Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse.
The sensitivity of the double agar gel immunodiffusion test is about 90% in patients with untreated paracoccidioidomycosis (PCM), but it is much lower in cases of relapse. In addition, serum from patients with PCM caused by Paracoccidioides lutzii, frequent in the Midwest region of Brazil, do not re...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6114792?pdf=render |
| _version_ | 1819104509974020096 |
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| author | Tatiane Fernanda Sylvestre Ricardo de Souza Cavalcante Julhiany de Fátima da Silva Anamaria Mello Miranda Paniago Simone Schneider Weber Bianca Alves Pauletti Lídia Raquel de Carvalho Lucilene Delazari Dos Santos Rinaldo Poncio Mendes |
| author_facet | Tatiane Fernanda Sylvestre Ricardo de Souza Cavalcante Julhiany de Fátima da Silva Anamaria Mello Miranda Paniago Simone Schneider Weber Bianca Alves Pauletti Lídia Raquel de Carvalho Lucilene Delazari Dos Santos Rinaldo Poncio Mendes |
| author_sort | Tatiane Fernanda Sylvestre |
| collection | DOAJ |
| description | The sensitivity of the double agar gel immunodiffusion test is about 90% in patients with untreated paracoccidioidomycosis (PCM), but it is much lower in cases of relapse. In addition, serum from patients with PCM caused by Paracoccidioides lutzii, frequent in the Midwest region of Brazil, do not react with the classical antigen obtained from Pb B-339. These findings showed the need for alternative diagnostic methods, such as biological markers through proteomics. The aim of this study was to identify biomarkers for the safe identification of PCM relapse and specific proteins that could distinguish infections caused by Paracoccidioides brasiliensis from those produced by Paracoccidioides lutzii. Proteomic analysis was performed in serum from 9 patients with PCM caused by P. brasiliensis, with and without relapse, from 4 patients with PCM produced by P. lutzii, and from 3 healthy controls. The comparative evaluation of the 29 identified plasma proteins suggested that the presence of the immunoglobulin (Ig) alpha-2 chain C region and the absence of Ig heavy chain V-III TIL indicate infection by P. lutzii. In addition, the absence of complement factor B protein might be a predictor of relapse. The evaluation of these proteins in a higher number of patients should be carried out in order to validate these findings. |
| first_indexed | 2024-12-22T02:07:30Z |
| format | Article |
| id | doaj.art-2d97f62ba5c349bd82914a6c97337ddb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T02:07:30Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2d97f62ba5c349bd82914a6c97337ddb2022-12-21T18:42:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01138e020280410.1371/journal.pone.0202804Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse.Tatiane Fernanda SylvestreRicardo de Souza CavalcanteJulhiany de Fátima da SilvaAnamaria Mello Miranda PaniagoSimone Schneider WeberBianca Alves PaulettiLídia Raquel de CarvalhoLucilene Delazari Dos SantosRinaldo Poncio MendesThe sensitivity of the double agar gel immunodiffusion test is about 90% in patients with untreated paracoccidioidomycosis (PCM), but it is much lower in cases of relapse. In addition, serum from patients with PCM caused by Paracoccidioides lutzii, frequent in the Midwest region of Brazil, do not react with the classical antigen obtained from Pb B-339. These findings showed the need for alternative diagnostic methods, such as biological markers through proteomics. The aim of this study was to identify biomarkers for the safe identification of PCM relapse and specific proteins that could distinguish infections caused by Paracoccidioides brasiliensis from those produced by Paracoccidioides lutzii. Proteomic analysis was performed in serum from 9 patients with PCM caused by P. brasiliensis, with and without relapse, from 4 patients with PCM produced by P. lutzii, and from 3 healthy controls. The comparative evaluation of the 29 identified plasma proteins suggested that the presence of the immunoglobulin (Ig) alpha-2 chain C region and the absence of Ig heavy chain V-III TIL indicate infection by P. lutzii. In addition, the absence of complement factor B protein might be a predictor of relapse. The evaluation of these proteins in a higher number of patients should be carried out in order to validate these findings.http://europepmc.org/articles/PMC6114792?pdf=render |
| spellingShingle | Tatiane Fernanda Sylvestre Ricardo de Souza Cavalcante Julhiany de Fátima da Silva Anamaria Mello Miranda Paniago Simone Schneider Weber Bianca Alves Pauletti Lídia Raquel de Carvalho Lucilene Delazari Dos Santos Rinaldo Poncio Mendes Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse. PLoS ONE |
| title | Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse. |
| title_full | Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse. |
| title_fullStr | Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse. |
| title_full_unstemmed | Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse. |
| title_short | Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse. |
| title_sort | serological proteomic biomarkers to identify paracoccidioides species and risk of relapse |
| url | http://europepmc.org/articles/PMC6114792?pdf=render |
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