Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison
Abstract Background Melioidosis is a serious bacterial infection caused by Burkholderia pseudomallei, a gram-negative bacterium commonly found in soil and water. It can affect both humans and animals, and is endemic in regions such as Southeast Asia and Northern Australia. In recent years, there hav...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-024-03957-5 |
| _version_ | 1797247185614536704 |
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| author | Anais Desoutter Thomas Deshayes Fabien Vorimore Bernice Klotoe Benoit Durand Julien Colot Gabriel Wagner-Lichtenegger Ivo Steinmetz Apichai Tuanyok Karine Laroucau |
| author_facet | Anais Desoutter Thomas Deshayes Fabien Vorimore Bernice Klotoe Benoit Durand Julien Colot Gabriel Wagner-Lichtenegger Ivo Steinmetz Apichai Tuanyok Karine Laroucau |
| author_sort | Anais Desoutter |
| collection | DOAJ |
| description | Abstract Background Melioidosis is a serious bacterial infection caused by Burkholderia pseudomallei, a gram-negative bacterium commonly found in soil and water. It can affect both humans and animals, and is endemic in regions such as Southeast Asia and Northern Australia. In recent years, there have been reports of an emergence of human melioidosis in other areas, including New Caledonia. Results During standard laboratory analysis in New Caledonia in 2021, a strain of B. pseudomallei was isolated from a goat. The strain was characterized using both MLST and WGS techniques and was found to cluster with previously described local human strains from the area. In parallel, several serological tests (CFT, ELISA, Luminex (Hcp1, GroEL, BPSS1840), arrays assay and a latex agglutination test) were performed on animals from the farm where the goat originated, and/or from three other neighboring farms. Using two commercial ELISA kits, seropositive animals were found only on the farm where the infected goat originated and tests based on recombinant proteins confirmed the usefulness of the Hcp1 protein for the diagnosis of melioidosis in animals. Conclusions Despite the regular reports of human cases, this is the first confirmed case of melioidosis in an animal in New Caledonia. These results confirm the presence of the bacterium in the region and highlight the importance of vigilance for both animal and human health. It is critical that all health partners, including breeders, veterinarians, and biologists, work together to monitor and prevent the spread of the disease. |
| first_indexed | 2024-04-24T19:54:41Z |
| format | Article |
| id | doaj.art-2d7f2044f15c403185baba4c211e0dd2 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-24T19:54:41Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-2d7f2044f15c403185baba4c211e0dd22024-03-24T12:23:47ZengBMCBMC Veterinary Research1746-61482024-03-0120111210.1186/s12917-024-03957-5Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparisonAnais Desoutter0Thomas Deshayes1Fabien Vorimore2Bernice Klotoe3Benoit Durand4Julien Colot5Gabriel Wagner-Lichtenegger6Ivo Steinmetz7Apichai Tuanyok8Karine Laroucau9LNC (Laboratory of New Caledonia), Animal Health DepartmentAnses, Animal Health Laboratory, Bacterial Zoonosis UnitAnses, Animal Health Laboratory, Bacterial Zoonosis UnitAnses, Animal Health Laboratory, Bacterial Zoonosis UnitAnses, Animal Health Laboratory, Epidemiological UnitTerritorial Hospital Center Gaston-Bourret, Medical Biology LaboratoryDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of GrazDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of GrazDepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, and Emerging Pathogens Institute, University of FloridaAnses, Animal Health Laboratory, Bacterial Zoonosis UnitAbstract Background Melioidosis is a serious bacterial infection caused by Burkholderia pseudomallei, a gram-negative bacterium commonly found in soil and water. It can affect both humans and animals, and is endemic in regions such as Southeast Asia and Northern Australia. In recent years, there have been reports of an emergence of human melioidosis in other areas, including New Caledonia. Results During standard laboratory analysis in New Caledonia in 2021, a strain of B. pseudomallei was isolated from a goat. The strain was characterized using both MLST and WGS techniques and was found to cluster with previously described local human strains from the area. In parallel, several serological tests (CFT, ELISA, Luminex (Hcp1, GroEL, BPSS1840), arrays assay and a latex agglutination test) were performed on animals from the farm where the goat originated, and/or from three other neighboring farms. Using two commercial ELISA kits, seropositive animals were found only on the farm where the infected goat originated and tests based on recombinant proteins confirmed the usefulness of the Hcp1 protein for the diagnosis of melioidosis in animals. Conclusions Despite the regular reports of human cases, this is the first confirmed case of melioidosis in an animal in New Caledonia. These results confirm the presence of the bacterium in the region and highlight the importance of vigilance for both animal and human health. It is critical that all health partners, including breeders, veterinarians, and biologists, work together to monitor and prevent the spread of the disease.https://doi.org/10.1186/s12917-024-03957-5Burkholderia pseudomalleiNew CaledoniaGoatSerologyELISALuminex |
| spellingShingle | Anais Desoutter Thomas Deshayes Fabien Vorimore Bernice Klotoe Benoit Durand Julien Colot Gabriel Wagner-Lichtenegger Ivo Steinmetz Apichai Tuanyok Karine Laroucau Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison BMC Veterinary Research Burkholderia pseudomallei New Caledonia Goat Serology ELISA Luminex |
| title | Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison |
| title_full | Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison |
| title_fullStr | Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison |
| title_full_unstemmed | Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison |
| title_short | Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison |
| title_sort | isolation of burkholderia pseudomallei from a goat in new caledonia implications for animal and human health monitoring and serological tool comparison |
| topic | Burkholderia pseudomallei New Caledonia Goat Serology ELISA Luminex |
| url | https://doi.org/10.1186/s12917-024-03957-5 |
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