Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells.
microRNAs (miRNAs) are a large family of 21- to 22-nucleotide non-coding RNAs that interact with target mRNAs at specific sites to induce cleavage of the message or inhibit translation. miRNAs are excised in a stepwise process from primary miRNA (pri-miRNA) transcripts. The Drosha-Pasha/DGCR8 comple...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2005-07-01
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| Series: | PLoS Biology |
| Online Access: | http://europepmc.org/articles/PMC1141268?pdf=render |
| _version_ | 1818917474205171712 |
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| author | Kuniaki Saito Akira Ishizuka Haruhiko Siomi Mikiko C Siomi |
| author_facet | Kuniaki Saito Akira Ishizuka Haruhiko Siomi Mikiko C Siomi |
| author_sort | Kuniaki Saito |
| collection | DOAJ |
| description | microRNAs (miRNAs) are a large family of 21- to 22-nucleotide non-coding RNAs that interact with target mRNAs at specific sites to induce cleavage of the message or inhibit translation. miRNAs are excised in a stepwise process from primary miRNA (pri-miRNA) transcripts. The Drosha-Pasha/DGCR8 complex in the nucleus cleaves pri-miRNAs to release hairpin-shaped precursor miRNAs (pre-miRNAs). These pre-miRNAs are then exported to the cytoplasm and further processed by Dicer to mature miRNAs. Here we show that Drosophila Dicer-1 interacts with Loquacious, a double-stranded RNA-binding domain protein. Depletion of Loquacious results in pre-miRNA accumulation in Drosophila S2 cells, as is the case for depletion of Dicer-1. Immuno-affinity purification experiments revealed that along with Dicer-1, Loquacious resides in a functional pre-miRNA processing complex, and stimulates and directs the specific pre-miRNA processing activity. These results support a model in which Loquacious mediates miRNA biogenesis and, thereby, the expression of genes regulated by miRNAs. |
| first_indexed | 2024-12-20T00:34:38Z |
| format | Article |
| id | doaj.art-2da4b990f5124231b1f49a2fe4294212 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-20T00:34:38Z |
| publishDate | 2005-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-2da4b990f5124231b1f49a2fe42942122022-12-21T19:59:49ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852005-07-0137e23510.1371/journal.pbio.0030235Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells.Kuniaki SaitoAkira IshizukaHaruhiko SiomiMikiko C SiomimicroRNAs (miRNAs) are a large family of 21- to 22-nucleotide non-coding RNAs that interact with target mRNAs at specific sites to induce cleavage of the message or inhibit translation. miRNAs are excised in a stepwise process from primary miRNA (pri-miRNA) transcripts. The Drosha-Pasha/DGCR8 complex in the nucleus cleaves pri-miRNAs to release hairpin-shaped precursor miRNAs (pre-miRNAs). These pre-miRNAs are then exported to the cytoplasm and further processed by Dicer to mature miRNAs. Here we show that Drosophila Dicer-1 interacts with Loquacious, a double-stranded RNA-binding domain protein. Depletion of Loquacious results in pre-miRNA accumulation in Drosophila S2 cells, as is the case for depletion of Dicer-1. Immuno-affinity purification experiments revealed that along with Dicer-1, Loquacious resides in a functional pre-miRNA processing complex, and stimulates and directs the specific pre-miRNA processing activity. These results support a model in which Loquacious mediates miRNA biogenesis and, thereby, the expression of genes regulated by miRNAs.http://europepmc.org/articles/PMC1141268?pdf=render |
| spellingShingle | Kuniaki Saito Akira Ishizuka Haruhiko Siomi Mikiko C Siomi Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells. PLoS Biology |
| title | Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells. |
| title_full | Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells. |
| title_fullStr | Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells. |
| title_full_unstemmed | Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells. |
| title_short | Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells. |
| title_sort | processing of pre micrornas by the dicer 1 loquacious complex in drosophila cells |
| url | http://europepmc.org/articles/PMC1141268?pdf=render |
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