Genetic Diversity of <i>Babesia canis</i> Strains in Dogs in Lithuania
Canine babesiosis is an emerging and rapidly expanding tick-borne disease in central and northeast Europe. In the last two decades, the endemic area of <i>Babesia canis</i> has expanded from central Europe to the Baltic region. This study aimed to investigate the genetic diversity of <...
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MDPI AG
2022-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/7/1446 |
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| author | Jana Radzijevskaja Dalytė Mardosaitė-Busaitienė Asta Aleksandravičienė Birutė Karvelienė Miglė Razgūnaitė Inga Stadalienė Algimantas Paulauskas |
| author_facet | Jana Radzijevskaja Dalytė Mardosaitė-Busaitienė Asta Aleksandravičienė Birutė Karvelienė Miglė Razgūnaitė Inga Stadalienė Algimantas Paulauskas |
| author_sort | Jana Radzijevskaja |
| collection | DOAJ |
| description | Canine babesiosis is an emerging and rapidly expanding tick-borne disease in central and northeast Europe. In the last two decades, the endemic area of <i>Babesia canis</i> has expanded from central Europe to the Baltic region. This study aimed to investigate the genetic diversity of <i>B. canis</i> strains isolated from naturally infected dogs in different regions of Lithuania using PCR-RFLP and sequence analyses based on a partial region of 18S rRNA and <i>Bc28.1</i> genes. Blood samples from 149 dogs suspected of having babesiosis were collected in Lithuania during 2016–2017. Based on PCR-RFLP profiles and two nucleotide substitutions observed in 18S rRNA gene sequences, three <i>B. canis</i> genotypes were identified in Lithuania—18S rRNA-A, 18S rRNA-B and 18S rRNA-A/B—with the A/B genotype predominating (83.9%). Based on the obtained PCR-RFLP profiles of the Bc28.1 gene, four <i>B. canis</i> genotypes were identified: Bc28.1-B (53.8%), Bc28.1-34 (20.8%), Bc28.1-A (17.9%), and Bc28.1-34/A or B (7.5%). Sequence analysis of the partial <i>Bc28.1</i> gene revealed eighteen polymorphic sites and thirteen sequence variants among the Lithuanian samples. The <i>B. canis</i> genotypes obtained were detected with varying prevalences in different regions of Lithuania. |
| first_indexed | 2024-03-09T06:10:10Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T06:10:10Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-2d6e4dfccc8d4717aaf4122eb12630712023-12-03T11:59:31ZengMDPI AGMicroorganisms2076-26072022-07-01107144610.3390/microorganisms10071446Genetic Diversity of <i>Babesia canis</i> Strains in Dogs in LithuaniaJana Radzijevskaja0Dalytė Mardosaitė-Busaitienė1Asta Aleksandravičienė2Birutė Karvelienė3Miglė Razgūnaitė4Inga Stadalienė5Algimantas Paulauskas6Faculty of Natural Sciences, Vytautas Magnus University, K. Donelaičio Str. 58, LT-44248 Kaunas, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, K. Donelaičio Str. 58, LT-44248 Kaunas, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, K. Donelaičio Str. 58, LT-44248 Kaunas, LithuaniaFaculty of Veterinary Medicine, Lithuanian University of Health Sciences, Tilžės Str. 18, LT-47181 Kaunas, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, K. Donelaičio Str. 58, LT-44248 Kaunas, LithuaniaFaculty of Veterinary Medicine, Lithuanian University of Health Sciences, Tilžės Str. 18, LT-47181 Kaunas, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, K. Donelaičio Str. 58, LT-44248 Kaunas, LithuaniaCanine babesiosis is an emerging and rapidly expanding tick-borne disease in central and northeast Europe. In the last two decades, the endemic area of <i>Babesia canis</i> has expanded from central Europe to the Baltic region. This study aimed to investigate the genetic diversity of <i>B. canis</i> strains isolated from naturally infected dogs in different regions of Lithuania using PCR-RFLP and sequence analyses based on a partial region of 18S rRNA and <i>Bc28.1</i> genes. Blood samples from 149 dogs suspected of having babesiosis were collected in Lithuania during 2016–2017. Based on PCR-RFLP profiles and two nucleotide substitutions observed in 18S rRNA gene sequences, three <i>B. canis</i> genotypes were identified in Lithuania—18S rRNA-A, 18S rRNA-B and 18S rRNA-A/B—with the A/B genotype predominating (83.9%). Based on the obtained PCR-RFLP profiles of the Bc28.1 gene, four <i>B. canis</i> genotypes were identified: Bc28.1-B (53.8%), Bc28.1-34 (20.8%), Bc28.1-A (17.9%), and Bc28.1-34/A or B (7.5%). Sequence analysis of the partial <i>Bc28.1</i> gene revealed eighteen polymorphic sites and thirteen sequence variants among the Lithuanian samples. The <i>B. canis</i> genotypes obtained were detected with varying prevalences in different regions of Lithuania.https://www.mdpi.com/2076-2607/10/7/1446<i>Babesia canis</i>canine babesiosisPCR-RFLP18S rRNABc28.1Lithuania |
| spellingShingle | Jana Radzijevskaja Dalytė Mardosaitė-Busaitienė Asta Aleksandravičienė Birutė Karvelienė Miglė Razgūnaitė Inga Stadalienė Algimantas Paulauskas Genetic Diversity of <i>Babesia canis</i> Strains in Dogs in Lithuania Microorganisms <i>Babesia canis</i> canine babesiosis PCR-RFLP 18S rRNA Bc28.1 Lithuania |
| title | Genetic Diversity of <i>Babesia canis</i> Strains in Dogs in Lithuania |
| title_full | Genetic Diversity of <i>Babesia canis</i> Strains in Dogs in Lithuania |
| title_fullStr | Genetic Diversity of <i>Babesia canis</i> Strains in Dogs in Lithuania |
| title_full_unstemmed | Genetic Diversity of <i>Babesia canis</i> Strains in Dogs in Lithuania |
| title_short | Genetic Diversity of <i>Babesia canis</i> Strains in Dogs in Lithuania |
| title_sort | genetic diversity of i babesia canis i strains in dogs in lithuania |
| topic | <i>Babesia canis</i> canine babesiosis PCR-RFLP 18S rRNA Bc28.1 Lithuania |
| url | https://www.mdpi.com/2076-2607/10/7/1446 |
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