The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration
Aim: This study aims to determine the effect of seminal vesicle extract on cyclic adenosine monophosphate responsive element modulator (CREM) expression in rat Sertoli cells. Materials and Methods: This study examined the expression of CREM on 20 male rats (Rattus norvegicus) at 4 months of age, we...
| Glavni autori: | , , , , , , , |
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| Format: | Članak |
| Jezik: | English |
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Veterinary World
2016-09-01
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| Serija: | Veterinary World |
| Teme: | |
| Online pristup: | http://www.veterinaryworld.org/Vol.9/September-2016/14.pdf |
| _version_ | 1831547631773417472 |
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| author | Muslim Akmal Tongku Nizwan Siregar Sri Wahyuni Hamny Mustafa Kamal Nasution Wiwik Indriati Budianto Panjaitan Dwinna Aliza |
| author_facet | Muslim Akmal Tongku Nizwan Siregar Sri Wahyuni Hamny Mustafa Kamal Nasution Wiwik Indriati Budianto Panjaitan Dwinna Aliza |
| author_sort | Muslim Akmal |
| collection | DOAJ |
| description | Aim: This study aims to determine the effect of seminal vesicle extract on cyclic adenosine monophosphate responsive element modulator (CREM) expression in rat Sertoli cells.
Materials and Methods: This study examined the expression of CREM on 20 male rats (Rattus norvegicus) at 4 months of age, weighing 250-300 g. The rats were divided into four groups: K0, KP1, KP2, and KP3. K0 group was injected with 0.2 ml normal saline; KP1 was injected with 25 mg cloprostenol (Prostavet C, Virbac S. A); KP2 and KP3 were injected with 0.2 and 0.4 ml seminal vesicle extract, respectively. The treatments were conducted 5 times within 12-day interval. At the end of the study, the rats were euthanized by cervical dislocation; then, the testicles were necropsied and processed for histology observation using immunohistochemistry staining.
Results: CREM expression in rat Sertoli cells was not altered by the administration of either 0.2 or 0.4 ml seminal vesicle extract.
Conclusion: The administration of seminal vesicle extract is unable to increase CREM expression in rat Sertoli cells. |
| first_indexed | 2024-12-17T02:05:19Z |
| format | Article |
| id | doaj.art-2db2baed2bbf4c40a70d07eaf039e4c2 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-17T02:05:19Z |
| publishDate | 2016-09-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-2db2baed2bbf4c40a70d07eaf039e4c22022-12-21T22:07:43ZengVeterinary WorldVeterinary World0972-89882231-09162016-09-01991001100510.14202/vetworld.2016.1001-1005The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administrationMuslim Akmal0Tongku Nizwan Siregar1Sri Wahyuni2Hamny3Mustafa Kamal Nasution4Wiwik Indriati5Budianto Panjaitan6Dwinna Aliza7Laboratory of Histology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia; muslim_akmal70@yahoo.comLaboratory of Reproduction, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia; tongku_ns@yahoo.comLaboratory of Research, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia; Laboratory of Anatomy, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia; yuyun.anwar@gmail.comLaboratory of Anatomy, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia; hamny@unsyiah.ac.idDepartment of PGMI , Faculty of Tarbiyah, STAIN Gajah Putih Takengon, Aceh Tengah, Aceh, Indonesia; kamalnasution@gmail.comStudent at Veterinary Public Health Graduate Program, Syiah Kuala University, Banda Aceh, Aceh, Indonesia; indria@yahoo.co.idLaboratory of Clinic, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia; antopjt@gmail.comLaboratory of Pathology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia; dwinna2000@yahoo.comAim: This study aims to determine the effect of seminal vesicle extract on cyclic adenosine monophosphate responsive element modulator (CREM) expression in rat Sertoli cells. Materials and Methods: This study examined the expression of CREM on 20 male rats (Rattus norvegicus) at 4 months of age, weighing 250-300 g. The rats were divided into four groups: K0, KP1, KP2, and KP3. K0 group was injected with 0.2 ml normal saline; KP1 was injected with 25 mg cloprostenol (Prostavet C, Virbac S. A); KP2 and KP3 were injected with 0.2 and 0.4 ml seminal vesicle extract, respectively. The treatments were conducted 5 times within 12-day interval. At the end of the study, the rats were euthanized by cervical dislocation; then, the testicles were necropsied and processed for histology observation using immunohistochemistry staining. Results: CREM expression in rat Sertoli cells was not altered by the administration of either 0.2 or 0.4 ml seminal vesicle extract. Conclusion: The administration of seminal vesicle extract is unable to increase CREM expression in rat Sertoli cells.http://www.veterinaryworld.org/Vol.9/September-2016/14.pdfcyclic adenosine monophosphate responsive element modulatorseminal vesicle extractSertoli cellsspermatogenesis |
| spellingShingle | Muslim Akmal Tongku Nizwan Siregar Sri Wahyuni Hamny Mustafa Kamal Nasution Wiwik Indriati Budianto Panjaitan Dwinna Aliza The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration Veterinary World cyclic adenosine monophosphate responsive element modulator seminal vesicle extract Sertoli cells spermatogenesis |
| title | The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration |
| title_full | The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration |
| title_fullStr | The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration |
| title_full_unstemmed | The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration |
| title_short | The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration |
| title_sort | expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration |
| topic | cyclic adenosine monophosphate responsive element modulator seminal vesicle extract Sertoli cells spermatogenesis |
| url | http://www.veterinaryworld.org/Vol.9/September-2016/14.pdf |
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