Treg/IL-17 ratio and Treg differentiation in patients with COPD.
Chronic obstructive pulmonary disease (COPD) is characterized by chronic pulmonary and systematic inflammation. An abnormal adaptive immune response leads to an imbalance between pro- and anti-inflammatory processes. T-helper (Th), T-cytotoxic (Tc) and T-regulatory (Treg) cells may play important ro...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4199736?pdf=render |
| _version_ | 1818129923860070400 |
|---|---|
| author | Yang Jin Yong Wan Gang Chen Long Chen Ming-Qiang Zhang Li Deng Jian-Chu Zhang Xian-Zhi Xiong Jian-Bao Xin |
| author_facet | Yang Jin Yong Wan Gang Chen Long Chen Ming-Qiang Zhang Li Deng Jian-Chu Zhang Xian-Zhi Xiong Jian-Bao Xin |
| author_sort | Yang Jin |
| collection | DOAJ |
| description | Chronic obstructive pulmonary disease (COPD) is characterized by chronic pulmonary and systematic inflammation. An abnormal adaptive immune response leads to an imbalance between pro- and anti-inflammatory processes. T-helper (Th), T-cytotoxic (Tc) and T-regulatory (Treg) cells may play important roles in immune and inflammatory responses. This study was conducted to clarify the changes and imbalance of cytokines and T lymphocyte subsets in patients with COPD, especially during acute exacerbations (AECOPD).Twenty-three patients with stable COPD (SCOPD) and 21 patients with AECOPD were enrolled in the present study. In addition, 20 age-, sex- and weight-matched non-smoking healthy volunteers were included as controls. The serum levels of selected cytokines (TGF-β, IL-10, TNF-α, IL-17 and IL-9) were measured by enzyme-linked immunosorbent assay (ELISA) kits. Furthermore, the T lymphocyte subsets collected from peripheral blood samples were evaluated by flow cytometry after staining with anti-CD3-APC, anti-CD4-PerCP, anti-CD8- PerCP, anti-CD25-FITC and anti-FoxP3-PE monoclonal antibodies. Importantly, to remove the confounding effects of inflammatory factors, the authors introduced a concept of "inflammation adjustment" and corrected each measured value using representative inflammatory markers, such as TNF-α and IL-17.Unlike the other cytokines, serum TGF-β levels were considerably higher in patients with AECOPD relative to the control group regardless of adjustment. There were no significant differences in the percentages of either CD4+ or CD8+ T cells among the three groups. Although Tregs were relatively upregulated during acute exacerbations, their capacities of generation and differentiation were far from sufficient. Finally, the authors noted that the ratios of Treg/IL-17 were similar among groups.These observations suggest that in patients with COPD, especially during acute exacerbations, both pro-inflammatory and anti-inflammatory reactions are strengthened, with the pro-inflammatory reactions dominating. Although the Treg/IL-17 ratios were normal, the regulatory T cells were still insufficient to suppress the accompanying increases in inflammation. All of these changes suggest a complicated mechanism of pro- and anti-inflammatory imbalance which needs to be further investigated. |
| first_indexed | 2024-12-11T07:56:52Z |
| format | Article |
| id | doaj.art-2d8c47948f8f4e84afdc606e19dd6a98 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T07:56:52Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2d8c47948f8f4e84afdc606e19dd6a982022-12-22T01:15:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e11104410.1371/journal.pone.0111044Treg/IL-17 ratio and Treg differentiation in patients with COPD.Yang JinYong WanGang ChenLong ChenMing-Qiang ZhangLi DengJian-Chu ZhangXian-Zhi XiongJian-Bao XinChronic obstructive pulmonary disease (COPD) is characterized by chronic pulmonary and systematic inflammation. An abnormal adaptive immune response leads to an imbalance between pro- and anti-inflammatory processes. T-helper (Th), T-cytotoxic (Tc) and T-regulatory (Treg) cells may play important roles in immune and inflammatory responses. This study was conducted to clarify the changes and imbalance of cytokines and T lymphocyte subsets in patients with COPD, especially during acute exacerbations (AECOPD).Twenty-three patients with stable COPD (SCOPD) and 21 patients with AECOPD were enrolled in the present study. In addition, 20 age-, sex- and weight-matched non-smoking healthy volunteers were included as controls. The serum levels of selected cytokines (TGF-β, IL-10, TNF-α, IL-17 and IL-9) were measured by enzyme-linked immunosorbent assay (ELISA) kits. Furthermore, the T lymphocyte subsets collected from peripheral blood samples were evaluated by flow cytometry after staining with anti-CD3-APC, anti-CD4-PerCP, anti-CD8- PerCP, anti-CD25-FITC and anti-FoxP3-PE monoclonal antibodies. Importantly, to remove the confounding effects of inflammatory factors, the authors introduced a concept of "inflammation adjustment" and corrected each measured value using representative inflammatory markers, such as TNF-α and IL-17.Unlike the other cytokines, serum TGF-β levels were considerably higher in patients with AECOPD relative to the control group regardless of adjustment. There were no significant differences in the percentages of either CD4+ or CD8+ T cells among the three groups. Although Tregs were relatively upregulated during acute exacerbations, their capacities of generation and differentiation were far from sufficient. Finally, the authors noted that the ratios of Treg/IL-17 were similar among groups.These observations suggest that in patients with COPD, especially during acute exacerbations, both pro-inflammatory and anti-inflammatory reactions are strengthened, with the pro-inflammatory reactions dominating. Although the Treg/IL-17 ratios were normal, the regulatory T cells were still insufficient to suppress the accompanying increases in inflammation. All of these changes suggest a complicated mechanism of pro- and anti-inflammatory imbalance which needs to be further investigated.http://europepmc.org/articles/PMC4199736?pdf=render |
| spellingShingle | Yang Jin Yong Wan Gang Chen Long Chen Ming-Qiang Zhang Li Deng Jian-Chu Zhang Xian-Zhi Xiong Jian-Bao Xin Treg/IL-17 ratio and Treg differentiation in patients with COPD. PLoS ONE |
| title | Treg/IL-17 ratio and Treg differentiation in patients with COPD. |
| title_full | Treg/IL-17 ratio and Treg differentiation in patients with COPD. |
| title_fullStr | Treg/IL-17 ratio and Treg differentiation in patients with COPD. |
| title_full_unstemmed | Treg/IL-17 ratio and Treg differentiation in patients with COPD. |
| title_short | Treg/IL-17 ratio and Treg differentiation in patients with COPD. |
| title_sort | treg il 17 ratio and treg differentiation in patients with copd |
| url | http://europepmc.org/articles/PMC4199736?pdf=render |
| work_keys_str_mv | AT yangjin tregil17ratioandtregdifferentiationinpatientswithcopd AT yongwan tregil17ratioandtregdifferentiationinpatientswithcopd AT gangchen tregil17ratioandtregdifferentiationinpatientswithcopd AT longchen tregil17ratioandtregdifferentiationinpatientswithcopd AT mingqiangzhang tregil17ratioandtregdifferentiationinpatientswithcopd AT lideng tregil17ratioandtregdifferentiationinpatientswithcopd AT jianchuzhang tregil17ratioandtregdifferentiationinpatientswithcopd AT xianzhixiong tregil17ratioandtregdifferentiationinpatientswithcopd AT jianbaoxin tregil17ratioandtregdifferentiationinpatientswithcopd |