5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers
In order to extend the genetic data on the Sciaenidae fish family, the present study had the purpose to characterize PCR-generated 5S rDNA repeats of twelve species of this group through PAGE (Polyacrylamide Gel Electrophoresis) analysis. The results showed the occurrence of at least two different 5...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2008-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200025 |
| _version_ | 1819153600297828352 |
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| author | Fernanda A. Alves-Costa Cesar Martins Fernanda Del Campos de Matos Fausto Foresti Claudio Oliveira Adriane P. Wasko |
| author_facet | Fernanda A. Alves-Costa Cesar Martins Fernanda Del Campos de Matos Fausto Foresti Claudio Oliveira Adriane P. Wasko |
| author_sort | Fernanda A. Alves-Costa |
| collection | DOAJ |
| description | In order to extend the genetic data on the Sciaenidae fish family, the present study had the purpose to characterize PCR-generated 5S rDNA repeats of twelve species of this group through PAGE (Polyacrylamide Gel Electrophoresis) analysis. The results showed the occurrence of at least two different 5S rDNA size classes in all the species. Moreover, 5S rDNA repeats of one of the studied species - Isopisthus parvipinnis - were cloned and subjected to nucleotide sequencing and Southern blot membrane hybridization analyses, which permitted to confirm the existence of two major 5S rDNA classes. Phylogenetic analysis based on the nucleotide sequences of different 5S rDNA repeats of I. parvipinnis lead to their separation into two major clusters. These results may reflect the high dynamism that rules the evolution rate of 5S rDNA repeats. The obtained data suggest that 5S rDNA can be useful in genetic analyses to identify species-specific markers and determine relationships among species of the Sciaenidae group. |
| first_indexed | 2024-12-22T15:07:46Z |
| format | Article |
| id | doaj.art-2da5ea0f029546e1bc04da917c1f9c2c |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-22T15:07:46Z |
| publishDate | 2008-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-2da5ea0f029546e1bc04da917c1f9c2c2022-12-21T18:21:56ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852008-01-0131130330710.1590/S1415-475720080002000255S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markersFernanda A. Alves-CostaCesar MartinsFernanda Del Campos de MatosFausto ForestiClaudio OliveiraAdriane P. WaskoIn order to extend the genetic data on the Sciaenidae fish family, the present study had the purpose to characterize PCR-generated 5S rDNA repeats of twelve species of this group through PAGE (Polyacrylamide Gel Electrophoresis) analysis. The results showed the occurrence of at least two different 5S rDNA size classes in all the species. Moreover, 5S rDNA repeats of one of the studied species - Isopisthus parvipinnis - were cloned and subjected to nucleotide sequencing and Southern blot membrane hybridization analyses, which permitted to confirm the existence of two major 5S rDNA classes. Phylogenetic analysis based on the nucleotide sequences of different 5S rDNA repeats of I. parvipinnis lead to their separation into two major clusters. These results may reflect the high dynamism that rules the evolution rate of 5S rDNA repeats. The obtained data suggest that 5S rDNA can be useful in genetic analyses to identify species-specific markers and determine relationships among species of the Sciaenidae group.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200025SciaenidaefishIsopisthus parvipinnis5S rDNAmolecular markers |
| spellingShingle | Fernanda A. Alves-Costa Cesar Martins Fernanda Del Campos de Matos Fausto Foresti Claudio Oliveira Adriane P. Wasko 5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers Genetics and Molecular Biology Sciaenidae fish Isopisthus parvipinnis 5S rDNA molecular markers |
| title | 5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers |
| title_full | 5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers |
| title_fullStr | 5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers |
| title_full_unstemmed | 5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers |
| title_short | 5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers |
| title_sort | 5s rdna characterization in twelve sciaenidae fish species teleostei perciformes depicting gene diversity and molecular markers |
| topic | Sciaenidae fish Isopisthus parvipinnis 5S rDNA molecular markers |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200025 |
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