Genetic and hosts characterization of hantaviruses in port areas in Hainan Province, P. R. China.
<h4>Background</h4>Hantaviruses (HVs) are major zoonotic pathogens in China that cause hemorrhagic fever with renal syndrome (HFRS) posing a major threat to people's health. Hainan Province, an island located in Southeast China, is an ideal region for sea ports. The unique tropical...
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0264859&type=printable |
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| author | Qiu-Wei Wang Li Tao Su-Ying Lu Chang-Qiang Zhu Le-le Ai Yizhe Luo Rong-Bin Yu Heng Lv Yun Zhang Chong-Cai Wang Wei-Long Tan |
| author_facet | Qiu-Wei Wang Li Tao Su-Ying Lu Chang-Qiang Zhu Le-le Ai Yizhe Luo Rong-Bin Yu Heng Lv Yun Zhang Chong-Cai Wang Wei-Long Tan |
| author_sort | Qiu-Wei Wang |
| collection | DOAJ |
| description | <h4>Background</h4>Hantaviruses (HVs) are major zoonotic pathogens in China that cause hemorrhagic fever with renal syndrome (HFRS) posing a major threat to people's health. Hainan Province, an island located in Southeast China, is an ideal region for sea ports. The unique tropical monsoon climate in Hainan provides sufficient living conditions for rodents, which help spread HVs and other rodent-borne diseases. In the routine monitoring of hantavirus, there was no evidence that rodents in Hainan carried hantavirus. No patients infected with hantavirus were found in the past. However, the surveillance of HVs-carrying rodents covering the whole territory of Hainan has not stopped.<h4>Methodology/principal findings</h4>For the monitoring of the prevalence of HVs in rodents and the search for theoretical reference for rodent control and HFRS prevention, a total of 60 rodents from 6 monitoring spots were trapped around main ports in Hainan between 2016 and 2019. HV positive samples were identified by a specific kit and sequenced. The data indicated that seven rodents (Rattus norvegicus) were positive for hantavirus with a positivity rate of 11.67%. Phylogenetic analysis suggested that the two complete sequence strains HN1 and HN4 in this research were highly similar to the sequence strains GZRn36 and GZRn148 isolated in Guangdong Province, and they located in the same phylogenetic tree branch which belongs to S2 subtype. Although the two partial sequences HT1 and HT2 isolated in Xisha Islands belong to S2 subtype according to the phylogenetic tree of L segment, they showed a great nucleotide difference with HN1 and HN4. We also found 13 amino acid variations compared with SEOV 80-39 and 6 amino acid mutations related to epitope, and the variations may reduce the effectiveness of the current HFRS vaccines used in humans.<h4>Conclusions/significance</h4>The study indicated HVs carried by rodents found in Hainan Province may be transmitted from Guangdong Province through trading ports and carriage of goods by sea. So it is of great significance to strengthen the surveillance of rodents in port areas especially capture and eliminate rodents on ship. Timely elimination of host animals of hantavirus in port areas is necessary to prevent an outbreak of HVs disease. |
| first_indexed | 2024-03-08T17:04:42Z |
| format | Article |
| id | doaj.art-2d887fad7c0b4d1d9412f897ab32c424 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-08T17:04:42Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-2d887fad7c0b4d1d9412f897ab32c4242024-01-04T05:31:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01173e026485910.1371/journal.pone.0264859Genetic and hosts characterization of hantaviruses in port areas in Hainan Province, P. R. China.Qiu-Wei WangLi TaoSu-Ying LuChang-Qiang ZhuLe-le AiYizhe LuoRong-Bin YuHeng LvYun ZhangChong-Cai WangWei-Long Tan<h4>Background</h4>Hantaviruses (HVs) are major zoonotic pathogens in China that cause hemorrhagic fever with renal syndrome (HFRS) posing a major threat to people's health. Hainan Province, an island located in Southeast China, is an ideal region for sea ports. The unique tropical monsoon climate in Hainan provides sufficient living conditions for rodents, which help spread HVs and other rodent-borne diseases. In the routine monitoring of hantavirus, there was no evidence that rodents in Hainan carried hantavirus. No patients infected with hantavirus were found in the past. However, the surveillance of HVs-carrying rodents covering the whole territory of Hainan has not stopped.<h4>Methodology/principal findings</h4>For the monitoring of the prevalence of HVs in rodents and the search for theoretical reference for rodent control and HFRS prevention, a total of 60 rodents from 6 monitoring spots were trapped around main ports in Hainan between 2016 and 2019. HV positive samples were identified by a specific kit and sequenced. The data indicated that seven rodents (Rattus norvegicus) were positive for hantavirus with a positivity rate of 11.67%. Phylogenetic analysis suggested that the two complete sequence strains HN1 and HN4 in this research were highly similar to the sequence strains GZRn36 and GZRn148 isolated in Guangdong Province, and they located in the same phylogenetic tree branch which belongs to S2 subtype. Although the two partial sequences HT1 and HT2 isolated in Xisha Islands belong to S2 subtype according to the phylogenetic tree of L segment, they showed a great nucleotide difference with HN1 and HN4. We also found 13 amino acid variations compared with SEOV 80-39 and 6 amino acid mutations related to epitope, and the variations may reduce the effectiveness of the current HFRS vaccines used in humans.<h4>Conclusions/significance</h4>The study indicated HVs carried by rodents found in Hainan Province may be transmitted from Guangdong Province through trading ports and carriage of goods by sea. So it is of great significance to strengthen the surveillance of rodents in port areas especially capture and eliminate rodents on ship. Timely elimination of host animals of hantavirus in port areas is necessary to prevent an outbreak of HVs disease.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0264859&type=printable |
| spellingShingle | Qiu-Wei Wang Li Tao Su-Ying Lu Chang-Qiang Zhu Le-le Ai Yizhe Luo Rong-Bin Yu Heng Lv Yun Zhang Chong-Cai Wang Wei-Long Tan Genetic and hosts characterization of hantaviruses in port areas in Hainan Province, P. R. China. PLoS ONE |
| title | Genetic and hosts characterization of hantaviruses in port areas in Hainan Province, P. R. China. |
| title_full | Genetic and hosts characterization of hantaviruses in port areas in Hainan Province, P. R. China. |
| title_fullStr | Genetic and hosts characterization of hantaviruses in port areas in Hainan Province, P. R. China. |
| title_full_unstemmed | Genetic and hosts characterization of hantaviruses in port areas in Hainan Province, P. R. China. |
| title_short | Genetic and hosts characterization of hantaviruses in port areas in Hainan Province, P. R. China. |
| title_sort | genetic and hosts characterization of hantaviruses in port areas in hainan province p r china |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0264859&type=printable |
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