Cemp1-p3 Peptide Promotes the Transformation of Octacalcium Phosphate into Hydroxyapatite Crystals
Dental cementum contains unique molecules that regulate the mineralization process in vitro and in vivo, such as cementum protein 1 (CEMP1). This protein possesses amino acid sequence motifs like the human recombinant CEMP1 with biological activity. This novel cementum protein 1-derived peptide (CEM...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Crystals |
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| Online Access: | https://www.mdpi.com/2073-4352/10/12/1131 |
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| author | Maricela Santana Gonzalo Montoya Raúl Herrera Lía Hoz Enrique Romo Claudia Zamora Ana Wintergerst Higinio Arzate |
| author_facet | Maricela Santana Gonzalo Montoya Raúl Herrera Lía Hoz Enrique Romo Claudia Zamora Ana Wintergerst Higinio Arzate |
| author_sort | Maricela Santana |
| collection | DOAJ |
| description | Dental cementum contains unique molecules that regulate the mineralization process in vitro and in vivo, such as cementum protein 1 (CEMP1). This protein possesses amino acid sequence motifs like the human recombinant CEMP1 with biological activity. This novel cementum protein 1-derived peptide (CEMP1-p3, from the CEMP1’s N-terminal domain: (QPLPKGCAAVKAEVGIPAPH), consists of 20 amino acids. Hydroxyapatite (HA) crystals could be obtained through the combination of the amorphous precursor phase and macromolecules such as proteins and peptides. We used a simple method to synthesize peptide/hydroxyapatite nanocomposites using OCP and CEMP1-p3. The characterization of the crystals through scanning electron microscopy (SEM), powder X-ray diffraction (XRD), high--resolution transmission electron microscopy (HRTEM), and Raman spectroscopy revealed that CEMP1-p3 transformed OCP into hydroxyapatite (HA) under constant ionic strength and in a buffered solution. CEMP1-p3 binds and highly adsorbs to OCP and is a potent growth stimulator of OCP crystals. CEMP1-p3 fosters the transformation of OCP into HA crystals with crystalline planes (300) and (004) that correspond to the cell of hexagonal HA. Octacalcium phosphate crystals treated with CEMP1-p3 grown in simulated physiological buffer acquired hexagonal arrangement corresponding to HA. These findings provide new insights into the potential application of CEMP1-p3 on possible biomimetic approaches to generate materials for the repair and regeneration of mineralized tissues, or restorative materials in the orthopedic field. |
| first_indexed | 2024-03-10T14:08:35Z |
| format | Article |
| id | doaj.art-2d6dcb8e535244738cac6a01ef71496c |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-03-10T14:08:35Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-2d6dcb8e535244738cac6a01ef71496c2023-11-21T00:23:58ZengMDPI AGCrystals2073-43522020-12-011012113110.3390/cryst10121131Cemp1-p3 Peptide Promotes the Transformation of Octacalcium Phosphate into Hydroxyapatite CrystalsMaricela Santana0Gonzalo Montoya1Raúl Herrera2Lía Hoz3Enrique Romo4Claudia Zamora5Ana Wintergerst6Higinio Arzate7Laboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoLaboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoInstituto de Física, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoLaboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoLaboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoLaboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoLaboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoLaboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoDental cementum contains unique molecules that regulate the mineralization process in vitro and in vivo, such as cementum protein 1 (CEMP1). This protein possesses amino acid sequence motifs like the human recombinant CEMP1 with biological activity. This novel cementum protein 1-derived peptide (CEMP1-p3, from the CEMP1’s N-terminal domain: (QPLPKGCAAVKAEVGIPAPH), consists of 20 amino acids. Hydroxyapatite (HA) crystals could be obtained through the combination of the amorphous precursor phase and macromolecules such as proteins and peptides. We used a simple method to synthesize peptide/hydroxyapatite nanocomposites using OCP and CEMP1-p3. The characterization of the crystals through scanning electron microscopy (SEM), powder X-ray diffraction (XRD), high--resolution transmission electron microscopy (HRTEM), and Raman spectroscopy revealed that CEMP1-p3 transformed OCP into hydroxyapatite (HA) under constant ionic strength and in a buffered solution. CEMP1-p3 binds and highly adsorbs to OCP and is a potent growth stimulator of OCP crystals. CEMP1-p3 fosters the transformation of OCP into HA crystals with crystalline planes (300) and (004) that correspond to the cell of hexagonal HA. Octacalcium phosphate crystals treated with CEMP1-p3 grown in simulated physiological buffer acquired hexagonal arrangement corresponding to HA. These findings provide new insights into the potential application of CEMP1-p3 on possible biomimetic approaches to generate materials for the repair and regeneration of mineralized tissues, or restorative materials in the orthopedic field.https://www.mdpi.com/2073-4352/10/12/1131crystal growthoctacalcium phosphatehydroxyapatitecementum protein 1 (CEMP1)peptidesmineralization |
| spellingShingle | Maricela Santana Gonzalo Montoya Raúl Herrera Lía Hoz Enrique Romo Claudia Zamora Ana Wintergerst Higinio Arzate Cemp1-p3 Peptide Promotes the Transformation of Octacalcium Phosphate into Hydroxyapatite Crystals Crystals crystal growth octacalcium phosphate hydroxyapatite cementum protein 1 (CEMP1) peptides mineralization |
| title | Cemp1-p3 Peptide Promotes the Transformation of Octacalcium Phosphate into Hydroxyapatite Crystals |
| title_full | Cemp1-p3 Peptide Promotes the Transformation of Octacalcium Phosphate into Hydroxyapatite Crystals |
| title_fullStr | Cemp1-p3 Peptide Promotes the Transformation of Octacalcium Phosphate into Hydroxyapatite Crystals |
| title_full_unstemmed | Cemp1-p3 Peptide Promotes the Transformation of Octacalcium Phosphate into Hydroxyapatite Crystals |
| title_short | Cemp1-p3 Peptide Promotes the Transformation of Octacalcium Phosphate into Hydroxyapatite Crystals |
| title_sort | cemp1 p3 peptide promotes the transformation of octacalcium phosphate into hydroxyapatite crystals |
| topic | crystal growth octacalcium phosphate hydroxyapatite cementum protein 1 (CEMP1) peptides mineralization |
| url | https://www.mdpi.com/2073-4352/10/12/1131 |
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