Protein expression profile changes of lung tissue in patients with pulmonary hypertension
Background Pulmonary hypertension occurs in approximately 1% of the global population, and the prognosis for such patients may be poor. However, the mechanisms underlying the development of this disease remain unclear. Thus, understanding the development of pulmonary hypertension and finding new the...
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PeerJ Inc.
2020-01-01
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| _version_ | 1797419154209243136 |
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| author | Min Wu Yijin Wu Jinsong Huang Yueheng Wu Hongmei Wu Benyuan Jiang Jian Zhuang |
| author_facet | Min Wu Yijin Wu Jinsong Huang Yueheng Wu Hongmei Wu Benyuan Jiang Jian Zhuang |
| author_sort | Min Wu |
| collection | DOAJ |
| description | Background Pulmonary hypertension occurs in approximately 1% of the global population, and the prognosis for such patients may be poor. However, the mechanisms underlying the development of this disease remain unclear. Thus, understanding the development of pulmonary hypertension and finding new therapeutic targets and approaches are important for improved clinical outcomes. Methods Lung tissue specimens were collected from six patients with atrial septal defect and pulmonary hypertension (all women, with a mean age of 46.5 ± 4.7 years, and their condition could not be corrected with an internal medical occlusion device) and from nine control patients with lung cancer who underwent lobectomy (six men and three women, with a mean age of 56.7 ± 1.7 years). Isobaric tags for relative and absolute quantitation and liquid chromatography tandem mass spectrometry analyses were used to detect protein expression levels. Results We found 74 significantly upregulated and 88 significantly downregulated differentially expressed proteins between control and pulmonary hypertensive lung tissue specimens. Gene ontology analyses identified the top 20 terms in all three categories, that is, biological process, cellular component, and molecular function. Kyoto Encyclopedia of Genes and Genomes and protein–protein interaction analyses determined the top 10 signaling pathways and found that the six hub proteins associated with the differentially expressed upregulated proteins (PRKAA1, DHPR, ACTB, desmin, ACTG1, and ITGA1) were all involved in hypertrophic cardiomyopathy, arrhythmogenic right ventricular cardiomyopathy, and dilated cardiomyopathy. Conclusion Our results identified protein expression profile changes in lung tissue derived from patients with pulmonary hypertension, providing potential new biomarkers for clinical diagnosis and prognosis for patients with pulmonary hypertension and offering candidate protein targets for future therapeutic drug development. |
| first_indexed | 2024-03-09T06:44:06Z |
| format | Article |
| id | doaj.art-2d9d6f1193bf49eb8829f330f2a98e95 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:44:06Z |
| publishDate | 2020-01-01 |
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| spelling | doaj.art-2d9d6f1193bf49eb8829f330f2a98e952023-12-03T10:40:52ZengPeerJ Inc.PeerJ2167-83592020-01-018e815310.7717/peerj.8153Protein expression profile changes of lung tissue in patients with pulmonary hypertensionMin Wu0Yijin Wu1Jinsong Huang2Yueheng Wu3Hongmei Wu4Benyuan Jiang5Jian Zhuang6Department of Cardiac Surgery, Guangdong Provincial People’s Hospital, Guangzhou City, Guangdong Province, ChinaDepartment of Cardiac Surgery, Guangdong Provincial People’s Hospital, Guangzhou City, Guangdong Province, ChinaDepartment of Cardiac Surgery, Guangdong Provincial People’s Hospital, Guangzhou City, Guangdong Province, ChinaDepartment of Cardiac Surgery, Guangdong Provincial People’s Hospital, Guangzhou City, Guangdong Province, ChinaDepartment of Cardiac Surgery, Guangdong Provincial People’s Hospital, Guangzhou City, Guangdong Province, ChinaDepartment of Cardiac Surgery, Guangdong Provincial People’s Hospital, Guangzhou City, Guangdong Province, ChinaDepartment of Cardiac Surgery, Guangdong Provincial People’s Hospital, Guangzhou City, Guangdong Province, ChinaBackground Pulmonary hypertension occurs in approximately 1% of the global population, and the prognosis for such patients may be poor. However, the mechanisms underlying the development of this disease remain unclear. Thus, understanding the development of pulmonary hypertension and finding new therapeutic targets and approaches are important for improved clinical outcomes. Methods Lung tissue specimens were collected from six patients with atrial septal defect and pulmonary hypertension (all women, with a mean age of 46.5 ± 4.7 years, and their condition could not be corrected with an internal medical occlusion device) and from nine control patients with lung cancer who underwent lobectomy (six men and three women, with a mean age of 56.7 ± 1.7 years). Isobaric tags for relative and absolute quantitation and liquid chromatography tandem mass spectrometry analyses were used to detect protein expression levels. Results We found 74 significantly upregulated and 88 significantly downregulated differentially expressed proteins between control and pulmonary hypertensive lung tissue specimens. Gene ontology analyses identified the top 20 terms in all three categories, that is, biological process, cellular component, and molecular function. Kyoto Encyclopedia of Genes and Genomes and protein–protein interaction analyses determined the top 10 signaling pathways and found that the six hub proteins associated with the differentially expressed upregulated proteins (PRKAA1, DHPR, ACTB, desmin, ACTG1, and ITGA1) were all involved in hypertrophic cardiomyopathy, arrhythmogenic right ventricular cardiomyopathy, and dilated cardiomyopathy. Conclusion Our results identified protein expression profile changes in lung tissue derived from patients with pulmonary hypertension, providing potential new biomarkers for clinical diagnosis and prognosis for patients with pulmonary hypertension and offering candidate protein targets for future therapeutic drug development.https://peerj.com/articles/8153.pdfPulmonary hypertensionProteomicsLC–MS/MSiTRAQPRKAA1DHPR |
| spellingShingle | Min Wu Yijin Wu Jinsong Huang Yueheng Wu Hongmei Wu Benyuan Jiang Jian Zhuang Protein expression profile changes of lung tissue in patients with pulmonary hypertension PeerJ Pulmonary hypertension Proteomics LC–MS/MS iTRAQ PRKAA1 DHPR |
| title | Protein expression profile changes of lung tissue in patients with pulmonary hypertension |
| title_full | Protein expression profile changes of lung tissue in patients with pulmonary hypertension |
| title_fullStr | Protein expression profile changes of lung tissue in patients with pulmonary hypertension |
| title_full_unstemmed | Protein expression profile changes of lung tissue in patients with pulmonary hypertension |
| title_short | Protein expression profile changes of lung tissue in patients with pulmonary hypertension |
| title_sort | protein expression profile changes of lung tissue in patients with pulmonary hypertension |
| topic | Pulmonary hypertension Proteomics LC–MS/MS iTRAQ PRKAA1 DHPR |
| url | https://peerj.com/articles/8153.pdf |
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