Population Kinetics and Mechanistic Aspects of Saccharomyces cerevisiae Growth in Relation to Selenium Sulfide Nanoparticle Synthesis
Biosynthesis of nanoparticles (NPs) by microorganisms is a cost- and energy-effective approach. However, how the production of NPs affects the population of producing organism remains as an unresolved question. The present study aimed to evaluate the kinetics of Saccharomyces cerevisiae growth in re...
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| Format: | Article |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01019/full |
| _version_ | 1818550658060517376 |
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| author | Farnoush Asghari-Paskiabi Farnoush Asghari-Paskiabi Mohammad Imani Sana Eybpoosh Hashem Rafii-Tabar Mehdi Razzaghi-Abyaneh |
| author_facet | Farnoush Asghari-Paskiabi Farnoush Asghari-Paskiabi Mohammad Imani Sana Eybpoosh Hashem Rafii-Tabar Mehdi Razzaghi-Abyaneh |
| author_sort | Farnoush Asghari-Paskiabi |
| collection | DOAJ |
| description | Biosynthesis of nanoparticles (NPs) by microorganisms is a cost- and energy-effective approach. However, how the production of NPs affects the population of producing organism remains as an unresolved question. The present study aimed to evaluate the kinetics of Saccharomyces cerevisiae growth in relation to synthesis of selenium sulfide nanoparticles by using a population model. To this end, the population of S. cerevisiae cells was investigated in terms of colony forming units (CFU) in the presence of the substrate in different time points. Fluctuation of sulfite reductase (SiR) activity, expression of MET5 and MET10 genes, and concentrations of sulfite and selenium were evaluated to support the population findings. CFU values in the test groups were lower than those in the control counterparts. The rise and fall of the SiR activity and MET5 and MET10 gene expression conformed to the variations of CFU values. The rate of reduction in the selenium and sulfite concentrations tended to decrease over the time. In conclusion, the cells population was negatively and positively affected by selenium and sulfite concentrations, respectively. The indirect relationship of the selenium ions concentration in the path analysis revealed that the product, selenium sulfide nanoparticles, caused this drop in S. cerevisiae cells population. |
| first_indexed | 2024-12-12T08:49:25Z |
| format | Article |
| id | doaj.art-2d7fadc886ad4b7f90ad133679c43e1f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T08:49:25Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2d7fadc886ad4b7f90ad133679c43e1f2022-12-22T00:30:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-05-011110.3389/fmicb.2020.01019537293Population Kinetics and Mechanistic Aspects of Saccharomyces cerevisiae Growth in Relation to Selenium Sulfide Nanoparticle SynthesisFarnoush Asghari-Paskiabi0Farnoush Asghari-Paskiabi1Mohammad Imani2Sana Eybpoosh3Hashem Rafii-Tabar4Mehdi Razzaghi-Abyaneh5Department of Medical Physics and Biomedical Engineering, School of Medicine, Shahid Beheshti University of Medical Science, Tehran, IranDepartment of Mycology, Pasteur Institute of Iran, Tehran, IranNovel Drug Delivery Systems Department, Iran Polymer and Petrochemical Institute, Tehran, IranDepartment of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, IranDepartment of Medical Physics and Biomedical Engineering, School of Medicine, Shahid Beheshti University of Medical Science, Tehran, IranDepartment of Mycology, Pasteur Institute of Iran, Tehran, IranBiosynthesis of nanoparticles (NPs) by microorganisms is a cost- and energy-effective approach. However, how the production of NPs affects the population of producing organism remains as an unresolved question. The present study aimed to evaluate the kinetics of Saccharomyces cerevisiae growth in relation to synthesis of selenium sulfide nanoparticles by using a population model. To this end, the population of S. cerevisiae cells was investigated in terms of colony forming units (CFU) in the presence of the substrate in different time points. Fluctuation of sulfite reductase (SiR) activity, expression of MET5 and MET10 genes, and concentrations of sulfite and selenium were evaluated to support the population findings. CFU values in the test groups were lower than those in the control counterparts. The rise and fall of the SiR activity and MET5 and MET10 gene expression conformed to the variations of CFU values. The rate of reduction in the selenium and sulfite concentrations tended to decrease over the time. In conclusion, the cells population was negatively and positively affected by selenium and sulfite concentrations, respectively. The indirect relationship of the selenium ions concentration in the path analysis revealed that the product, selenium sulfide nanoparticles, caused this drop in S. cerevisiae cells population.https://www.frontiersin.org/article/10.3389/fmicb.2020.01019/fullSaccharomyces cerevisiaeselenium sulfide nanoparticlessulfite reductasemodelingGrowth Kinetics |
| spellingShingle | Farnoush Asghari-Paskiabi Farnoush Asghari-Paskiabi Mohammad Imani Sana Eybpoosh Hashem Rafii-Tabar Mehdi Razzaghi-Abyaneh Population Kinetics and Mechanistic Aspects of Saccharomyces cerevisiae Growth in Relation to Selenium Sulfide Nanoparticle Synthesis Frontiers in Microbiology Saccharomyces cerevisiae selenium sulfide nanoparticles sulfite reductase modeling Growth Kinetics |
| title | Population Kinetics and Mechanistic Aspects of Saccharomyces cerevisiae Growth in Relation to Selenium Sulfide Nanoparticle Synthesis |
| title_full | Population Kinetics and Mechanistic Aspects of Saccharomyces cerevisiae Growth in Relation to Selenium Sulfide Nanoparticle Synthesis |
| title_fullStr | Population Kinetics and Mechanistic Aspects of Saccharomyces cerevisiae Growth in Relation to Selenium Sulfide Nanoparticle Synthesis |
| title_full_unstemmed | Population Kinetics and Mechanistic Aspects of Saccharomyces cerevisiae Growth in Relation to Selenium Sulfide Nanoparticle Synthesis |
| title_short | Population Kinetics and Mechanistic Aspects of Saccharomyces cerevisiae Growth in Relation to Selenium Sulfide Nanoparticle Synthesis |
| title_sort | population kinetics and mechanistic aspects of saccharomyces cerevisiae growth in relation to selenium sulfide nanoparticle synthesis |
| topic | Saccharomyces cerevisiae selenium sulfide nanoparticles sulfite reductase modeling Growth Kinetics |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01019/full |
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