The beneficial effects of spraying of probiotic Bacillus and Lactobacillus bacteria on broiler chickens experimentally infected with avian influenza virus H9N2
ABSTRACT: This study investigated the clinical, antiviral, and immunological effects of spraying Bacillus spp. and Lactobacillus spp. as a single or mixture probiotic compound on experimentally infected broiler chickens with AIV H9N2. Two hundred and forty 1-day-old broilers were randomly assigned t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912300192X |
| _version_ | 1797802295816093696 |
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| author | Dana Rasaei Seyedeh Alemeh Hosseinian keramat Asasi Seyed Shahram Shekarforoush Azizollah Khodakaram-Tafti |
| author_facet | Dana Rasaei Seyedeh Alemeh Hosseinian keramat Asasi Seyed Shahram Shekarforoush Azizollah Khodakaram-Tafti |
| author_sort | Dana Rasaei |
| collection | DOAJ |
| description | ABSTRACT: This study investigated the clinical, antiviral, and immunological effects of spraying Bacillus spp. and Lactobacillus spp. as a single or mixture probiotic compound on experimentally infected broiler chickens with AIV H9N2. Two hundred and forty 1-day-old broilers were randomly assigned to 6 groups as follows: Ctrl− (no challenge AIV; no spray probiotic), Ctrl+ (AIV challenged; no spray probiotic), AI+B (AIV challenged; daily spraying of probiotic Bacillus spp.), AI+L group (AIV challenged; daily spraying of probiotic Lactobacillus spp.), AIV+BL (AIV challenged; daily spraying of probiotic Bacillus spp. and Lactobacillus spp.), and G-DW (daily spraying of normal saline; no AIV challenged). The birds were reared for 35 d. On the 22nd day of age, broiler chickens were challenged by AIV H9N2. The probiotics were sprayed at 9×109 CFU/m2 daily for 35 d. Growth performance, clinical signs, virus shedding, macroscopic and microscopic lesions were evaluated at various days in all groups. Spraying with probiotics improved the body weight gain and food conversion ratio in the AI+B, AI+L, and AI+BL groups compared to the Ctrl+. The severity of clinical signs, gross lesions, pathological lesions and viral shedding in the probiotic treatment groups was lower than in the Ctrl+ group. The findings of this study suggest the daily spraying of Lactobacillus and Bacillus probiotics alone or in combination during the rearing period reduce clinical and nonclinical aspects of H9N2 virus infection; so, it can be effective as a preventive protocol for controlling the severity of AIV H9N2 infection in broilers. |
| first_indexed | 2024-03-13T05:03:33Z |
| format | Article |
| id | doaj.art-2d9803e12cd645608962cb55416f3544 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-13T05:03:33Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-2d9803e12cd645608962cb55416f35442023-06-17T05:17:15ZengElsevierPoultry Science0032-57912023-07-011027102669The beneficial effects of spraying of probiotic Bacillus and Lactobacillus bacteria on broiler chickens experimentally infected with avian influenza virus H9N2Dana Rasaei0Seyedeh Alemeh Hosseinian1keramat Asasi2Seyed Shahram Shekarforoush3Azizollah Khodakaram-Tafti4Avian Diseases Research Center, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, IranAvian Diseases Research Center, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran; Corresponding author:Avian Diseases Research Center, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, IranDepartment of Food Hygiene, School of Veterinary Medicine, Shiraz University, Shiraz, IranDepartment of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, IranABSTRACT: This study investigated the clinical, antiviral, and immunological effects of spraying Bacillus spp. and Lactobacillus spp. as a single or mixture probiotic compound on experimentally infected broiler chickens with AIV H9N2. Two hundred and forty 1-day-old broilers were randomly assigned to 6 groups as follows: Ctrl− (no challenge AIV; no spray probiotic), Ctrl+ (AIV challenged; no spray probiotic), AI+B (AIV challenged; daily spraying of probiotic Bacillus spp.), AI+L group (AIV challenged; daily spraying of probiotic Lactobacillus spp.), AIV+BL (AIV challenged; daily spraying of probiotic Bacillus spp. and Lactobacillus spp.), and G-DW (daily spraying of normal saline; no AIV challenged). The birds were reared for 35 d. On the 22nd day of age, broiler chickens were challenged by AIV H9N2. The probiotics were sprayed at 9×109 CFU/m2 daily for 35 d. Growth performance, clinical signs, virus shedding, macroscopic and microscopic lesions were evaluated at various days in all groups. Spraying with probiotics improved the body weight gain and food conversion ratio in the AI+B, AI+L, and AI+BL groups compared to the Ctrl+. The severity of clinical signs, gross lesions, pathological lesions and viral shedding in the probiotic treatment groups was lower than in the Ctrl+ group. The findings of this study suggest the daily spraying of Lactobacillus and Bacillus probiotics alone or in combination during the rearing period reduce clinical and nonclinical aspects of H9N2 virus infection; so, it can be effective as a preventive protocol for controlling the severity of AIV H9N2 infection in broilers.http://www.sciencedirect.com/science/article/pii/S003257912300192XBacillus spp.Lactobacillus spp.probiotic spraybroilerclinical damage |
| spellingShingle | Dana Rasaei Seyedeh Alemeh Hosseinian keramat Asasi Seyed Shahram Shekarforoush Azizollah Khodakaram-Tafti The beneficial effects of spraying of probiotic Bacillus and Lactobacillus bacteria on broiler chickens experimentally infected with avian influenza virus H9N2 Poultry Science Bacillus spp. Lactobacillus spp. probiotic spray broiler clinical damage |
| title | The beneficial effects of spraying of probiotic Bacillus and Lactobacillus bacteria on broiler chickens experimentally infected with avian influenza virus H9N2 |
| title_full | The beneficial effects of spraying of probiotic Bacillus and Lactobacillus bacteria on broiler chickens experimentally infected with avian influenza virus H9N2 |
| title_fullStr | The beneficial effects of spraying of probiotic Bacillus and Lactobacillus bacteria on broiler chickens experimentally infected with avian influenza virus H9N2 |
| title_full_unstemmed | The beneficial effects of spraying of probiotic Bacillus and Lactobacillus bacteria on broiler chickens experimentally infected with avian influenza virus H9N2 |
| title_short | The beneficial effects of spraying of probiotic Bacillus and Lactobacillus bacteria on broiler chickens experimentally infected with avian influenza virus H9N2 |
| title_sort | beneficial effects of spraying of probiotic bacillus and lactobacillus bacteria on broiler chickens experimentally infected with avian influenza virus h9n2 |
| topic | Bacillus spp. Lactobacillus spp. probiotic spray broiler clinical damage |
| url | http://www.sciencedirect.com/science/article/pii/S003257912300192X |
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