Human Fibroblasts In Vitro Exposed to 2.45 GHz Continuous and Pulsed Wave Signals: Evaluation of Biological Effects with a Multimethodological Approach
The increasing exposure to radiofrequency electromagnetic fields (RF-EMF), especially from wireless communication devices, raises questions about their possible adverse health effects. So far, several in vitro studies evaluating RF-EMF genotoxic and cytotoxic non-thermal effects have reported contra...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/19/7069 |
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| author | Elisa Regalbuto Anna Anselmo Stefania De Sanctis Valeria Franchini Florigio Lista Monica Benvenuto Roberto Bei Laura Masuelli Guglielmo D’Inzeo Alessandra Paffi Eugenio Trodella Antonella Sgura |
| author_facet | Elisa Regalbuto Anna Anselmo Stefania De Sanctis Valeria Franchini Florigio Lista Monica Benvenuto Roberto Bei Laura Masuelli Guglielmo D’Inzeo Alessandra Paffi Eugenio Trodella Antonella Sgura |
| author_sort | Elisa Regalbuto |
| collection | DOAJ |
| description | The increasing exposure to radiofrequency electromagnetic fields (RF-EMF), especially from wireless communication devices, raises questions about their possible adverse health effects. So far, several in vitro studies evaluating RF-EMF genotoxic and cytotoxic non-thermal effects have reported contradictory results that could be mainly due to inadequate experimental design and lack of well-characterized exposure systems and conditions. Moreover, a topic poorly investigated is related to signal modulation induced by electromagnetic fields. The aim of this study was to perform an analysis of the potential non-thermal biological effects induced by 2.45 GHz exposures through a characterized exposure system and a multimethodological approach. Human fibroblasts were exposed to continuous (CW) and pulsed (PW) signals for 2 h in a wire patch cell-based exposure system at the specific absorption rate (SAR) of 0.7 W/kg. The evaluation of the potential biological effects was carried out through a multimethodological approach, including classical biological markers (genotoxic, cell cycle, and ultrastructural) and the evaluation of gene expression profile through the powerful high-throughput next generation sequencing (NGS) RNA sequencing (RNA-seq) approach. Our results suggest that 2.45 GHz radiofrequency fields did not induce significant biological effects at a cellular or molecular level for the evaluated exposure parameters and conditions. |
| first_indexed | 2024-03-10T16:02:29Z |
| format | Article |
| id | doaj.art-2d77121676144c0ab3cb42ca93fead42 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:02:29Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2d77121676144c0ab3cb42ca93fead422023-11-20T15:07:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-012119706910.3390/ijms21197069Human Fibroblasts In Vitro Exposed to 2.45 GHz Continuous and Pulsed Wave Signals: Evaluation of Biological Effects with a Multimethodological ApproachElisa Regalbuto0Anna Anselmo1Stefania De Sanctis2Valeria Franchini3Florigio Lista4Monica Benvenuto5Roberto Bei6Laura Masuelli7Guglielmo D’Inzeo8Alessandra Paffi9Eugenio Trodella10Antonella Sgura11Scientific Department, Army Medical Center of Rome, 00184 Rome, ItalyScientific Department, Army Medical Center of Rome, 00184 Rome, ItalyScientific Department, Army Medical Center of Rome, 00184 Rome, ItalyScientific Department, Army Medical Center of Rome, 00184 Rome, ItalyScientific Department, Army Medical Center of Rome, 00184 Rome, ItalySaint Camillus International University of Health and Medical Sciences, 00131 Rome, ItalyDepartment of Clinical Sciences and Translational Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Experimental Medicine, University of Rome “Sapienza”, 00161 Rome, ItalyDepartment of Information Engineering, Electronics and Telecommunications (DIET), University of Rome “La Sapienza”, 00184 Rome, ItalyDepartment of Information Engineering, Electronics and Telecommunications (DIET), University of Rome “La Sapienza”, 00184 Rome, ItalyDepartment of Information Engineering, Electronics and Telecommunications (DIET), University of Rome “La Sapienza”, 00184 Rome, ItalyDepartment of Science, University of Rome “Roma Tre”, 00146 Rome, ItalyThe increasing exposure to radiofrequency electromagnetic fields (RF-EMF), especially from wireless communication devices, raises questions about their possible adverse health effects. So far, several in vitro studies evaluating RF-EMF genotoxic and cytotoxic non-thermal effects have reported contradictory results that could be mainly due to inadequate experimental design and lack of well-characterized exposure systems and conditions. Moreover, a topic poorly investigated is related to signal modulation induced by electromagnetic fields. The aim of this study was to perform an analysis of the potential non-thermal biological effects induced by 2.45 GHz exposures through a characterized exposure system and a multimethodological approach. Human fibroblasts were exposed to continuous (CW) and pulsed (PW) signals for 2 h in a wire patch cell-based exposure system at the specific absorption rate (SAR) of 0.7 W/kg. The evaluation of the potential biological effects was carried out through a multimethodological approach, including classical biological markers (genotoxic, cell cycle, and ultrastructural) and the evaluation of gene expression profile through the powerful high-throughput next generation sequencing (NGS) RNA sequencing (RNA-seq) approach. Our results suggest that 2.45 GHz radiofrequency fields did not induce significant biological effects at a cellular or molecular level for the evaluated exposure parameters and conditions.https://www.mdpi.com/1422-0067/21/19/70692.45 GHzWi-Figenotoxic effectgene expressionRNA sequencing (RNA-seq)RT-PCR |
| spellingShingle | Elisa Regalbuto Anna Anselmo Stefania De Sanctis Valeria Franchini Florigio Lista Monica Benvenuto Roberto Bei Laura Masuelli Guglielmo D’Inzeo Alessandra Paffi Eugenio Trodella Antonella Sgura Human Fibroblasts In Vitro Exposed to 2.45 GHz Continuous and Pulsed Wave Signals: Evaluation of Biological Effects with a Multimethodological Approach International Journal of Molecular Sciences 2.45 GHz Wi-Fi genotoxic effect gene expression RNA sequencing (RNA-seq) RT-PCR |
| title | Human Fibroblasts In Vitro Exposed to 2.45 GHz Continuous and Pulsed Wave Signals: Evaluation of Biological Effects with a Multimethodological Approach |
| title_full | Human Fibroblasts In Vitro Exposed to 2.45 GHz Continuous and Pulsed Wave Signals: Evaluation of Biological Effects with a Multimethodological Approach |
| title_fullStr | Human Fibroblasts In Vitro Exposed to 2.45 GHz Continuous and Pulsed Wave Signals: Evaluation of Biological Effects with a Multimethodological Approach |
| title_full_unstemmed | Human Fibroblasts In Vitro Exposed to 2.45 GHz Continuous and Pulsed Wave Signals: Evaluation of Biological Effects with a Multimethodological Approach |
| title_short | Human Fibroblasts In Vitro Exposed to 2.45 GHz Continuous and Pulsed Wave Signals: Evaluation of Biological Effects with a Multimethodological Approach |
| title_sort | human fibroblasts in vitro exposed to 2 45 ghz continuous and pulsed wave signals evaluation of biological effects with a multimethodological approach |
| topic | 2.45 GHz Wi-Fi genotoxic effect gene expression RNA sequencing (RNA-seq) RT-PCR |
| url | https://www.mdpi.com/1422-0067/21/19/7069 |
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