Supercritical Impregnation of Mesoglycan and Lactoferrin on Polyurethane Electrospun Fibers for Wound Healing Applications
Fibrous membranes of thermoplastic polyurethane (TPU) were fabricated through a uni-axial electrospinning process. Fibers were then separately charged with two pharmacological agents, mesoglycan (MSG) and lactoferrin (LF), by supercritical CO<sub>2</sub> impregnation. Scanning Electron M...
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| author | Stefania Mottola Gianluca Viscusi Giovanna Iannone Raffaella Belvedere Antonello Petrella Iolanda De Marco Giuliana Gorrasi |
| author_facet | Stefania Mottola Gianluca Viscusi Giovanna Iannone Raffaella Belvedere Antonello Petrella Iolanda De Marco Giuliana Gorrasi |
| author_sort | Stefania Mottola |
| collection | DOAJ |
| description | Fibrous membranes of thermoplastic polyurethane (TPU) were fabricated through a uni-axial electrospinning process. Fibers were then separately charged with two pharmacological agents, mesoglycan (MSG) and lactoferrin (LF), by supercritical CO<sub>2</sub> impregnation. Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS) analysis proved the formation of a micrometric structure with a homogeneous distribution of mesoglycan and lactoferrin. Besides, the degree of retention is calculated in four liquid media with different pHs. At the same time, angle contact analysis proved the formation of a hydrophobic membrane loaded with MSG and a hydrophilic LF-loaded one. The impregnation kinetics demonstrated a maximum loaded amount equal to 0.18 ± 0.20% and 0.07 ± 0.05% for MSG and LT, respectively. In vitro tests were performed using a Franz diffusion cell to simulate the contact with the human skin. The release of MSG reaches a plateau after about 28 h while LF release leveled off after 15 h. The in vitro compatibility of electrospun membranes has been evaluated on HaCaT and BJ cell lines, as human keratinocytes and fibroblasts, respectively. The reported data proved the potential application of fabricated membranes for wound healing. |
| first_indexed | 2024-03-11T03:05:26Z |
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| id | doaj.art-2d9ed8f9cf934773861c7119bbd2de78 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:05:26Z |
| publishDate | 2023-05-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2d9ed8f9cf934773861c7119bbd2de782023-11-18T07:56:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012411926910.3390/ijms24119269Supercritical Impregnation of Mesoglycan and Lactoferrin on Polyurethane Electrospun Fibers for Wound Healing ApplicationsStefania Mottola0Gianluca Viscusi1Giovanna Iannone2Raffaella Belvedere3Antonello Petrella4Iolanda De Marco5Giuliana Gorrasi6Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, ItalyDepartment of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, ItalyDepartment of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, ItalyDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, ItalyDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, ItalyDepartment of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, ItalyDepartment of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, ItalyFibrous membranes of thermoplastic polyurethane (TPU) were fabricated through a uni-axial electrospinning process. Fibers were then separately charged with two pharmacological agents, mesoglycan (MSG) and lactoferrin (LF), by supercritical CO<sub>2</sub> impregnation. Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS) analysis proved the formation of a micrometric structure with a homogeneous distribution of mesoglycan and lactoferrin. Besides, the degree of retention is calculated in four liquid media with different pHs. At the same time, angle contact analysis proved the formation of a hydrophobic membrane loaded with MSG and a hydrophilic LF-loaded one. The impregnation kinetics demonstrated a maximum loaded amount equal to 0.18 ± 0.20% and 0.07 ± 0.05% for MSG and LT, respectively. In vitro tests were performed using a Franz diffusion cell to simulate the contact with the human skin. The release of MSG reaches a plateau after about 28 h while LF release leveled off after 15 h. The in vitro compatibility of electrospun membranes has been evaluated on HaCaT and BJ cell lines, as human keratinocytes and fibroblasts, respectively. The reported data proved the potential application of fabricated membranes for wound healing.https://www.mdpi.com/1422-0067/24/11/9269supercritical carbon dioxideelectrospinningtransdermal drug deliveryrelease tests modelingcytotoxicity tests |
| spellingShingle | Stefania Mottola Gianluca Viscusi Giovanna Iannone Raffaella Belvedere Antonello Petrella Iolanda De Marco Giuliana Gorrasi Supercritical Impregnation of Mesoglycan and Lactoferrin on Polyurethane Electrospun Fibers for Wound Healing Applications International Journal of Molecular Sciences supercritical carbon dioxide electrospinning transdermal drug delivery release tests modeling cytotoxicity tests |
| title | Supercritical Impregnation of Mesoglycan and Lactoferrin on Polyurethane Electrospun Fibers for Wound Healing Applications |
| title_full | Supercritical Impregnation of Mesoglycan and Lactoferrin on Polyurethane Electrospun Fibers for Wound Healing Applications |
| title_fullStr | Supercritical Impregnation of Mesoglycan and Lactoferrin on Polyurethane Electrospun Fibers for Wound Healing Applications |
| title_full_unstemmed | Supercritical Impregnation of Mesoglycan and Lactoferrin on Polyurethane Electrospun Fibers for Wound Healing Applications |
| title_short | Supercritical Impregnation of Mesoglycan and Lactoferrin on Polyurethane Electrospun Fibers for Wound Healing Applications |
| title_sort | supercritical impregnation of mesoglycan and lactoferrin on polyurethane electrospun fibers for wound healing applications |
| topic | supercritical carbon dioxide electrospinning transdermal drug delivery release tests modeling cytotoxicity tests |
| url | https://www.mdpi.com/1422-0067/24/11/9269 |
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