Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation
Background: Bone resorption due to tooth extraction leads to unpredictable bone volume for future prosthetics. Crab shells were promoted as a solution to prevent bone resorption, along with an effort to reduce biological waste. Purpose: This study aimed to analyze the expression of bone sialoprotein...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2023-06-01
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| Series: | Dental Journal |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/MKG/article/view/36026 |
| _version_ | 1797820355324149760 |
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| author | Michael Josef Kridanto Kamadjaja Sherman Salim Wiwik Herawati Waluyo Tengku Natasha Eleena binti Tengku Ahmad Noor |
| author_facet | Michael Josef Kridanto Kamadjaja Sherman Salim Wiwik Herawati Waluyo Tengku Natasha Eleena binti Tengku Ahmad Noor |
| author_sort | Michael Josef Kridanto Kamadjaja |
| collection | DOAJ |
| description | Background: Bone resorption due to tooth extraction leads to unpredictable bone volume for future prosthetics. Crab shells were promoted as a solution to prevent bone resorption, along with an effort to reduce biological waste. Purpose: This study aimed to analyze the expression of bone sialoprotein (BSP) and dentine matrix protein-1 (DMP1) in the wound healing process in tooth-extraction sockets after applying a crab shell-derived hydroxyapatite scaffold. Methods: The subjects (28 Cavia cobaya) were divided into control and treatment groups. The control group was left untreated, while the treatment group received a hydroxyapatite scaffold of Portunus pelagicus shell in the tooth socket. The expression of BSP and DMP1 was determined by immunohistochemical staining on days 7 and 14. One-way analysis of variance and Tukey’s honest significance difference test were used to find the groups with the most significant difference. Results: The highest mean expression of BSP and DMP1 was in the day 14 treatment group, while the lowest was in the day 7 control group. Conclusion: Administering hydroxyapatite scaffold derived from the Portunus pelagicus shell to the post-extraction sockets increased the expression of both BSP and DMP1. |
| first_indexed | 2024-03-13T09:37:10Z |
| format | Article |
| id | doaj.art-2d8dbf885e8744fa9e392419af7ef860 |
| institution | Directory Open Access Journal |
| issn | 1978-3728 2442-9740 |
| language | English |
| last_indexed | 2024-03-13T09:37:10Z |
| publishDate | 2023-06-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Dental Journal |
| spelling | doaj.art-2d8dbf885e8744fa9e392419af7ef8602023-05-25T06:58:27ZengUniversitas AirlanggaDental Journal1978-37282442-97402023-06-0156210410810.20473/j.djmkg.v56.i2.p104-10834067Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservationMichael Josef Kridanto Kamadjaja0https://orcid.org/0000-0003-3226-7781Sherman Salim1https://orcid.org/0000-0002-6015-586XWiwik Herawati Waluyo2https://orcid.org/0000-0001-7477-3506Tengku Natasha Eleena binti Tengku Ahmad Noor3Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaFaculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaFaculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaMembership of Faculty of Dental Surgery, Royal College of Surgeon, Edinburgh University, United Kingdom; Malaysian Armed Forces Dental Officer, 609 Armed Forces Dental Clinic, Kem Semenggo, Kuching, Serawak, MalaysiaBackground: Bone resorption due to tooth extraction leads to unpredictable bone volume for future prosthetics. Crab shells were promoted as a solution to prevent bone resorption, along with an effort to reduce biological waste. Purpose: This study aimed to analyze the expression of bone sialoprotein (BSP) and dentine matrix protein-1 (DMP1) in the wound healing process in tooth-extraction sockets after applying a crab shell-derived hydroxyapatite scaffold. Methods: The subjects (28 Cavia cobaya) were divided into control and treatment groups. The control group was left untreated, while the treatment group received a hydroxyapatite scaffold of Portunus pelagicus shell in the tooth socket. The expression of BSP and DMP1 was determined by immunohistochemical staining on days 7 and 14. One-way analysis of variance and Tukey’s honest significance difference test were used to find the groups with the most significant difference. Results: The highest mean expression of BSP and DMP1 was in the day 14 treatment group, while the lowest was in the day 7 control group. Conclusion: Administering hydroxyapatite scaffold derived from the Portunus pelagicus shell to the post-extraction sockets increased the expression of both BSP and DMP1.https://e-journal.unair.ac.id/MKG/article/view/36026bspcrab shelldmp1hydroxyapatitemedicine |
| spellingShingle | Michael Josef Kridanto Kamadjaja Sherman Salim Wiwik Herawati Waluyo Tengku Natasha Eleena binti Tengku Ahmad Noor Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation Dental Journal bsp crab shell dmp1 hydroxyapatite medicine |
| title | Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation |
| title_full | Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation |
| title_fullStr | Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation |
| title_full_unstemmed | Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation |
| title_short | Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation |
| title_sort | evaluation of bsp and dmp1 in hydroxyapatite crab shells used for dental socket preservation |
| topic | bsp crab shell dmp1 hydroxyapatite medicine |
| url | https://e-journal.unair.ac.id/MKG/article/view/36026 |
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