Study on the Common Molecular Mechanism of Metabolic Acidosis and Myocardial Damage Complicated by Neonatal Pneumonia
Pneumonia is a common clinical disease in the neonatal period and poses a serious risk to infant health. Therefore, the understanding of molecular mechanisms is of great importance for the development of methods for the rapid and accurate identification, classification and staging, and even disease...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/11/1118 |
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| author | Yifei Zhan Huaiyan Wang Zeying Wu Zhongda Zeng |
| author_facet | Yifei Zhan Huaiyan Wang Zeying Wu Zhongda Zeng |
| author_sort | Yifei Zhan |
| collection | DOAJ |
| description | Pneumonia is a common clinical disease in the neonatal period and poses a serious risk to infant health. Therefore, the understanding of molecular mechanisms is of great importance for the development of methods for the rapid and accurate identification, classification and staging, and even disease diagnosis and therapy of pneumonia. In this study, a nontargeted metabonomic method was developed and applied for the analysis of serum samples collected from 20 cases in the pneumonia control group (PN) and 20 and 10 cases of pneumonia patients with metabolic acidosis (MA) and myocardial damage (MD), respectively, with the help of ultrahigh-performance liquid chromatography–high-resolution mass spectrometry (UPLC–HRMS). The results showed that compared with the pneumonia group, 23 and 21 differential metabolites were identified in pneumonia with two complications. They showed high sensitivity and specificity, with the area under the curve (ROC) of the receiver operating characteristic curve (ROC) larger than 0.7 for each differential molecule. There were 14 metabolites and three metabolic pathways of sphingolipid metabolism, porphyrin and chlorophyll metabolism, and glycerophospholipid metabolism existing in both groups of PN and MA, and PN and MD, all involving significant changes in pathways closely related to amino acid metabolism disorders, abnormal cell apoptosis, and inflammatory responses. These findings of molecular mechanisms should help a lot to fully understand and even treat the complications of pneumonia in infants. |
| first_indexed | 2024-03-09T16:36:58Z |
| format | Article |
| id | doaj.art-2d8708c4e6ed44dbb72ff56d20f6c92e |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T16:36:58Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-2d8708c4e6ed44dbb72ff56d20f6c92e2023-11-24T14:55:22ZengMDPI AGMetabolites2218-19892023-10-011311111810.3390/metabo13111118Study on the Common Molecular Mechanism of Metabolic Acidosis and Myocardial Damage Complicated by Neonatal PneumoniaYifei Zhan0Huaiyan Wang1Zeying Wu2Zhongda Zeng3College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, ChinaDepartment of Neonatology, Changzhou Medical Center, Changzhou Maternity and Child Health Care Hospital, Nanjing Medical University, Changzhou 213000, ChinaState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210023, ChinaCollege of Environmental and Chemical Engineering, Dalian University, Dalian 116622, ChinaPneumonia is a common clinical disease in the neonatal period and poses a serious risk to infant health. Therefore, the understanding of molecular mechanisms is of great importance for the development of methods for the rapid and accurate identification, classification and staging, and even disease diagnosis and therapy of pneumonia. In this study, a nontargeted metabonomic method was developed and applied for the analysis of serum samples collected from 20 cases in the pneumonia control group (PN) and 20 and 10 cases of pneumonia patients with metabolic acidosis (MA) and myocardial damage (MD), respectively, with the help of ultrahigh-performance liquid chromatography–high-resolution mass spectrometry (UPLC–HRMS). The results showed that compared with the pneumonia group, 23 and 21 differential metabolites were identified in pneumonia with two complications. They showed high sensitivity and specificity, with the area under the curve (ROC) of the receiver operating characteristic curve (ROC) larger than 0.7 for each differential molecule. There were 14 metabolites and three metabolic pathways of sphingolipid metabolism, porphyrin and chlorophyll metabolism, and glycerophospholipid metabolism existing in both groups of PN and MA, and PN and MD, all involving significant changes in pathways closely related to amino acid metabolism disorders, abnormal cell apoptosis, and inflammatory responses. These findings of molecular mechanisms should help a lot to fully understand and even treat the complications of pneumonia in infants.https://www.mdpi.com/2218-1989/13/11/1118UPLC–HRMS-based metabolomicschemometricspneumoniametabolic acidosismyocardial damage |
| spellingShingle | Yifei Zhan Huaiyan Wang Zeying Wu Zhongda Zeng Study on the Common Molecular Mechanism of Metabolic Acidosis and Myocardial Damage Complicated by Neonatal Pneumonia Metabolites UPLC–HRMS-based metabolomics chemometrics pneumonia metabolic acidosis myocardial damage |
| title | Study on the Common Molecular Mechanism of Metabolic Acidosis and Myocardial Damage Complicated by Neonatal Pneumonia |
| title_full | Study on the Common Molecular Mechanism of Metabolic Acidosis and Myocardial Damage Complicated by Neonatal Pneumonia |
| title_fullStr | Study on the Common Molecular Mechanism of Metabolic Acidosis and Myocardial Damage Complicated by Neonatal Pneumonia |
| title_full_unstemmed | Study on the Common Molecular Mechanism of Metabolic Acidosis and Myocardial Damage Complicated by Neonatal Pneumonia |
| title_short | Study on the Common Molecular Mechanism of Metabolic Acidosis and Myocardial Damage Complicated by Neonatal Pneumonia |
| title_sort | study on the common molecular mechanism of metabolic acidosis and myocardial damage complicated by neonatal pneumonia |
| topic | UPLC–HRMS-based metabolomics chemometrics pneumonia metabolic acidosis myocardial damage |
| url | https://www.mdpi.com/2218-1989/13/11/1118 |
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