Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae.
Lumpy skin disease (LSD) is an emerging disease of cattle in Kazakhstan and the means of transmission remains uncertain. In the current study, retention of Lumpy Skin Disease Virus (LSDV) by three Stomoxys species following intrathoracic inoculation was demonstrated under laboratory conditions. A vi...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0238210 |
| _version_ | 1818929602517532672 |
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| author | Arman Issimov David B Taylor Malik Shalmenov Birzhan Nurgaliyev Izimgali Zhubantayev Nurzhan Abekeshev Kaissar Kushaliyev Abzal Kereyev Lespek Kutumbetov Assylbek Zhanabayev Yasmin Zhakiyanova Peter J White |
| author_facet | Arman Issimov David B Taylor Malik Shalmenov Birzhan Nurgaliyev Izimgali Zhubantayev Nurzhan Abekeshev Kaissar Kushaliyev Abzal Kereyev Lespek Kutumbetov Assylbek Zhanabayev Yasmin Zhakiyanova Peter J White |
| author_sort | Arman Issimov |
| collection | DOAJ |
| description | Lumpy skin disease (LSD) is an emerging disease of cattle in Kazakhstan and the means of transmission remains uncertain. In the current study, retention of Lumpy Skin Disease Virus (LSDV) by three Stomoxys species following intrathoracic inoculation was demonstrated under laboratory conditions. A virulent LSDV strain was injected into the thorax of flies to bypass the midgut barrier. The fate of the pathogen in the hemolymph of the flies was examined using PCR and virus isolation tests. LSDV was isolated from all three Stomoxys species up to 24h post inoculation while virus DNA was detectable up to 7d post inoculation. |
| first_indexed | 2024-12-20T03:47:25Z |
| format | Article |
| id | doaj.art-2d73cef30ed54c4cb883f45d7370f578 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T03:47:25Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2d73cef30ed54c4cb883f45d7370f5782022-12-21T19:54:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01162e023821010.1371/journal.pone.0238210Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae.Arman IssimovDavid B TaylorMalik ShalmenovBirzhan NurgaliyevIzimgali ZhubantayevNurzhan AbekeshevKaissar KushaliyevAbzal KereyevLespek KutumbetovAssylbek ZhanabayevYasmin ZhakiyanovaPeter J WhiteLumpy skin disease (LSD) is an emerging disease of cattle in Kazakhstan and the means of transmission remains uncertain. In the current study, retention of Lumpy Skin Disease Virus (LSDV) by three Stomoxys species following intrathoracic inoculation was demonstrated under laboratory conditions. A virulent LSDV strain was injected into the thorax of flies to bypass the midgut barrier. The fate of the pathogen in the hemolymph of the flies was examined using PCR and virus isolation tests. LSDV was isolated from all three Stomoxys species up to 24h post inoculation while virus DNA was detectable up to 7d post inoculation.https://doi.org/10.1371/journal.pone.0238210 |
| spellingShingle | Arman Issimov David B Taylor Malik Shalmenov Birzhan Nurgaliyev Izimgali Zhubantayev Nurzhan Abekeshev Kaissar Kushaliyev Abzal Kereyev Lespek Kutumbetov Assylbek Zhanabayev Yasmin Zhakiyanova Peter J White Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae. PLoS ONE |
| title | Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae. |
| title_full | Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae. |
| title_fullStr | Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae. |
| title_full_unstemmed | Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae. |
| title_short | Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae. |
| title_sort | retention of lumpy skin disease virus in stomoxys spp stomoxys calcitrans stomoxys sitiens stomoxys indica following intrathoracic inoculation diptera muscidae |
| url | https://doi.org/10.1371/journal.pone.0238210 |
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