Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor
A novel matrix composed of chitosan-graft-polyaniline (CHIT-g-PANI) was electrochemically prepared to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-g-PANI matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-g-PANI b...
| Format: | Article |
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| Language: | English |
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Budapest University of Technology
2009-09-01
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| Series: | eXPRESS Polymer Letters |
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| Online Access: | http://www.expresspolymlett.com/letolt.php?file=EPL-0001053&mi=cd |
| _version_ | 1818057136077275136 |
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| collection | DOAJ |
| description | A novel matrix composed of chitosan-graft-polyaniline (CHIT-g-PANI) was electrochemically prepared to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-g-PANI matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-g-PANI biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), contact angle measurement and cyclic voltammetry (CV) taking CHIT-g-PANI as a reference. The influence of various parameters on CAH enzyme activity within the matrix was investigated including pH, temperature, and time. The Michaelis-Menten constant and apparent activities for the CAH enzyme were calculated to be 0.51 mM and 83.59 mg/cm2, respectively; indicating CHIT-g-PANI matrix has a high affinity to immobilize CAH enzyme. |
| first_indexed | 2024-12-10T12:39:56Z |
| format | Article |
| id | doaj.art-2da0ef01e8974368b53c877e90f6a728 |
| institution | Directory Open Access Journal |
| issn | 1788-618X |
| language | English |
| last_indexed | 2024-12-10T12:39:56Z |
| publishDate | 2009-09-01 |
| publisher | Budapest University of Technology |
| record_format | Article |
| series | eXPRESS Polymer Letters |
| spelling | doaj.art-2da0ef01e8974368b53c877e90f6a7282022-12-22T01:48:33ZengBudapest University of TechnologyeXPRESS Polymer Letters1788-618X2009-09-013955355910.3144/expresspolymlett.2009.69Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensorA novel matrix composed of chitosan-graft-polyaniline (CHIT-g-PANI) was electrochemically prepared to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-g-PANI matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-g-PANI biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), contact angle measurement and cyclic voltammetry (CV) taking CHIT-g-PANI as a reference. The influence of various parameters on CAH enzyme activity within the matrix was investigated including pH, temperature, and time. The Michaelis-Menten constant and apparent activities for the CAH enzyme were calculated to be 0.51 mM and 83.59 mg/cm2, respectively; indicating CHIT-g-PANI matrix has a high affinity to immobilize CAH enzyme.http://www.expresspolymlett.com/letolt.php?file=EPL-0001053&mi=cdPolymer synthesis, molecular echitosan-graft-polyanilinecreatine amidinohydrolaseimmobilization matrix |
| spellingShingle | Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor eXPRESS Polymer Letters Polymer synthesis, molecular e chitosan-graft-polyaniline creatine amidinohydrolase immobilization matrix |
| title | Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor |
| title_full | Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor |
| title_fullStr | Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor |
| title_full_unstemmed | Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor |
| title_short | Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor |
| title_sort | chitosan g polyaniline a creatine amidinohydrolase immobilization matrix for creatine biosensor |
| topic | Polymer synthesis, molecular e chitosan-graft-polyaniline creatine amidinohydrolase immobilization matrix |
| url | http://www.expresspolymlett.com/letolt.php?file=EPL-0001053&mi=cd |