Quantitative Analysis of UV-B Radiation Interception in 3D Plant Structures and Intraindividual Distribution of Phenolic Contents
Ultraviolet-B (UV-B) acts as a regulatory stimulus, inducing the dose-dependent biosynthesis of phenolic compounds such as flavonoids at the leaf level. However, the heterogeneity of biosynthesis activation generated within a whole plant is not fully understood until now and cannot be interpreted wi...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/5/2701 |
| _version_ | 1797412553664495616 |
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| author | Hyo In Yoon Hyun Young Kim Jaewoo Kim Myung-Min Oh Jung Eek Son |
| author_facet | Hyo In Yoon Hyun Young Kim Jaewoo Kim Myung-Min Oh Jung Eek Son |
| author_sort | Hyo In Yoon |
| collection | DOAJ |
| description | Ultraviolet-B (UV-B) acts as a regulatory stimulus, inducing the dose-dependent biosynthesis of phenolic compounds such as flavonoids at the leaf level. However, the heterogeneity of biosynthesis activation generated within a whole plant is not fully understood until now and cannot be interpreted without quantification of UV-B radiation interception. In this study, we analyzed the spatial UV-B radiation interception of kales (<i>Brassica oleracea</i> L. var. <i>Acephala</i>) grown under supplemental UV-B LED using ray-tracing simulation with 3-dimension-scanned models and leaf optical properties. The UV-B-induced phenolic compounds and flavonoids accumulated more, with higher UV-B interception and younger leaves. To distinguish the effects of UV-B energy and leaf developmental age, the contents were regressed separately and simultaneously. The effect of intercepted UV-B on flavonoid content was 4.9-fold that of leaf age, but the effects on phenolic compound biosynthesis were similar. This study confirmed the feasibility and relevance of UV-B radiation interception analysis and paves the way to explore the physical and physiological base determining the intraindividual distribution of phenolic compound in controlled environments. |
| first_indexed | 2024-03-09T05:04:48Z |
| format | Article |
| id | doaj.art-2d766c24c3f847e5ae5b033628523a4e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:04:48Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2d766c24c3f847e5ae5b033628523a4e2023-12-03T12:55:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01225270110.3390/ijms22052701Quantitative Analysis of UV-B Radiation Interception in 3D Plant Structures and Intraindividual Distribution of Phenolic ContentsHyo In Yoon0Hyun Young Kim1Jaewoo Kim2Myung-Min Oh3Jung Eek Son4Department of Agriculture, Forestry and Bioresources (Horticultural Science and Biotechnology), Seoul National University, Seoul 08826, KoreaDepartment of Agriculture, Forestry and Bioresources (Horticultural Science and Biotechnology), Seoul National University, Seoul 08826, KoreaDepartment of Agriculture, Forestry and Bioresources (Horticultural Science and Biotechnology), Seoul National University, Seoul 08826, KoreaDivision of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 28644, KoreaDepartment of Agriculture, Forestry and Bioresources (Horticultural Science and Biotechnology), Seoul National University, Seoul 08826, KoreaUltraviolet-B (UV-B) acts as a regulatory stimulus, inducing the dose-dependent biosynthesis of phenolic compounds such as flavonoids at the leaf level. However, the heterogeneity of biosynthesis activation generated within a whole plant is not fully understood until now and cannot be interpreted without quantification of UV-B radiation interception. In this study, we analyzed the spatial UV-B radiation interception of kales (<i>Brassica oleracea</i> L. var. <i>Acephala</i>) grown under supplemental UV-B LED using ray-tracing simulation with 3-dimension-scanned models and leaf optical properties. The UV-B-induced phenolic compounds and flavonoids accumulated more, with higher UV-B interception and younger leaves. To distinguish the effects of UV-B energy and leaf developmental age, the contents were regressed separately and simultaneously. The effect of intercepted UV-B on flavonoid content was 4.9-fold that of leaf age, but the effects on phenolic compound biosynthesis were similar. This study confirmed the feasibility and relevance of UV-B radiation interception analysis and paves the way to explore the physical and physiological base determining the intraindividual distribution of phenolic compound in controlled environments.https://www.mdpi.com/1422-0067/22/5/2701antioxidant activity<i>Brassica oleracea</i>chlorophyll fluorescenceDPPHflavonoidskale |
| spellingShingle | Hyo In Yoon Hyun Young Kim Jaewoo Kim Myung-Min Oh Jung Eek Son Quantitative Analysis of UV-B Radiation Interception in 3D Plant Structures and Intraindividual Distribution of Phenolic Contents International Journal of Molecular Sciences antioxidant activity <i>Brassica oleracea</i> chlorophyll fluorescence DPPH flavonoids kale |
| title | Quantitative Analysis of UV-B Radiation Interception in 3D Plant Structures and Intraindividual Distribution of Phenolic Contents |
| title_full | Quantitative Analysis of UV-B Radiation Interception in 3D Plant Structures and Intraindividual Distribution of Phenolic Contents |
| title_fullStr | Quantitative Analysis of UV-B Radiation Interception in 3D Plant Structures and Intraindividual Distribution of Phenolic Contents |
| title_full_unstemmed | Quantitative Analysis of UV-B Radiation Interception in 3D Plant Structures and Intraindividual Distribution of Phenolic Contents |
| title_short | Quantitative Analysis of UV-B Radiation Interception in 3D Plant Structures and Intraindividual Distribution of Phenolic Contents |
| title_sort | quantitative analysis of uv b radiation interception in 3d plant structures and intraindividual distribution of phenolic contents |
| topic | antioxidant activity <i>Brassica oleracea</i> chlorophyll fluorescence DPPH flavonoids kale |
| url | https://www.mdpi.com/1422-0067/22/5/2701 |
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