Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro
Diabetes, a chronic group of medical disorders characterized byhyperglycemia, has become a global pandemic. Some hormones may influence the course and outcome of diabetes, especially if they potentiate the formation of reactive oxygen species (ROS). There is a close relationship between thyroid diso...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9072 |
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| author | Ninoslav Djelić Sunčica Borozan Vesna Dimitrijević-Srećković Nevena Pajović Milorad Mirilović Helga Stopper Zoran Stanimirović |
| author_facet | Ninoslav Djelić Sunčica Borozan Vesna Dimitrijević-Srećković Nevena Pajović Milorad Mirilović Helga Stopper Zoran Stanimirović |
| author_sort | Ninoslav Djelić |
| collection | DOAJ |
| description | Diabetes, a chronic group of medical disorders characterized byhyperglycemia, has become a global pandemic. Some hormones may influence the course and outcome of diabetes, especially if they potentiate the formation of reactive oxygen species (ROS). There is a close relationship between thyroid disorders and diabetes. The main objective of this investigation was to find out whether peripheral blood mononuclear cells (PBMCs) are more prone to DNA damage by triiodothyronine (T<sub>3</sub>) (0.1, 1 and 10 μM) at various stages of progression through diabetes (obese, prediabetics, and type 2 diabetes mellitus—T2DM persons). In addition, some biochemical parameters of oxidative stress (catalase-CAT, thiobarbituric acid reactive substances—TBARS) and lactate dehydrogenase (LDH) were evaluated. PBMCs from prediabetic and diabetic patients exhibited increased sensitivity for T<sub>3</sub> regarding elevated level of DNA damage, inhibition of catalase, and increase of TBARS and LDH. PBMCs from obese patients reacted in the same manner, except for DNA damage. The results of this study should contribute to a better understanding of the role of thyroid hormones in the progression of T2DM. |
| first_indexed | 2024-03-09T04:19:52Z |
| format | Article |
| id | doaj.art-2d99b1eefb464dfda29c58678469df32 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:19:52Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2d99b1eefb464dfda29c58678469df322023-12-03T13:48:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316907210.3390/ijms23169072Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In VitroNinoslav Djelić0Sunčica Borozan1Vesna Dimitrijević-Srećković2Nevena Pajović3Milorad Mirilović4Helga Stopper5Zoran Stanimirović6Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, SerbiaDepartment of Chemistry, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, SerbiaClinic for Endocrinology, Diabetes and Metabolic Diseases, Clinical Center of Serbia, 11000 Belgrade, SerbiaClinic for Endocrinology, Diabetes and Metabolic Diseases, Clinical Center of Serbia, 11000 Belgrade, SerbiaDepartment of Economics and Statistics, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, SerbiaInstitute of Pharmacology and Toxicology, University of Würzburg, 97070 Würzburg, GermanyDepartment of Biology, Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, SerbiaDiabetes, a chronic group of medical disorders characterized byhyperglycemia, has become a global pandemic. Some hormones may influence the course and outcome of diabetes, especially if they potentiate the formation of reactive oxygen species (ROS). There is a close relationship between thyroid disorders and diabetes. The main objective of this investigation was to find out whether peripheral blood mononuclear cells (PBMCs) are more prone to DNA damage by triiodothyronine (T<sub>3</sub>) (0.1, 1 and 10 μM) at various stages of progression through diabetes (obese, prediabetics, and type 2 diabetes mellitus—T2DM persons). In addition, some biochemical parameters of oxidative stress (catalase-CAT, thiobarbituric acid reactive substances—TBARS) and lactate dehydrogenase (LDH) were evaluated. PBMCs from prediabetic and diabetic patients exhibited increased sensitivity for T<sub>3</sub> regarding elevated level of DNA damage, inhibition of catalase, and increase of TBARS and LDH. PBMCs from obese patients reacted in the same manner, except for DNA damage. The results of this study should contribute to a better understanding of the role of thyroid hormones in the progression of T2DM.https://www.mdpi.com/1422-0067/23/16/9072diabetesoxidative stressDNA damagelymphocytesthyroid hormone |
| spellingShingle | Ninoslav Djelić Sunčica Borozan Vesna Dimitrijević-Srećković Nevena Pajović Milorad Mirilović Helga Stopper Zoran Stanimirović Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro International Journal of Molecular Sciences diabetes oxidative stress DNA damage lymphocytes thyroid hormone |
| title | Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro |
| title_full | Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro |
| title_fullStr | Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro |
| title_full_unstemmed | Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro |
| title_short | Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro |
| title_sort | oxidative stress and dna damage in peripheral blood mononuclear cells from normal obese prediabetic and diabetic persons exposed to thyroid hormone in vitro |
| topic | diabetes oxidative stress DNA damage lymphocytes thyroid hormone |
| url | https://www.mdpi.com/1422-0067/23/16/9072 |
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