposition of calcium phosphates on the surface of bone collagen scaffolds. Cell culture and confocal microscopy methods have shown high biocompatibility of both demineralized and remineralized bone matrices. In the model of heterotopic implantation in rats, at the term of seven weeks, an intensive intratrabecular infiltration of calcium phosphate precipitates, and a pronounced synthetic activity of osteoblast remodeling and rebuilding implanted materials, were revealed in remineralized bone collagen matrices in contrast to demineralized ones. Thus, remineralization of highly purified demineralized bone matrices significantly enhanced their osteostimulating ability. The data obtained are of interest for the creation of new highly effective osteoplastic materials for bone tissue regeneration and augmentation.https://www.mdpi.com/2313-7673/8/1/91bioactive materialsbone graftsscaffoldscalcium phosphatescoatingsbone tissue engineering |
| spellingShingle | Irina S. Fadeeva Anastasia Yu. Teterina Vladislav V. Minaychev Anatoliy S. Senotov Igor V. Smirnov Roman S. Fadeev Polina V. Smirnova Vladislav O. Menukhov Yana V. Lomovskaya Vladimir S. Akatov Sergey M. Barinov Vladimir S. Komlev Biomimetic Remineralized Three-Dimensional Collagen Bone Matrices with an Enhanced Osteostimulating Effect Biomimetics bioactive materials bone grafts scaffolds calcium phosphates coatings bone tissue engineering |
| title | Biomimetic Remineralized Three-Dimensional Collagen Bone Matrices with an Enhanced Osteostimulating Effect |
| title_full | Biomimetic Remineralized Three-Dimensional Collagen Bone Matrices with an Enhanced Osteostimulating Effect |
| title_fullStr | Biomimetic Remineralized Three-Dimensional Collagen Bone Matrices with an Enhanced Osteostimulating Effect |
| title_full_unstemmed | Biomimetic Remineralized Three-Dimensional Collagen Bone Matrices with an Enhanced Osteostimulating Effect |
| title_short | Biomimetic Remineralized Three-Dimensional Collagen Bone Matrices with an Enhanced Osteostimulating Effect |
| title_sort | biomimetic remineralized three dimensional collagen bone matrices with an enhanced osteostimulating effect |
| topic | bioactive materials bone grafts scaffolds calcium phosphates coatings bone tissue engineering |
| url | https://www.mdpi.com/2313-7673/8/1/91 |
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