Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue Engineering
Mg–Zn–Ca bulk metallic glass (BMG) is a promising orthopedic fixation implant because of its biodegradable and biocompatible properties. Structural supporting bone implants with osteoinduction properties for effective bone regeneration have been highly desired in recent years. Osteogenic growth pept...
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MDPI AG
2022-05-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/5/1078 |
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| author | Yun Shin Chu Pei-Chun Wong Jason Shian-Ching Jang Chih-Hwa Chen Si-Han Wu |
| author_facet | Yun Shin Chu Pei-Chun Wong Jason Shian-Ching Jang Chih-Hwa Chen Si-Han Wu |
| author_sort | Yun Shin Chu |
| collection | DOAJ |
| description | Mg–Zn–Ca bulk metallic glass (BMG) is a promising orthopedic fixation implant because of its biodegradable and biocompatible properties. Structural supporting bone implants with osteoinduction properties for effective bone regeneration have been highly desired in recent years. Osteogenic growth peptide (OGP) can increase the proliferation and differentiation of mesenchymal stem cells and enhance the mineralization of osteoblast cells. However, the short half-life and non-specificity to target areas limit applications of OGP. Mesoporous silica nanoparticles (MSNs) as nanocarriers possess excellent properties, such as easy surface modification, superior targeting efficiency, and high loading capacity of drugs or proteins. Accordingly, we propose a system of combining the OGP-containing MSNs with Mg–Zn–Ca BMG materials to promote bone regeneration. In this work, we conjugated cysteine-containing OGP (cgOGP, 16 a.a.) to interior walls of channels in MSNs and maintained the dispersity of MSNs via PEGylation. An in vitro study showed that metal ions released from Mg–Zn–Ca BMG promoted cell proliferation and migration and elevated alkaline phosphatase (ALP) activity and mineralization. On treating cells with both BMG ion-containing Minimum Essential Medium Eagle-alpha modification (α-MEM) and OGP-conjugated MSNs, enhanced focal adhesion turnover and promoted differentiation were observed. Hematological analyses showed the biocompatible nature of this BMG/nanocomposite system. In addition, in vivo micro-computed tomographic and histological observations revealed that our system stimulated osteogenesis and new bone formation around the implant site. |
| first_indexed | 2024-03-10T03:05:25Z |
| format | Article |
| id | doaj.art-2d967c47e172496dbbd6ed72f3675785 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T03:05:25Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-2d967c47e172496dbbd6ed72f36757852023-11-23T12:39:15ZengMDPI AGPharmaceutics1999-49232022-05-01145107810.3390/pharmaceutics14051078Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue EngineeringYun Shin Chu0Pei-Chun Wong1Jason Shian-Ching Jang2Chih-Hwa Chen3Si-Han Wu4Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 11031, TaiwanDepartment of Orthopedics, Taipei Medical University Hospital, Taipei 11031, TaiwanGraduate Institute of Materials Science and Engineering, National Central University, Taoyuan 32001, TaiwanSchool of Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, TaiwanGraduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 11031, TaiwanMg–Zn–Ca bulk metallic glass (BMG) is a promising orthopedic fixation implant because of its biodegradable and biocompatible properties. Structural supporting bone implants with osteoinduction properties for effective bone regeneration have been highly desired in recent years. Osteogenic growth peptide (OGP) can increase the proliferation and differentiation of mesenchymal stem cells and enhance the mineralization of osteoblast cells. However, the short half-life and non-specificity to target areas limit applications of OGP. Mesoporous silica nanoparticles (MSNs) as nanocarriers possess excellent properties, such as easy surface modification, superior targeting efficiency, and high loading capacity of drugs or proteins. Accordingly, we propose a system of combining the OGP-containing MSNs with Mg–Zn–Ca BMG materials to promote bone regeneration. In this work, we conjugated cysteine-containing OGP (cgOGP, 16 a.a.) to interior walls of channels in MSNs and maintained the dispersity of MSNs via PEGylation. An in vitro study showed that metal ions released from Mg–Zn–Ca BMG promoted cell proliferation and migration and elevated alkaline phosphatase (ALP) activity and mineralization. On treating cells with both BMG ion-containing Minimum Essential Medium Eagle-alpha modification (α-MEM) and OGP-conjugated MSNs, enhanced focal adhesion turnover and promoted differentiation were observed. Hematological analyses showed the biocompatible nature of this BMG/nanocomposite system. In addition, in vivo micro-computed tomographic and histological observations revealed that our system stimulated osteogenesis and new bone formation around the implant site.https://www.mdpi.com/1999-4923/14/5/1078mesoporous silica nanoparticlesMg–Zn–Ca bulk metallic glassbone tissue engineeringosteogenic growth peptideosteogenic differentiationosteoinduction |
| spellingShingle | Yun Shin Chu Pei-Chun Wong Jason Shian-Ching Jang Chih-Hwa Chen Si-Han Wu Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue Engineering Pharmaceutics mesoporous silica nanoparticles Mg–Zn–Ca bulk metallic glass bone tissue engineering osteogenic growth peptide osteogenic differentiation osteoinduction |
| title | Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue Engineering |
| title_full | Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue Engineering |
| title_fullStr | Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue Engineering |
| title_full_unstemmed | Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue Engineering |
| title_short | Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue Engineering |
| title_sort | combining mg zn ca bulk metallic glass with a mesoporous silica nanocomposite for bone tissue engineering |
| topic | mesoporous silica nanoparticles Mg–Zn–Ca bulk metallic glass bone tissue engineering osteogenic growth peptide osteogenic differentiation osteoinduction |
| url | https://www.mdpi.com/1999-4923/14/5/1078 |
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