Chemical Composition of Pinus roxburghii Bark Volatile Oil and Validation of Its Anti-Inflammatory Activity Using Molecular Modelling and Bleomycin-Induced Inflammation in Albino Mice
The chemical composition of Pinus roxburghii bark essential oil (PRO) was qualitatively and quantitatively determined using GC/FID and GC/MS. The anti-inflammatory activity was assessed in vitro by evaluating the binding percentages on the cannabinoids and opioids receptors. Bleomycin (BLM)-induced...
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MDPI AG
2017-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/22/9/1384 |
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| author | Rola M. Labib Fadia S. Youssef Mohamed L. Ashour Mohamed M. Abdel-Daim Samir A. Ross |
| author_facet | Rola M. Labib Fadia S. Youssef Mohamed L. Ashour Mohamed M. Abdel-Daim Samir A. Ross |
| author_sort | Rola M. Labib |
| collection | DOAJ |
| description | The chemical composition of Pinus roxburghii bark essential oil (PRO) was qualitatively and quantitatively determined using GC/FID and GC/MS. The anti-inflammatory activity was assessed in vitro by evaluating the binding percentages on the cannabinoids and opioids receptors. Bleomycin (BLM)-induced pulmonary inflammation in albino mice was adopted to assess PRO anti-inflammatory efficacy in vivo. In silico molecular modelling of its major components was performed on human glucocorticoids receptor (GR). Seventy-five components were identified in which longifolene (33.13%) and palmitic acid (9.34%) constituted the predominant components. No binding was observed on cannabinoid receptor type 1 (CB1), whereas mild binding was observed on cannabinoid receptor type 2 (CB2), delta, kappa, and mu receptors accounting for 2.9%, 6.9%, 10.9% and 22% binding. A significant in vivo activity was evidenced by reduction of the elevated malondialdehyde (MDA), nitric oxide (NO), myeloperoxidase (MPO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) levels by 55.56%, 55.66%, 64.64%, 58.85% and 77.78% with concomitant elevation of superoxide dismutase (SOD) and catalase (CAT) activities comparable to BLM-treated group at 100 mg/kg body weight. In silico studies showed that palmitic acid exerted the fittest binding. PRO could serve as a potent anti-inflammatory natural candidate that should be supported by further clinical trials. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T03:06:15Z |
| publishDate | 2017-08-01 |
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| series | Molecules |
| spelling | doaj.art-2d6a024c3a5246d09cffd7ec3a4269952022-12-22T03:05:13ZengMDPI AGMolecules1420-30492017-08-01229138410.3390/molecules22091384molecules22091384Chemical Composition of Pinus roxburghii Bark Volatile Oil and Validation of Its Anti-Inflammatory Activity Using Molecular Modelling and Bleomycin-Induced Inflammation in Albino MiceRola M. Labib0Fadia S. Youssef1Mohamed L. Ashour2Mohamed M. Abdel-Daim3Samir A. Ross4Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo 11566, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo 11566, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo 11566, EgyptDepartment of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptNational Center for Natural Products Research, University of Mississippi, University, MS 38677, USAThe chemical composition of Pinus roxburghii bark essential oil (PRO) was qualitatively and quantitatively determined using GC/FID and GC/MS. The anti-inflammatory activity was assessed in vitro by evaluating the binding percentages on the cannabinoids and opioids receptors. Bleomycin (BLM)-induced pulmonary inflammation in albino mice was adopted to assess PRO anti-inflammatory efficacy in vivo. In silico molecular modelling of its major components was performed on human glucocorticoids receptor (GR). Seventy-five components were identified in which longifolene (33.13%) and palmitic acid (9.34%) constituted the predominant components. No binding was observed on cannabinoid receptor type 1 (CB1), whereas mild binding was observed on cannabinoid receptor type 2 (CB2), delta, kappa, and mu receptors accounting for 2.9%, 6.9%, 10.9% and 22% binding. A significant in vivo activity was evidenced by reduction of the elevated malondialdehyde (MDA), nitric oxide (NO), myeloperoxidase (MPO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) levels by 55.56%, 55.66%, 64.64%, 58.85% and 77.78% with concomitant elevation of superoxide dismutase (SOD) and catalase (CAT) activities comparable to BLM-treated group at 100 mg/kg body weight. In silico studies showed that palmitic acid exerted the fittest binding. PRO could serve as a potent anti-inflammatory natural candidate that should be supported by further clinical trials.https://www.mdpi.com/1420-3049/22/9/1384anti-inflammatory activitybleomycingas-chromatographymolecular modellingPinus roxburghiiPinaceae discipline |
| spellingShingle | Rola M. Labib Fadia S. Youssef Mohamed L. Ashour Mohamed M. Abdel-Daim Samir A. Ross Chemical Composition of Pinus roxburghii Bark Volatile Oil and Validation of Its Anti-Inflammatory Activity Using Molecular Modelling and Bleomycin-Induced Inflammation in Albino Mice Molecules anti-inflammatory activity bleomycin gas-chromatography molecular modelling Pinus roxburghii Pinaceae discipline |
| title | Chemical Composition of Pinus roxburghii Bark Volatile Oil and Validation of Its Anti-Inflammatory Activity Using Molecular Modelling and Bleomycin-Induced Inflammation in Albino Mice |
| title_full | Chemical Composition of Pinus roxburghii Bark Volatile Oil and Validation of Its Anti-Inflammatory Activity Using Molecular Modelling and Bleomycin-Induced Inflammation in Albino Mice |
| title_fullStr | Chemical Composition of Pinus roxburghii Bark Volatile Oil and Validation of Its Anti-Inflammatory Activity Using Molecular Modelling and Bleomycin-Induced Inflammation in Albino Mice |
| title_full_unstemmed | Chemical Composition of Pinus roxburghii Bark Volatile Oil and Validation of Its Anti-Inflammatory Activity Using Molecular Modelling and Bleomycin-Induced Inflammation in Albino Mice |
| title_short | Chemical Composition of Pinus roxburghii Bark Volatile Oil and Validation of Its Anti-Inflammatory Activity Using Molecular Modelling and Bleomycin-Induced Inflammation in Albino Mice |
| title_sort | chemical composition of pinus roxburghii bark volatile oil and validation of its anti inflammatory activity using molecular modelling and bleomycin induced inflammation in albino mice |
| topic | anti-inflammatory activity bleomycin gas-chromatography molecular modelling Pinus roxburghii Pinaceae discipline |
| url | https://www.mdpi.com/1420-3049/22/9/1384 |
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