Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiation
In this study, fibroblasts were harvested and isolated from a healthy 14-year-old male donor and reprogrammed with four Yamanaka factors containing Oct3/4, Sox2, Klf4 and c-Myc to generate human induced pluripotent stem cell (iPSC) lines. The resulting iPSCs were integration-free, expressed normal k...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Stem Cell Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506123001873 |
| _version_ | 1797642557511958528 |
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| author | Yunfei Zhang Yali Liu Wei Yan Xuecong Zhang Yu Hou Zijing Zhou Ruiyun Guo Guobin Liu Jun Ma |
| author_facet | Yunfei Zhang Yali Liu Wei Yan Xuecong Zhang Yu Hou Zijing Zhou Ruiyun Guo Guobin Liu Jun Ma |
| author_sort | Yunfei Zhang |
| collection | DOAJ |
| description | In this study, fibroblasts were harvested and isolated from a healthy 14-year-old male donor and reprogrammed with four Yamanaka factors containing Oct3/4, Sox2, Klf4 and c-Myc to generate human induced pluripotent stem cell (iPSC) lines. The resulting iPSCs were integration-free, expressed normal karyotype, displayed pluripotency markers, and have been demonstrated to differentiate into cells with three germ layer. And the iPSCs were further differentiated to chondrosphere in vivo. The models could be used to test multiple differentiation protocols and also as a control for screening drugs and studying cartilage related disease. |
| first_indexed | 2024-03-11T14:01:56Z |
| format | Article |
| id | doaj.art-2d6a03d43aaa402da7e7bae65c30c517 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-11T14:01:56Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-2d6a03d43aaa402da7e7bae65c30c5172023-11-02T04:13:27ZengElsevierStem Cell Research1873-50612023-10-0172103201Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiationYunfei Zhang0Yali Liu1Wei Yan2Xuecong Zhang3Yu Hou4Zijing Zhou5Ruiyun Guo6Guobin Liu7Jun Ma8Emergency Department, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province 050000, China; Hebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaChild Health (Psychological Behavior) Department, Hebei Children's Hospital, Hebei Province 050017, ChinaHebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaHebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaHebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaHebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaHebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaThe First Hospital of Hebei Medical University, Hebei Province 050000, China; Corresponding authors at: The First Hospital of Hebei Medical University, Hebei Province 050000, China (G. Liu); Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Medical University, Hebei Province 050017, China (J. Ma).Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Child Health (Psychological Behavior) Department, Hebei Children's Hospital, Hebei Province 050017, China; Corresponding authors at: The First Hospital of Hebei Medical University, Hebei Province 050000, China (G. Liu); Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Medical University, Hebei Province 050017, China (J. Ma).In this study, fibroblasts were harvested and isolated from a healthy 14-year-old male donor and reprogrammed with four Yamanaka factors containing Oct3/4, Sox2, Klf4 and c-Myc to generate human induced pluripotent stem cell (iPSC) lines. The resulting iPSCs were integration-free, expressed normal karyotype, displayed pluripotency markers, and have been demonstrated to differentiate into cells with three germ layer. And the iPSCs were further differentiated to chondrosphere in vivo. The models could be used to test multiple differentiation protocols and also as a control for screening drugs and studying cartilage related disease.http://www.sciencedirect.com/science/article/pii/S1873506123001873Induced pluripotent stem cellChondrogenic differentiationReprogrammingCartilage |
| spellingShingle | Yunfei Zhang Yali Liu Wei Yan Xuecong Zhang Yu Hou Zijing Zhou Ruiyun Guo Guobin Liu Jun Ma Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiation Stem Cell Research Induced pluripotent stem cell Chondrogenic differentiation Reprogramming Cartilage |
| title | Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiation |
| title_full | Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiation |
| title_fullStr | Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiation |
| title_full_unstemmed | Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiation |
| title_short | Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiation |
| title_sort | human induced pluripotent stem cell line from fibroblasts hebhmui015 a and chondrogenic differentiation |
| topic | Induced pluripotent stem cell Chondrogenic differentiation Reprogramming Cartilage |
| url | http://www.sciencedirect.com/science/article/pii/S1873506123001873 |
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