Characterization of ecto- and endoparasite communities of wild Mediterranean teleosts by a metabarcoding approach.
Next-generation sequencing methods are increasingly used to identify eukaryotic, unicellular and multicellular symbiont communities within hosts. In this study, we analyzed the non-specific reads obtained during a metabarcoding survey of the bacterial communities associated to three different tissue...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0221475 |
| _version_ | 1818586655339053056 |
|---|---|
| author | Mathilde Scheifler Magdalena Ruiz-Rodríguez Sophie Sanchez-Brosseau Elodie Magnanou Marcelino T Suzuki Nyree West Sébastien Duperron Yves Desdevises |
| author_facet | Mathilde Scheifler Magdalena Ruiz-Rodríguez Sophie Sanchez-Brosseau Elodie Magnanou Marcelino T Suzuki Nyree West Sébastien Duperron Yves Desdevises |
| author_sort | Mathilde Scheifler |
| collection | DOAJ |
| description | Next-generation sequencing methods are increasingly used to identify eukaryotic, unicellular and multicellular symbiont communities within hosts. In this study, we analyzed the non-specific reads obtained during a metabarcoding survey of the bacterial communities associated to three different tissues collected from 13 wild Mediterranean teleost fish species. In total, 30 eukaryotic genera were identified as putative parasites of teleosts, associated to skin mucus, gills mucus and intestine: 2 ascomycetes, 4 arthropods, 2 cnidarians, 7 nematodes, 10 platyhelminthes, 4 apicomplexans, 1 ciliate as well as one order in dinoflagellates (Syndiniales). These results highlighted that (1) the metabarcoding approach was able to uncover a large spectrum of symbiotic organisms associated to the fish species studied, (2) symbionts not yet identified in several teleost species were putatively present, (3) the parasitic diversity differed markedly across host species and (4) in most cases, the distribution of known parasitic genera within tissues is in accordance with the literature. The current work illustrates the large insights that can be gained by making maximum use of data from a metabarcoding approach. |
| first_indexed | 2024-12-16T08:56:25Z |
| format | Article |
| id | doaj.art-2d8214417d974149a1ddfcf53567cc09 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T08:56:25Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2d8214417d974149a1ddfcf53567cc092022-12-21T22:37:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01149e022147510.1371/journal.pone.0221475Characterization of ecto- and endoparasite communities of wild Mediterranean teleosts by a metabarcoding approach.Mathilde ScheiflerMagdalena Ruiz-RodríguezSophie Sanchez-BrosseauElodie MagnanouMarcelino T SuzukiNyree WestSébastien DuperronYves DesdevisesNext-generation sequencing methods are increasingly used to identify eukaryotic, unicellular and multicellular symbiont communities within hosts. In this study, we analyzed the non-specific reads obtained during a metabarcoding survey of the bacterial communities associated to three different tissues collected from 13 wild Mediterranean teleost fish species. In total, 30 eukaryotic genera were identified as putative parasites of teleosts, associated to skin mucus, gills mucus and intestine: 2 ascomycetes, 4 arthropods, 2 cnidarians, 7 nematodes, 10 platyhelminthes, 4 apicomplexans, 1 ciliate as well as one order in dinoflagellates (Syndiniales). These results highlighted that (1) the metabarcoding approach was able to uncover a large spectrum of symbiotic organisms associated to the fish species studied, (2) symbionts not yet identified in several teleost species were putatively present, (3) the parasitic diversity differed markedly across host species and (4) in most cases, the distribution of known parasitic genera within tissues is in accordance with the literature. The current work illustrates the large insights that can be gained by making maximum use of data from a metabarcoding approach.https://doi.org/10.1371/journal.pone.0221475 |
| spellingShingle | Mathilde Scheifler Magdalena Ruiz-Rodríguez Sophie Sanchez-Brosseau Elodie Magnanou Marcelino T Suzuki Nyree West Sébastien Duperron Yves Desdevises Characterization of ecto- and endoparasite communities of wild Mediterranean teleosts by a metabarcoding approach. PLoS ONE |
| title | Characterization of ecto- and endoparasite communities of wild Mediterranean teleosts by a metabarcoding approach. |
| title_full | Characterization of ecto- and endoparasite communities of wild Mediterranean teleosts by a metabarcoding approach. |
| title_fullStr | Characterization of ecto- and endoparasite communities of wild Mediterranean teleosts by a metabarcoding approach. |
| title_full_unstemmed | Characterization of ecto- and endoparasite communities of wild Mediterranean teleosts by a metabarcoding approach. |
| title_short | Characterization of ecto- and endoparasite communities of wild Mediterranean teleosts by a metabarcoding approach. |
| title_sort | characterization of ecto and endoparasite communities of wild mediterranean teleosts by a metabarcoding approach |
| url | https://doi.org/10.1371/journal.pone.0221475 |
| work_keys_str_mv | AT mathildescheifler characterizationofectoandendoparasitecommunitiesofwildmediterraneanteleostsbyametabarcodingapproach AT magdalenaruizrodriguez characterizationofectoandendoparasitecommunitiesofwildmediterraneanteleostsbyametabarcodingapproach AT sophiesanchezbrosseau characterizationofectoandendoparasitecommunitiesofwildmediterraneanteleostsbyametabarcodingapproach AT elodiemagnanou characterizationofectoandendoparasitecommunitiesofwildmediterraneanteleostsbyametabarcodingapproach AT marcelinotsuzuki characterizationofectoandendoparasitecommunitiesofwildmediterraneanteleostsbyametabarcodingapproach AT nyreewest characterizationofectoandendoparasitecommunitiesofwildmediterraneanteleostsbyametabarcodingapproach AT sebastienduperron characterizationofectoandendoparasitecommunitiesofwildmediterraneanteleostsbyametabarcodingapproach AT yvesdesdevises characterizationofectoandendoparasitecommunitiesofwildmediterraneanteleostsbyametabarcodingapproach |