The DNA barcoding project on German Diptera: An appreciative and critical analysis with four suggestions for improving the development and reliability of DNA-based identification
The progress in constructing a DNA barcode library for German Diptera as published by Morinière et al. (2019, Mol. Ecol. Resour. 19: 900-928) is appraised from a dipterists' perspective. The coverage of the diversity of German Diptera in terms of barcode index numbers (BINs) and identified barc...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2020-07-01
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| Series: | European Journal of Entomology |
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| Online Access: | https://www.eje.cz/artkey/eje-202001-0037_the_dna_barcoding_project_on_german_diptera_an_appreciative_and_critical_analysis_with_four_suggestions_for_im.php |
| _version_ | 1818655619776774144 |
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| author | Marion KOTRBA |
| author_facet | Marion KOTRBA |
| author_sort | Marion KOTRBA |
| collection | DOAJ |
| description | The progress in constructing a DNA barcode library for German Diptera as published by Morinière et al. (2019, Mol. Ecol. Resour. 19: 900-928) is appraised from a dipterists' perspective. The coverage of the diversity of German Diptera in terms of barcode index numbers (BINs) and identified barcodes is analysed and visualized in simple diagrams. The influence of the project setup, methodology and/or systematic effects on the emerging numbers and trends is elucidated and extensively discussed. In addition, the documentation on the species identification methods in the database is assessed. Based on this evaluation, four ways for improving the future development, utility and reliability of this DNA database and similar projects in general are identified: (1) Sample the collections of experts. This results in a greater and more reliable coverage within a limited time frame, as opposed to random collecting and relying on a posteriori identification. (2) Give priority to medically, agriculturally or ecologically important families. Addressing these gaps will meet the most pressing needs of the community and serve as a good advertisement for the usefulness and wide applicability of the DNA barcode library. (3) Allocate resources to recruiting established experts as opposed to trainees. The fact that half of the recovered BINs remained unidentified mostly results from the insufficient involvement of experts (and expert time). (4) Appropriately document the morphological identifications by experts in the database. This will allow to assess the reliability of DNA-based identifications and to prioritize conflicting identifications within a BIN accordingly. |
| first_indexed | 2024-12-17T03:12:34Z |
| format | Article |
| id | doaj.art-2d80c63b6f0f409aad64991ba4feedc3 |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-17T03:12:34Z |
| publishDate | 2020-07-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-2d80c63b6f0f409aad64991ba4feedc32022-12-21T22:05:47ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292020-07-01117131532710.14411/eje.2020.037eje-202001-0037The DNA barcoding project on German Diptera: An appreciative and critical analysis with four suggestions for improving the development and reliability of DNA-based identificationMarion KOTRBA0SNSB-Zoologische Staatssammlung München, Münchhausenstraße 21, D-81247 München, Germany; e-mail: kotrba@snsb.deThe progress in constructing a DNA barcode library for German Diptera as published by Morinière et al. (2019, Mol. Ecol. Resour. 19: 900-928) is appraised from a dipterists' perspective. The coverage of the diversity of German Diptera in terms of barcode index numbers (BINs) and identified barcodes is analysed and visualized in simple diagrams. The influence of the project setup, methodology and/or systematic effects on the emerging numbers and trends is elucidated and extensively discussed. In addition, the documentation on the species identification methods in the database is assessed. Based on this evaluation, four ways for improving the future development, utility and reliability of this DNA database and similar projects in general are identified: (1) Sample the collections of experts. This results in a greater and more reliable coverage within a limited time frame, as opposed to random collecting and relying on a posteriori identification. (2) Give priority to medically, agriculturally or ecologically important families. Addressing these gaps will meet the most pressing needs of the community and serve as a good advertisement for the usefulness and wide applicability of the DNA barcode library. (3) Allocate resources to recruiting established experts as opposed to trainees. The fact that half of the recovered BINs remained unidentified mostly results from the insufficient involvement of experts (and expert time). (4) Appropriately document the morphological identifications by experts in the database. This will allow to assess the reliability of DNA-based identifications and to prioritize conflicting identifications within a BIN accordingly.https://www.eje.cz/artkey/eje-202001-0037_the_dna_barcoding_project_on_german_diptera_an_appreciative_and_critical_analysis_with_four_suggestions_for_im.phpdipteradna barcodinggbolgermanyboldbiodiversityidentification |
| spellingShingle | Marion KOTRBA The DNA barcoding project on German Diptera: An appreciative and critical analysis with four suggestions for improving the development and reliability of DNA-based identification European Journal of Entomology diptera dna barcoding gbol germany bold biodiversity identification |
| title | The DNA barcoding project on German Diptera: An appreciative and critical analysis with four suggestions for improving the development and reliability of DNA-based identification |
| title_full | The DNA barcoding project on German Diptera: An appreciative and critical analysis with four suggestions for improving the development and reliability of DNA-based identification |
| title_fullStr | The DNA barcoding project on German Diptera: An appreciative and critical analysis with four suggestions for improving the development and reliability of DNA-based identification |
| title_full_unstemmed | The DNA barcoding project on German Diptera: An appreciative and critical analysis with four suggestions for improving the development and reliability of DNA-based identification |
| title_short | The DNA barcoding project on German Diptera: An appreciative and critical analysis with four suggestions for improving the development and reliability of DNA-based identification |
| title_sort | dna barcoding project on german diptera an appreciative and critical analysis with four suggestions for improving the development and reliability of dna based identification |
| topic | diptera dna barcoding gbol germany bold biodiversity identification |
| url | https://www.eje.cz/artkey/eje-202001-0037_the_dna_barcoding_project_on_german_diptera_an_appreciative_and_critical_analysis_with_four_suggestions_for_im.php |
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