Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previo...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
1991-06-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821991000200003 |
| _version_ | 1818200255422791680 |
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| author | Claudia Lamarca Vitral Ana Maria Coimbra Gaspar Clara Fumiko Tachibana Yoshida |
| author_facet | Claudia Lamarca Vitral Ana Maria Coimbra Gaspar Clara Fumiko Tachibana Yoshida |
| author_sort | Claudia Lamarca Vitral |
| collection | DOAJ |
| description | Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.<br>Foram desenvolvidos dois ensaios imunoenzimáticos (EIA) competitivos: no primeiro (COMP-1) colocou-se numa placa sensibilizada com anti-HAVIgG as amostras teste juntamente como antígeno HA Vea seguir o conjugado anti-HA VHRP; no segundo (COMP-2), as amostras teste e o conjugado anti-HAV HRP competem pelos epitopos do antígeno HAV previamente absorvido na placa sensibilizada do anti-HAV IgG. O substrato utilizado foi tetrametilbenzidina (TMB). Ambas as técnicas mostraram ser produtíveis e aplicáveis para fins de diagnóstico e pesquisa. |
| first_indexed | 2024-12-12T02:34:45Z |
| format | Article |
| id | doaj.art-2d807cb387f748128c533b8daf72cce5 |
| institution | Directory Open Access Journal |
| issn | 0037-8682 1678-9849 |
| language | English |
| last_indexed | 2024-12-12T02:34:45Z |
| publishDate | 1991-06-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-2d807cb387f748128c533b8daf72cce52022-12-22T00:41:19ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical0037-86821678-98491991-06-012427985Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigenClaudia Lamarca VitralAna Maria Coimbra GasparClara Fumiko Tachibana YoshidaTwo competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.<br>Foram desenvolvidos dois ensaios imunoenzimáticos (EIA) competitivos: no primeiro (COMP-1) colocou-se numa placa sensibilizada com anti-HAVIgG as amostras teste juntamente como antígeno HA Vea seguir o conjugado anti-HA VHRP; no segundo (COMP-2), as amostras teste e o conjugado anti-HAV HRP competem pelos epitopos do antígeno HAV previamente absorvido na placa sensibilizada do anti-HAV IgG. O substrato utilizado foi tetrametilbenzidina (TMB). Ambas as técnicas mostraram ser produtíveis e aplicáveis para fins de diagnóstico e pesquisa.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821991000200003Enzyme immmunoassayAnti-HAVHepatitis AEnzyme immunoassayAnti-HAVHepatitis A |
| spellingShingle | Claudia Lamarca Vitral Ana Maria Coimbra Gaspar Clara Fumiko Tachibana Yoshida Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen Revista da Sociedade Brasileira de Medicina Tropical Enzyme immmunoassay Anti-HAV Hepatitis A Enzyme immunoassay Anti-HAV Hepatitis A |
| title | Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
| title_full | Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
| title_fullStr | Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
| title_full_unstemmed | Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
| title_short | Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen |
| title_sort | two competitive enzyme immunoassays for the detection of igg class antibodies to hepatitis a antigen |
| topic | Enzyme immmunoassay Anti-HAV Hepatitis A Enzyme immunoassay Anti-HAV Hepatitis A |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821991000200003 |
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