Effect and potential mechanism of oncometabolite succinate promotes distant metastasis of colorectal cancer by activating STAT3
Abstract To investigate the effect of Oncometabolite succinate on colorectal cancer migration and invasion and to initially explore the underlying mechanism.Succinate acid detection kit detected the succinate content in tissues. The growth of colorectal cancer cells was measured by cck-8 assay, woun...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-03-01
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| Series: | BMC Gastroenterology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12876-024-03195-x |
| _version_ | 1797259201188200448 |
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| author | Jiangnan Yu Hong Yang Lin Zhang Suye Ran Qing Shi Pailan Peng Qi Liu Lingyu Song |
| author_facet | Jiangnan Yu Hong Yang Lin Zhang Suye Ran Qing Shi Pailan Peng Qi Liu Lingyu Song |
| author_sort | Jiangnan Yu |
| collection | DOAJ |
| description | Abstract To investigate the effect of Oncometabolite succinate on colorectal cancer migration and invasion and to initially explore the underlying mechanism.Succinate acid detection kit detected the succinate content in tissues. The growth of colorectal cancer cells was measured by cck-8 assay, wound-healing migration assay and transwell migration and invasion assays, and then explored the level of epithelial-mesenchymal transition (EMT) and STAT3/ p-STAT3 expression by western blot analysis and quantitative real-time PCR for mRNA expression. We found that succinate levels were significantly higher in carcinoma tissues than paracancerous tissues. After succinate treatment, the colorectal cancer cell lines SW480 and HCT116 had enhanced migration and invasion, the expression of biomarkers of EMT was promoted, and significantly increased phosphorylation of STAT3. In vivo experiments also showed that succinate can increase p-STAT3 expression, promote the EMT process, and promote the distant metastasis of colorectal cancer in mice.Succinate promotes EMT through the activation of the transcription factor STAT3, thus promoting the migration and invasion of colorectal cancer. |
| first_indexed | 2024-04-24T23:05:40Z |
| format | Article |
| id | doaj.art-2d8902e280a24d38a95c0b4d855d4532 |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-04-24T23:05:40Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-2d8902e280a24d38a95c0b4d855d45322024-03-17T12:27:59ZengBMCBMC Gastroenterology1471-230X2024-03-0124111110.1186/s12876-024-03195-xEffect and potential mechanism of oncometabolite succinate promotes distant metastasis of colorectal cancer by activating STAT3Jiangnan Yu0Hong Yang1Lin Zhang2Suye Ran3Qing Shi4Pailan Peng5Qi Liu6Lingyu Song7Department of Gastroenterology, The First Affiliated Hospital of Sun Yat-sen Medical University Guizhou BranchDepartment of Gastroenterology, Affiliated Hospital of Guizhou Medical UniversityDepartment of Gastroenterology, Affiliated Hospital of Guizhou Medical UniversityDepartment of Gastroenterology, Affiliated Hospital of Guizhou Medical UniversityDepartment of Gastroenterology, Affiliated Hospital of Guizhou Medical UniversityDepartment of Gastroenterology, Affiliated Hospital of Guizhou Medical UniversityDepartment of Gastroenterology, Affiliated Hospital of Guizhou Medical UniversityDepartment of Gastroenterology, Affiliated Hospital of Guizhou Medical UniversityAbstract To investigate the effect of Oncometabolite succinate on colorectal cancer migration and invasion and to initially explore the underlying mechanism.Succinate acid detection kit detected the succinate content in tissues. The growth of colorectal cancer cells was measured by cck-8 assay, wound-healing migration assay and transwell migration and invasion assays, and then explored the level of epithelial-mesenchymal transition (EMT) and STAT3/ p-STAT3 expression by western blot analysis and quantitative real-time PCR for mRNA expression. We found that succinate levels were significantly higher in carcinoma tissues than paracancerous tissues. After succinate treatment, the colorectal cancer cell lines SW480 and HCT116 had enhanced migration and invasion, the expression of biomarkers of EMT was promoted, and significantly increased phosphorylation of STAT3. In vivo experiments also showed that succinate can increase p-STAT3 expression, promote the EMT process, and promote the distant metastasis of colorectal cancer in mice.Succinate promotes EMT through the activation of the transcription factor STAT3, thus promoting the migration and invasion of colorectal cancer.https://doi.org/10.1186/s12876-024-03195-xColorectal cancerSuccinateSTAT3Migration and invasionEpithelial-stromal transition |
| spellingShingle | Jiangnan Yu Hong Yang Lin Zhang Suye Ran Qing Shi Pailan Peng Qi Liu Lingyu Song Effect and potential mechanism of oncometabolite succinate promotes distant metastasis of colorectal cancer by activating STAT3 BMC Gastroenterology Colorectal cancer Succinate STAT3 Migration and invasion Epithelial-stromal transition |
| title | Effect and potential mechanism of oncometabolite succinate promotes distant metastasis of colorectal cancer by activating STAT3 |
| title_full | Effect and potential mechanism of oncometabolite succinate promotes distant metastasis of colorectal cancer by activating STAT3 |
| title_fullStr | Effect and potential mechanism of oncometabolite succinate promotes distant metastasis of colorectal cancer by activating STAT3 |
| title_full_unstemmed | Effect and potential mechanism of oncometabolite succinate promotes distant metastasis of colorectal cancer by activating STAT3 |
| title_short | Effect and potential mechanism of oncometabolite succinate promotes distant metastasis of colorectal cancer by activating STAT3 |
| title_sort | effect and potential mechanism of oncometabolite succinate promotes distant metastasis of colorectal cancer by activating stat3 |
| topic | Colorectal cancer Succinate STAT3 Migration and invasion Epithelial-stromal transition |
| url | https://doi.org/10.1186/s12876-024-03195-x |
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