Evaluation of the antibacterial activity of Weissella confusa K3 cell-free supernatant against extended-spectrum βeta lactamase (ESBL) producing uropathogenic Escherichia coli U60
Different strategies have been approved for controlling extended-spectrum βeta lactamase (ESBL) producing uropathogenic bacteria. The antibacterial activity of Lactic acid bacteria (LAB) is an effective strategy due to its probiotic characteristics and beneficial effects on human health. The antibio...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Saudi Journal of Biological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319562X23000402 |
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| author | Rasha M. El-Mekkawy Noura E. Hamour Wesam A. Hassanein Ayman A. Allam |
| author_facet | Rasha M. El-Mekkawy Noura E. Hamour Wesam A. Hassanein Ayman A. Allam |
| author_sort | Rasha M. El-Mekkawy |
| collection | DOAJ |
| description | Different strategies have been approved for controlling extended-spectrum βeta lactamase (ESBL) producing uropathogenic bacteria. The antibacterial activity of Lactic acid bacteria (LAB) is an effective strategy due to its probiotic characteristics and beneficial effects on human health. The antibiotic susceptibility test, disk diffusion method, and double disc synergy test indicated that five enteric uropathogenic isolates were ESBL producers during the present study. They recorded diameters of inhibition zones as ≤ 18, ≤ 8, ≤ 19, and ≤ 8 mm against cefotaxime (CTX), ceftazidime (CAZ), aztreonam (ATM), and ceftriaxone (CRO). Genotypically, blaTEM genes are the most common, with (100 %) occurrence in all the five enteric tested uropathogens, followed by blaSHV and blaCTX genes (60 %). In addition, out of 10 LAB isolates from dairy products, the CFS of isolate no. K3 had high antibacterial activity against the tested ESBLs, especially no. U60, with a MIC of 600 µl. Additionally, the MIC and sub-MIC of K3 CFS inhibited the production of antibiotic-resistant bla TEM genes of U60. Analyzing the 16S rRNA sequence confirmed that the most potent ESBL-producing bacteria (U60) and LAB (K3) isolates were identified as Escherichia coli U60.1 and Weissella confuse K3 with accession numbers MW173246 and MW173299.1, respectively, in GenBank. |
| first_indexed | 2024-04-09T19:15:29Z |
| format | Article |
| id | doaj.art-2d727b561ea8428cb33294fe4c1091ba |
| institution | Directory Open Access Journal |
| issn | 1319-562X |
| language | English |
| last_indexed | 2024-04-09T19:15:29Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Journal of Biological Sciences |
| spelling | doaj.art-2d727b561ea8428cb33294fe4c1091ba2023-04-06T06:10:24ZengElsevierSaudi Journal of Biological Sciences1319-562X2023-04-01304103595Evaluation of the antibacterial activity of Weissella confusa K3 cell-free supernatant against extended-spectrum βeta lactamase (ESBL) producing uropathogenic Escherichia coli U60Rasha M. El-Mekkawy0Noura E. Hamour1Wesam A. Hassanein2Ayman A. Allam3Department of Botany and Microbiology, Faculty of Science, Zagazig University, 44519, Egypt; Corresponding author at: Department of Botany and Microbiology, Faculty of Science, Zagazig University, Sharqia Governorate, Zagazig 44519, Egypt.Department of Botany and Microbiology, Faculty of Science, Zagazig University, 44519, EgyptDepartment of Botany and Microbiology, Faculty of Science, Zagazig University, 44519, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, EgyptDifferent strategies have been approved for controlling extended-spectrum βeta lactamase (ESBL) producing uropathogenic bacteria. The antibacterial activity of Lactic acid bacteria (LAB) is an effective strategy due to its probiotic characteristics and beneficial effects on human health. The antibiotic susceptibility test, disk diffusion method, and double disc synergy test indicated that five enteric uropathogenic isolates were ESBL producers during the present study. They recorded diameters of inhibition zones as ≤ 18, ≤ 8, ≤ 19, and ≤ 8 mm against cefotaxime (CTX), ceftazidime (CAZ), aztreonam (ATM), and ceftriaxone (CRO). Genotypically, blaTEM genes are the most common, with (100 %) occurrence in all the five enteric tested uropathogens, followed by blaSHV and blaCTX genes (60 %). In addition, out of 10 LAB isolates from dairy products, the CFS of isolate no. K3 had high antibacterial activity against the tested ESBLs, especially no. U60, with a MIC of 600 µl. Additionally, the MIC and sub-MIC of K3 CFS inhibited the production of antibiotic-resistant bla TEM genes of U60. Analyzing the 16S rRNA sequence confirmed that the most potent ESBL-producing bacteria (U60) and LAB (K3) isolates were identified as Escherichia coli U60.1 and Weissella confuse K3 with accession numbers MW173246 and MW173299.1, respectively, in GenBank.http://www.sciencedirect.com/science/article/pii/S1319562X23000402Antibiotic resistanceLactic acid bacteriaAntibacterial activityExtended-spectrum beta-lactamaseUrinary tract infection |
| spellingShingle | Rasha M. El-Mekkawy Noura E. Hamour Wesam A. Hassanein Ayman A. Allam Evaluation of the antibacterial activity of Weissella confusa K3 cell-free supernatant against extended-spectrum βeta lactamase (ESBL) producing uropathogenic Escherichia coli U60 Saudi Journal of Biological Sciences Antibiotic resistance Lactic acid bacteria Antibacterial activity Extended-spectrum beta-lactamase Urinary tract infection |
| title | Evaluation of the antibacterial activity of Weissella confusa K3 cell-free supernatant against extended-spectrum βeta lactamase (ESBL) producing uropathogenic Escherichia coli U60 |
| title_full | Evaluation of the antibacterial activity of Weissella confusa K3 cell-free supernatant against extended-spectrum βeta lactamase (ESBL) producing uropathogenic Escherichia coli U60 |
| title_fullStr | Evaluation of the antibacterial activity of Weissella confusa K3 cell-free supernatant against extended-spectrum βeta lactamase (ESBL) producing uropathogenic Escherichia coli U60 |
| title_full_unstemmed | Evaluation of the antibacterial activity of Weissella confusa K3 cell-free supernatant against extended-spectrum βeta lactamase (ESBL) producing uropathogenic Escherichia coli U60 |
| title_short | Evaluation of the antibacterial activity of Weissella confusa K3 cell-free supernatant against extended-spectrum βeta lactamase (ESBL) producing uropathogenic Escherichia coli U60 |
| title_sort | evaluation of the antibacterial activity of weissella confusa k3 cell free supernatant against extended spectrum βeta lactamase esbl producing uropathogenic escherichia coli u60 |
| topic | Antibiotic resistance Lactic acid bacteria Antibacterial activity Extended-spectrum beta-lactamase Urinary tract infection |
| url | http://www.sciencedirect.com/science/article/pii/S1319562X23000402 |
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