Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta
Preeclampsia is a human pregnancy-specific disease characterized by abnormal placentation that usually presents with maternal hypertension and proteinuria. The main hallmark of preeclampsia, impaired trophoblast migration, and the subsequent disruption of uterine arteries remodeling lead to several...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| author | Marta Lombó Christian Giommi Michela Paolucci Valentina Notarstefano Nina Montik Giovanni Delli Carpini Andrea Ciavattini Antonio Ragusa Francesca Maradonna Elisabetta Giorgini Oliana Carnevali |
| author_facet | Marta Lombó Christian Giommi Michela Paolucci Valentina Notarstefano Nina Montik Giovanni Delli Carpini Andrea Ciavattini Antonio Ragusa Francesca Maradonna Elisabetta Giorgini Oliana Carnevali |
| author_sort | Marta Lombó |
| collection | DOAJ |
| description | Preeclampsia is a human pregnancy-specific disease characterized by abnormal placentation that usually presents with maternal hypertension and proteinuria. The main hallmark of preeclampsia, impaired trophoblast migration, and the subsequent disruption of uterine arteries remodeling lead to several molecular alterations in the placental compartments with those occurring in the chorionic villi being of the utmost importance. Given the essential role of the endocannabinoid system during preimplantation and trophoblast migration, we have combined the histological and hyperspectral imaging analyses to shed light on the involvement of two cannabinoid receptors in the macromolecular alterations related to preeclampsia. The results obtained by immunohistochemistry showed a significant increase in the protein levels of cannabinoid receptor 1 (CB1) in the preeclamptic chorionic villi. However, no changes were reported regarding transient receptor potential vanilloid 1 (TRPV-1) levels either in the bulk placental samples or chorionic villi when comparing control and preeclamptic patients. Histological analysis and Fourier-transform infrared spectroscopy (FTIRI) showed an increase in collagen deposition together with higher levels of lipid peroxidation and phosphorylated compounds in the pathological villi. Since CB1 enhancement has been described as promoting fibrosis and oxidative stress in several tissues, we proposed that the higher receptor abundance in preeclampsia could be triggering similar molecular effects in preeclamptic term placentas. |
| first_indexed | 2024-03-09T19:01:21Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:01:21Z |
| publishDate | 2022-10-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2d7154c091aa44df85c39ec9052e06792023-11-24T04:59:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123211293110.3390/ijms232112931Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term PlacentaMarta Lombó0Christian Giommi1Michela Paolucci2Valentina Notarstefano3Nina Montik4Giovanni Delli Carpini5Andrea Ciavattini6Antonio Ragusa7Francesca Maradonna8Elisabetta Giorgini9Oliana Carnevali10Department of Life and Environmental Sciences, Università Politecnica delle Marche, 60131 Ancona, ItalyDepartment of Life and Environmental Sciences, Università Politecnica delle Marche, 60131 Ancona, ItalyDepartment of Odontostomatological and Specialized Clinical Sciences, Università Politecnica delle Marche, 60020 Ancona, ItalyDepartment of Life and Environmental Sciences, Università Politecnica delle Marche, 60131 Ancona, ItalyDepartment of Odontostomatological and Specialized Clinical Sciences, Università Politecnica delle Marche, 60020 Ancona, ItalyDepartment of Odontostomatological and Specialized Clinical Sciences, Università Politecnica delle Marche, 60020 Ancona, ItalyDepartment of Odontostomatological and Specialized Clinical Sciences, Università Politecnica delle Marche, 60020 Ancona, ItalyDepartment of Obstetrics and Gynecology, Università Campus Bio Medico di Roma, 00128 Roma, ItalyDepartment of Life and Environmental Sciences, Università Politecnica delle Marche, 60131 Ancona, ItalyDepartment of Life and Environmental Sciences, Università Politecnica delle Marche, 60131 Ancona, ItalyDepartment of Life and Environmental Sciences, Università Politecnica delle Marche, 60131 Ancona, ItalyPreeclampsia is a human pregnancy-specific disease characterized by abnormal placentation that usually presents with maternal hypertension and proteinuria. The main hallmark of preeclampsia, impaired trophoblast migration, and the subsequent disruption of uterine arteries remodeling lead to several molecular alterations in the placental compartments with those occurring in the chorionic villi being of the utmost importance. Given the essential role of the endocannabinoid system during preimplantation and trophoblast migration, we have combined the histological and hyperspectral imaging analyses to shed light on the involvement of two cannabinoid receptors in the macromolecular alterations related to preeclampsia. The results obtained by immunohistochemistry showed a significant increase in the protein levels of cannabinoid receptor 1 (CB1) in the preeclamptic chorionic villi. However, no changes were reported regarding transient receptor potential vanilloid 1 (TRPV-1) levels either in the bulk placental samples or chorionic villi when comparing control and preeclamptic patients. Histological analysis and Fourier-transform infrared spectroscopy (FTIRI) showed an increase in collagen deposition together with higher levels of lipid peroxidation and phosphorylated compounds in the pathological villi. Since CB1 enhancement has been described as promoting fibrosis and oxidative stress in several tissues, we proposed that the higher receptor abundance in preeclampsia could be triggering similar molecular effects in preeclamptic term placentas.https://www.mdpi.com/1422-0067/23/21/12931preeclampsiaendocannabinoid systemCB1collagenlipid peroxidationFTIRI |
| spellingShingle | Marta Lombó Christian Giommi Michela Paolucci Valentina Notarstefano Nina Montik Giovanni Delli Carpini Andrea Ciavattini Antonio Ragusa Francesca Maradonna Elisabetta Giorgini Oliana Carnevali Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta International Journal of Molecular Sciences preeclampsia endocannabinoid system CB1 collagen lipid peroxidation FTIRI |
| title | Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta |
| title_full | Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta |
| title_fullStr | Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta |
| title_full_unstemmed | Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta |
| title_short | Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta |
| title_sort | preeclampsia correlates with an increase in cannabinoid receptor 1 levels leading to macromolecular alterations in chorionic villi of term placenta |
| topic | preeclampsia endocannabinoid system CB1 collagen lipid peroxidation FTIRI |
| url | https://www.mdpi.com/1422-0067/23/21/12931 |
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