Functional efficiency of PCR vectors in vitro and at the organism level.
The functional efficiency of the expression cassettes integrated into a plasmid and a PCR- amplified fragment was comparatively analyzed after transient transfection in vitro or introduction into the developing embryo of Danio rerio. The cassettes contained the reporter genes, luciferase of Photinus...
Main Authors: | Dina R Safina, Polina I Selina, Marina P Roschina, Maria A Karaseva, Alexey A Komissarov, Ilya V Demidyuk, Eugene D Sverdlov, Sergey V Kostrov |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2020-01-01
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Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0232045 |
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