Chemical Composition of the Fixed Oil of <i>Harconia speciosa</i> and Modulation of the Antibiotic Activity against Non-Resistant and MDR Bacterial Strains
The species <i>Hancornia speciosa</i> (mangabeira) has varied potential. The bark has astringent properties, latex is used for tuberculosis, ulcers, herpes, dermatoses and warts, leaf tea is used for menstrual cramps and the root decoction to treat dislocations and hypertension. The aim...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Separations |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2297-8739/9/9/249 |
| _version_ | 1797482390872915968 |
|---|---|
| author | Erlânio O. de Sousa Maria do Socorro Costa Cícera Datiane M. Oliveira-Tintino Carla de Fátima A. Nonato Jacqueline C. A. Pinheiro Henrique Douglas M. Coutinho Irwin Rose A. de Menezes José Galberto M. Costa |
| author_facet | Erlânio O. de Sousa Maria do Socorro Costa Cícera Datiane M. Oliveira-Tintino Carla de Fátima A. Nonato Jacqueline C. A. Pinheiro Henrique Douglas M. Coutinho Irwin Rose A. de Menezes José Galberto M. Costa |
| author_sort | Erlânio O. de Sousa |
| collection | DOAJ |
| description | The species <i>Hancornia speciosa</i> (mangabeira) has varied potential. The bark has astringent properties, latex is used for tuberculosis, ulcers, herpes, dermatoses and warts, leaf tea is used for menstrual cramps and the root decoction to treat dislocations and hypertension. The aim of this work was to analyze the chemical composition of the fixed oil and antibacterial activities alone or in association with aminoglycosides against standard and MDR bacteria using broth microdilution assays. In the analysis of the oil by GC/MS, a high content of unsaturated fatty acids (73.46%) was identified in relation to saturated fatty acids (26.15%). Palmitic acid (22.49%) and elaidic acid (69.50%) were the main fatty acids identified. The antibacterial test results showed a more significant oil activity against <i>Staphylococcus aureus</i> SA–ATCC 6538 (MIC = 512 μg/mL). For other strains including standards and multidrug resistant bacteria, the oil presented MIC ≥ 1024 μg/mL. In association with antibiotics, the oil was able to improve antibacterial activity against bacterial strains. A synergic effect was observed for <i>S. aureus</i> SA–10, with MIC reduction of amikacin and gentamicin by 50.00 and 60.00%, respectively. The most significant association was found for <i>Escherichia coli</i> EC–06, with a reduction in MIC of 81.25%. The results indicate that seed oil of <i>H. speciosa</i> has the potential to act on bacterial resistance to aminoglycoside antibiotics. |
| first_indexed | 2024-03-09T22:31:39Z |
| format | Article |
| id | doaj.art-2dab63335f894735bf93bc1809d44159 |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-09T22:31:39Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-2dab63335f894735bf93bc1809d441592023-11-23T18:54:45ZengMDPI AGSeparations2297-87392022-09-019924910.3390/separations9090249Chemical Composition of the Fixed Oil of <i>Harconia speciosa</i> and Modulation of the Antibiotic Activity against Non-Resistant and MDR Bacterial StrainsErlânio O. de Sousa0Maria do Socorro Costa1Cícera Datiane M. Oliveira-Tintino2Carla de Fátima A. Nonato3Jacqueline C. A. Pinheiro4Henrique Douglas M. Coutinho5Irwin Rose A. de Menezes6José Galberto M. Costa7Laboratory Analysis Physical-Chemistry of Food, Faculty of Technology Cariri—FATEC, Juazeiro do Norte 63040-000, CE, BrazilLaboratory of Microbiology and Molecular Biology—LMBM, Regional University of Cariri—URCA, Crato 63105-000, CE, BrazilLaboratory of Microbiology and Molecular Biology—LMBM, Regional University of Cariri—URCA, Crato 63105-000, CE, BrazilLaboratory of Research in Natural Products—LPPN, Regional University of Cariri—URCA, Crato 63105-000, CE, BrazilLaboratory of Bioassays—LABIO, Federal University of Cariri—UFCA, Brejos Santos 63260-000, CE, BrazilLaboratory of Microbiology and Molecular Biology—LMBM, Regional University of Cariri—URCA, Crato 63105-000, CE, BrazilLaboratory of Pharmacology and Molecular Chemistry—LFQM, Regional University of Cariri—URCA, Crato 63105-000, CE, BrazilLaboratory of Research in Natural Products—LPPN, Regional University of Cariri—URCA, Crato 63105-000, CE, BrazilThe species <i>Hancornia speciosa</i> (mangabeira) has varied potential. The bark has astringent properties, latex is used for tuberculosis, ulcers, herpes, dermatoses and warts, leaf tea is used for menstrual cramps and the root decoction to treat dislocations and hypertension. The aim of this work was to analyze the chemical composition of the fixed oil and antibacterial activities alone or in association with aminoglycosides against standard and MDR bacteria using broth microdilution assays. In the analysis of the oil by GC/MS, a high content of unsaturated fatty acids (73.46%) was identified in relation to saturated fatty acids (26.15%). Palmitic acid (22.49%) and elaidic acid (69.50%) were the main fatty acids identified. The antibacterial test results showed a more significant oil activity against <i>Staphylococcus aureus</i> SA–ATCC 6538 (MIC = 512 μg/mL). For other strains including standards and multidrug resistant bacteria, the oil presented MIC ≥ 1024 μg/mL. In association with antibiotics, the oil was able to improve antibacterial activity against bacterial strains. A synergic effect was observed for <i>S. aureus</i> SA–10, with MIC reduction of amikacin and gentamicin by 50.00 and 60.00%, respectively. The most significant association was found for <i>Escherichia coli</i> EC–06, with a reduction in MIC of 81.25%. The results indicate that seed oil of <i>H. speciosa</i> has the potential to act on bacterial resistance to aminoglycoside antibiotics.https://www.mdpi.com/2297-8739/9/9/249mangabafatty acidantibacterialaminoglycosides |
| spellingShingle | Erlânio O. de Sousa Maria do Socorro Costa Cícera Datiane M. Oliveira-Tintino Carla de Fátima A. Nonato Jacqueline C. A. Pinheiro Henrique Douglas M. Coutinho Irwin Rose A. de Menezes José Galberto M. Costa Chemical Composition of the Fixed Oil of <i>Harconia speciosa</i> and Modulation of the Antibiotic Activity against Non-Resistant and MDR Bacterial Strains Separations mangaba fatty acid antibacterial aminoglycosides |
| title | Chemical Composition of the Fixed Oil of <i>Harconia speciosa</i> and Modulation of the Antibiotic Activity against Non-Resistant and MDR Bacterial Strains |
| title_full | Chemical Composition of the Fixed Oil of <i>Harconia speciosa</i> and Modulation of the Antibiotic Activity against Non-Resistant and MDR Bacterial Strains |
| title_fullStr | Chemical Composition of the Fixed Oil of <i>Harconia speciosa</i> and Modulation of the Antibiotic Activity against Non-Resistant and MDR Bacterial Strains |
| title_full_unstemmed | Chemical Composition of the Fixed Oil of <i>Harconia speciosa</i> and Modulation of the Antibiotic Activity against Non-Resistant and MDR Bacterial Strains |
| title_short | Chemical Composition of the Fixed Oil of <i>Harconia speciosa</i> and Modulation of the Antibiotic Activity against Non-Resistant and MDR Bacterial Strains |
| title_sort | chemical composition of the fixed oil of i harconia speciosa i and modulation of the antibiotic activity against non resistant and mdr bacterial strains |
| topic | mangaba fatty acid antibacterial aminoglycosides |
| url | https://www.mdpi.com/2297-8739/9/9/249 |
| work_keys_str_mv | AT erlanioodesousa chemicalcompositionofthefixedoilofiharconiaspeciosaiandmodulationoftheantibioticactivityagainstnonresistantandmdrbacterialstrains AT mariadosocorrocosta chemicalcompositionofthefixedoilofiharconiaspeciosaiandmodulationoftheantibioticactivityagainstnonresistantandmdrbacterialstrains AT ciceradatianemoliveiratintino chemicalcompositionofthefixedoilofiharconiaspeciosaiandmodulationoftheantibioticactivityagainstnonresistantandmdrbacterialstrains AT carladefatimaanonato chemicalcompositionofthefixedoilofiharconiaspeciosaiandmodulationoftheantibioticactivityagainstnonresistantandmdrbacterialstrains AT jacquelinecapinheiro chemicalcompositionofthefixedoilofiharconiaspeciosaiandmodulationoftheantibioticactivityagainstnonresistantandmdrbacterialstrains AT henriquedouglasmcoutinho chemicalcompositionofthefixedoilofiharconiaspeciosaiandmodulationoftheantibioticactivityagainstnonresistantandmdrbacterialstrains AT irwinroseademenezes chemicalcompositionofthefixedoilofiharconiaspeciosaiandmodulationoftheantibioticactivityagainstnonresistantandmdrbacterialstrains AT josegalbertomcosta chemicalcompositionofthefixedoilofiharconiaspeciosaiandmodulationoftheantibioticactivityagainstnonresistantandmdrbacterialstrains |