Effect of Chitosan-Diosgenin Combination on Wound Healing
The difficult-to-heal wounds continue to be a problem for modern medicine. Chitosan and diosgenin possess anti-inflammatory and antioxidant effects making them relevant substances for wound treatment. That is why this work aimed to study the effect of the combined application of chitosan and diosgen...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/5/5049 |
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| author | Lubomir Petrov Olya Stoilova Georgi Pramatarov Hristiyana Kanzova Elina Tsvetanova Madlena Andreeva Almira Georgieva Dimitrinka Atanasova Stanislav Philipov Albena Alexandrova |
| author_facet | Lubomir Petrov Olya Stoilova Georgi Pramatarov Hristiyana Kanzova Elina Tsvetanova Madlena Andreeva Almira Georgieva Dimitrinka Atanasova Stanislav Philipov Albena Alexandrova |
| author_sort | Lubomir Petrov |
| collection | DOAJ |
| description | The difficult-to-heal wounds continue to be a problem for modern medicine. Chitosan and diosgenin possess anti-inflammatory and antioxidant effects making them relevant substances for wound treatment. That is why this work aimed to study the effect of the combined application of chitosan and diosgenin on a mouse skin wound model. For the purpose, wounds (6 mm diameter) were made on mice’s backs and were treated for 9 days with one of the following: 50% ethanol (control), polyethylene glycol (PEG) in 50% ethanol, chitosan and PEG in 50% ethanol (Chs), diosgenin and PEG in 50% ethanol (Dg) and chitosan, diosgenin and PEG in 50% ethanol (ChsDg). Before the first treatment and on the 3rd, 6th and 9th days, the wounds were photographed and their area was determined. On the 9th day, animals were euthanized and wounds’ tissues were excised for histological analysis. In addition, the lipid peroxidation (LPO), protein oxidation (POx) and total glutathione (tGSH) levels were measured. The results showed that ChsDg had the most pronounced overall effect on wound area reduction, followed by Chs and PEG. Moreover, the application of ChsDg maintained high levels of tGSH in wound tissues, compared to other substances. It was shown that all tested substances, except ethanol, reduced POx comparable to intact skin levels. Therefore, the combined application of chitosan and diosgenin is a very promising and effective medication for wound healing. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T07:21:20Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2d68baa5bdae4e1fb40e814b8d9f37902023-11-17T07:57:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01245504910.3390/ijms24055049Effect of Chitosan-Diosgenin Combination on Wound HealingLubomir Petrov0Olya Stoilova1Georgi Pramatarov2Hristiyana Kanzova3Elina Tsvetanova4Madlena Andreeva5Almira Georgieva6Dimitrinka Atanasova7Stanislav Philipov8Albena Alexandrova9National Sports Academy “Vassil Levski”, 21 Akad. Stefan Mladenov St., Studentski Grad, 1700 Sofia, BulgariaLaboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Akad. G. Bonchev St., bl. 103A, 1113 Sofia, BulgariaLaboratory of Free Radical Processes, Institute of Neurobiology, Bulgarian Academy of Sciences, 23 Akad. G. Bonchev St., 1113 Sofia, BulgariaLaboratory of Free Radical Processes, Institute of Neurobiology, Bulgarian Academy of Sciences, 23 Akad. G. Bonchev St., 1113 Sofia, BulgariaLaboratory of Free Radical Processes, Institute of Neurobiology, Bulgarian Academy of Sciences, 23 Akad. G. Bonchev St., 1113 Sofia, BulgariaLaboratory of Free Radical Processes, Institute of Neurobiology, Bulgarian Academy of Sciences, 23 Akad. G. Bonchev St., 1113 Sofia, BulgariaLaboratory of Free Radical Processes, Institute of Neurobiology, Bulgarian Academy of Sciences, 23 Akad. G. Bonchev St., 1113 Sofia, BulgariaDepartment of Synaptic Signaling and Communication, Institute of Neurobiology, Bulgarian Academy of Sciences, 23 Akad. G. Bonchev St., 1113 Sofia, BulgariaDepartment of Anatomy, Histology, General and Clinical Pathology and Forensic Medicine, Faculty of Medicine, Sofia University St. Kl. Ohridski, Lozenets University Hospital, 1 Koziak St., 1000 Sofia, BulgariaNational Sports Academy “Vassil Levski”, 21 Akad. Stefan Mladenov St., Studentski Grad, 1700 Sofia, BulgariaThe difficult-to-heal wounds continue to be a problem for modern medicine. Chitosan and diosgenin possess anti-inflammatory and antioxidant effects making them relevant substances for wound treatment. That is why this work aimed to study the effect of the combined application of chitosan and diosgenin on a mouse skin wound model. For the purpose, wounds (6 mm diameter) were made on mice’s backs and were treated for 9 days with one of the following: 50% ethanol (control), polyethylene glycol (PEG) in 50% ethanol, chitosan and PEG in 50% ethanol (Chs), diosgenin and PEG in 50% ethanol (Dg) and chitosan, diosgenin and PEG in 50% ethanol (ChsDg). Before the first treatment and on the 3rd, 6th and 9th days, the wounds were photographed and their area was determined. On the 9th day, animals were euthanized and wounds’ tissues were excised for histological analysis. In addition, the lipid peroxidation (LPO), protein oxidation (POx) and total glutathione (tGSH) levels were measured. The results showed that ChsDg had the most pronounced overall effect on wound area reduction, followed by Chs and PEG. Moreover, the application of ChsDg maintained high levels of tGSH in wound tissues, compared to other substances. It was shown that all tested substances, except ethanol, reduced POx comparable to intact skin levels. Therefore, the combined application of chitosan and diosgenin is a very promising and effective medication for wound healing.https://www.mdpi.com/1422-0067/24/5/5049chitosandiosgeninmurine excisional wound modeloxidative stress |
| spellingShingle | Lubomir Petrov Olya Stoilova Georgi Pramatarov Hristiyana Kanzova Elina Tsvetanova Madlena Andreeva Almira Georgieva Dimitrinka Atanasova Stanislav Philipov Albena Alexandrova Effect of Chitosan-Diosgenin Combination on Wound Healing International Journal of Molecular Sciences chitosan diosgenin murine excisional wound model oxidative stress |
| title | Effect of Chitosan-Diosgenin Combination on Wound Healing |
| title_full | Effect of Chitosan-Diosgenin Combination on Wound Healing |
| title_fullStr | Effect of Chitosan-Diosgenin Combination on Wound Healing |
| title_full_unstemmed | Effect of Chitosan-Diosgenin Combination on Wound Healing |
| title_short | Effect of Chitosan-Diosgenin Combination on Wound Healing |
| title_sort | effect of chitosan diosgenin combination on wound healing |
| topic | chitosan diosgenin murine excisional wound model oxidative stress |
| url | https://www.mdpi.com/1422-0067/24/5/5049 |
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