Laboratory-Controlled Experiments Reveal Microbial Community Shifts during Sediment Resuspension Events
In freshwater ecosystems, dynamic hydraulic events (floods or dam maintenance) lead to sediment resuspension and mixing with waters of different composition. Microbial communities living in the sediments play a major role in these leaching events, contributing to organic matter degradation and the r...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/8/1416 |
| _version_ | 1797410012763521024 |
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| author | Alexis DesRosiers Nathalie Gassama Cécile Grosbois Cassandre Sara Lazar |
| author_facet | Alexis DesRosiers Nathalie Gassama Cécile Grosbois Cassandre Sara Lazar |
| author_sort | Alexis DesRosiers |
| collection | DOAJ |
| description | In freshwater ecosystems, dynamic hydraulic events (floods or dam maintenance) lead to sediment resuspension and mixing with waters of different composition. Microbial communities living in the sediments play a major role in these leaching events, contributing to organic matter degradation and the release of trace elements. However, the dynamics of community diversity are seldom studied in the context of ecological studies. Therefore, we carried out laboratory-induced leaching experiments, using sediments from the Villerest dam reservoir (Villerest, France). To assess whole microbial community diversity, we sequenced the archaeal and bacterial 16S rRNA genes using Illumina MiSeq. Our results suggest that the degree of dissolved oxygen found in the water during these resuspension episodes influenced community dynamics, with anoxic waters leading to drastic shifts in sedimentary communities compared to oxic waters. Furthermore, the release of microbial cells from sediments to the water column were more favorable to water colonization when events were caused by oxic waters. Most of the bacteria found in the sediments were chemoorganotrophs and most of the archaea were methanogens. Methylotrophic, as well as archaeal, and bacterial chemoorganotrophs were detected in the leachate samples. These results also show that organic matter degradation occurred, likely participating in carbonate dissolution and the release of trace elements during freshwater resuspension events. |
| first_indexed | 2024-03-09T04:23:42Z |
| format | Article |
| id | doaj.art-2d6bd117d6a74a8087f38f4bc6bff0a6 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T04:23:42Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-2d6bd117d6a74a8087f38f4bc6bff0a62023-12-03T13:43:06ZengMDPI AGGenes2073-44252022-08-01138141610.3390/genes13081416Laboratory-Controlled Experiments Reveal Microbial Community Shifts during Sediment Resuspension EventsAlexis DesRosiers0Nathalie Gassama1Cécile Grosbois2Cassandre Sara Lazar3Department of Biological Sciences, University of Québec at Montréal, UQAM, 141 Avenue du Président-Kennedy, Montreal, QC H2X 1Y4, CanadaGéHCO Géo-Hydrosystèmes Continentaux, Université de Tours, 37200 Tours, FranceGéHCO Géo-Hydrosystèmes Continentaux, Université de Tours, 37200 Tours, FranceDepartment of Biological Sciences, University of Québec at Montréal, UQAM, 141 Avenue du Président-Kennedy, Montreal, QC H2X 1Y4, CanadaIn freshwater ecosystems, dynamic hydraulic events (floods or dam maintenance) lead to sediment resuspension and mixing with waters of different composition. Microbial communities living in the sediments play a major role in these leaching events, contributing to organic matter degradation and the release of trace elements. However, the dynamics of community diversity are seldom studied in the context of ecological studies. Therefore, we carried out laboratory-induced leaching experiments, using sediments from the Villerest dam reservoir (Villerest, France). To assess whole microbial community diversity, we sequenced the archaeal and bacterial 16S rRNA genes using Illumina MiSeq. Our results suggest that the degree of dissolved oxygen found in the water during these resuspension episodes influenced community dynamics, with anoxic waters leading to drastic shifts in sedimentary communities compared to oxic waters. Furthermore, the release of microbial cells from sediments to the water column were more favorable to water colonization when events were caused by oxic waters. Most of the bacteria found in the sediments were chemoorganotrophs and most of the archaea were methanogens. Methylotrophic, as well as archaeal, and bacterial chemoorganotrophs were detected in the leachate samples. These results also show that organic matter degradation occurred, likely participating in carbonate dissolution and the release of trace elements during freshwater resuspension events.https://www.mdpi.com/2073-4425/13/8/1416dam freshwaterdam sedimentsediment leachingarchaeabacteria |
| spellingShingle | Alexis DesRosiers Nathalie Gassama Cécile Grosbois Cassandre Sara Lazar Laboratory-Controlled Experiments Reveal Microbial Community Shifts during Sediment Resuspension Events Genes dam freshwater dam sediment sediment leaching archaea bacteria |
| title | Laboratory-Controlled Experiments Reveal Microbial Community Shifts during Sediment Resuspension Events |
| title_full | Laboratory-Controlled Experiments Reveal Microbial Community Shifts during Sediment Resuspension Events |
| title_fullStr | Laboratory-Controlled Experiments Reveal Microbial Community Shifts during Sediment Resuspension Events |
| title_full_unstemmed | Laboratory-Controlled Experiments Reveal Microbial Community Shifts during Sediment Resuspension Events |
| title_short | Laboratory-Controlled Experiments Reveal Microbial Community Shifts during Sediment Resuspension Events |
| title_sort | laboratory controlled experiments reveal microbial community shifts during sediment resuspension events |
| topic | dam freshwater dam sediment sediment leaching archaea bacteria |
| url | https://www.mdpi.com/2073-4425/13/8/1416 |
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