Rapid, Label-Free Prediction of Antibiotic Resistance in <i>Salmonella typhimurium</i> by Surface-Enhanced Raman Spectroscopy
The rapid identification of bacterial antibiotic susceptibility is pivotal to the rational administration of antibacterial drugs. In this study, cefotaxime (CTX)-derived resistance in <i>Salmonella typhimurium</i> (abbr. CTX<sup>r</sup>-<i>S. typhimurium</i>) duri...
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/3/1356 |
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| author | Ping Zhang Xi-Hao Wu Lan Su Hui-Qin Wang Tai-Feng Lin Ya-Ping Fang Hui-Min Zhao Wen-Jing Lu Meng-Jia Liu Wen-Bo Liu Da-Wei Zheng |
| author_facet | Ping Zhang Xi-Hao Wu Lan Su Hui-Qin Wang Tai-Feng Lin Ya-Ping Fang Hui-Min Zhao Wen-Jing Lu Meng-Jia Liu Wen-Bo Liu Da-Wei Zheng |
| author_sort | Ping Zhang |
| collection | DOAJ |
| description | The rapid identification of bacterial antibiotic susceptibility is pivotal to the rational administration of antibacterial drugs. In this study, cefotaxime (CTX)-derived resistance in <i>Salmonella typhimurium</i> (abbr. CTX<sup>r</sup>-<i>S. typhimurium</i>) during 3 months of exposure was rapidly recorded using a portable Raman spectrometer. The molecular changes that occurred in the drug-resistant strains were sensitively monitored in whole cells by label-free surface-enhanced Raman scattering (SERS). Various degrees of resistant strains could be accurately discriminated by applying multivariate statistical analyses to bacterial SERS profiles. Minimum inhibitory concentration (MIC) values showed a positive linear correlation with the relative Raman intensities of <i>I</i><sub>990</sub>/<i>I</i><sub>1348</sub>, and the R<sup>2</sup> reached 0.9962. The SERS results were consistent with the data obtained by MIC assays, mutant prevention concentration (MPC) determinations, and Kirby-Bauer antibiotic susceptibility tests (K-B tests). This preliminary proof-of-concept study indicates the high potential of the SERS method to supplement the time-consuming conventional method and help alleviate the challenges of antibiotic resistance in clinical therapy. |
| first_indexed | 2024-03-09T23:48:28Z |
| format | Article |
| id | doaj.art-2d8e3d3916884720917301f85f90f4a4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:48:28Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2d8e3d3916884720917301f85f90f4a42023-11-23T16:39:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-01233135610.3390/ijms23031356Rapid, Label-Free Prediction of Antibiotic Resistance in <i>Salmonella typhimurium</i> by Surface-Enhanced Raman SpectroscopyPing Zhang0Xi-Hao Wu1Lan Su2Hui-Qin Wang3Tai-Feng Lin4Ya-Ping Fang5Hui-Min Zhao6Wen-Jing Lu7Meng-Jia Liu8Wen-Bo Liu9Da-Wei Zheng10Faculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environment and Life, Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology, Beijing 100124, ChinaThe rapid identification of bacterial antibiotic susceptibility is pivotal to the rational administration of antibacterial drugs. In this study, cefotaxime (CTX)-derived resistance in <i>Salmonella typhimurium</i> (abbr. CTX<sup>r</sup>-<i>S. typhimurium</i>) during 3 months of exposure was rapidly recorded using a portable Raman spectrometer. The molecular changes that occurred in the drug-resistant strains were sensitively monitored in whole cells by label-free surface-enhanced Raman scattering (SERS). Various degrees of resistant strains could be accurately discriminated by applying multivariate statistical analyses to bacterial SERS profiles. Minimum inhibitory concentration (MIC) values showed a positive linear correlation with the relative Raman intensities of <i>I</i><sub>990</sub>/<i>I</i><sub>1348</sub>, and the R<sup>2</sup> reached 0.9962. The SERS results were consistent with the data obtained by MIC assays, mutant prevention concentration (MPC) determinations, and Kirby-Bauer antibiotic susceptibility tests (K-B tests). This preliminary proof-of-concept study indicates the high potential of the SERS method to supplement the time-consuming conventional method and help alleviate the challenges of antibiotic resistance in clinical therapy.https://www.mdpi.com/1422-0067/23/3/1356antimicrobial resistancesurface enhanced Raman scatteringrapid detectionlabel-freeminimum inhibitory concentration<i>Salmonella typhimurium</i> |
| spellingShingle | Ping Zhang Xi-Hao Wu Lan Su Hui-Qin Wang Tai-Feng Lin Ya-Ping Fang Hui-Min Zhao Wen-Jing Lu Meng-Jia Liu Wen-Bo Liu Da-Wei Zheng Rapid, Label-Free Prediction of Antibiotic Resistance in <i>Salmonella typhimurium</i> by Surface-Enhanced Raman Spectroscopy International Journal of Molecular Sciences antimicrobial resistance surface enhanced Raman scattering rapid detection label-free minimum inhibitory concentration <i>Salmonella typhimurium</i> |
| title | Rapid, Label-Free Prediction of Antibiotic Resistance in <i>Salmonella typhimurium</i> by Surface-Enhanced Raman Spectroscopy |
| title_full | Rapid, Label-Free Prediction of Antibiotic Resistance in <i>Salmonella typhimurium</i> by Surface-Enhanced Raman Spectroscopy |
| title_fullStr | Rapid, Label-Free Prediction of Antibiotic Resistance in <i>Salmonella typhimurium</i> by Surface-Enhanced Raman Spectroscopy |
| title_full_unstemmed | Rapid, Label-Free Prediction of Antibiotic Resistance in <i>Salmonella typhimurium</i> by Surface-Enhanced Raman Spectroscopy |
| title_short | Rapid, Label-Free Prediction of Antibiotic Resistance in <i>Salmonella typhimurium</i> by Surface-Enhanced Raman Spectroscopy |
| title_sort | rapid label free prediction of antibiotic resistance in i salmonella typhimurium i by surface enhanced raman spectroscopy |
| topic | antimicrobial resistance surface enhanced Raman scattering rapid detection label-free minimum inhibitory concentration <i>Salmonella typhimurium</i> |
| url | https://www.mdpi.com/1422-0067/23/3/1356 |
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