High pressure pasteurization: Simultaneous native tissue decellularization and sterilization
Introduction: Terminal sterilization is important for the clinical applicability of decellularized xenografts. High hydrostatic pressurization (HHP) process is a potential strategy for decellularization and decontamination of xenografts; however, its disinfection efficiency remains poorly elucidated...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Regenerative Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320424000129 |
| _version_ | 1797319401004859392 |
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| author | Akihisa Otaka Takashi Yamamoto Tetsuji Yamaoka |
| author_facet | Akihisa Otaka Takashi Yamamoto Tetsuji Yamaoka |
| author_sort | Akihisa Otaka |
| collection | DOAJ |
| description | Introduction: Terminal sterilization is important for the clinical applicability of decellularized xenografts. High hydrostatic pressurization (HHP) process is a potential strategy for decellularization and decontamination of xenografts; however, its disinfection efficiency remains poorly elucidated. This study investigated the disinfection efficacy of the HHP process at physiologically relevant 36 °C against difficult-to-kill spore-forming bacteria. Methods: Bacillus atrophaeus and Geobacillus stearothermophilus were suspended in a pressurization medium with or without antibiotic agents and pressurized under two different HHP procedures: repeated and sustained pressurization. Results: The sustained pressurizing conditions, exploited for the conventional tissue decellularization, did not effectively eliminate the bacteria; however, repeated pressurization greatly increased the disinfection effect. Moreover, the antibiotic-containing pressurization medium further increased the disinfection efficiency to the level required for sterilization. Conclusions: The optimized high hydrostatic pressurization can be used to sterilize biological tissues during the decellularization process and is a promising strategy for manufacturing tissue-derived healthcare products. |
| first_indexed | 2024-03-08T04:07:20Z |
| format | Article |
| id | doaj.art-2db4992a80c845bab001019418750d62 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-03-08T04:07:20Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-2db4992a80c845bab001019418750d622024-02-09T04:48:28ZengElsevierRegenerative Therapy2352-32042024-06-012628High pressure pasteurization: Simultaneous native tissue decellularization and sterilizationAkihisa Otaka0Takashi Yamamoto1Tetsuji Yamaoka2Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, 6-1 Kishibe-Shimmachi, Suita, Osaka, 564-8565, JapanResearch & Development, JMS Co. Ltd, 12-17 Kako-machi, Naka-ku, Hiroshima, JapanDepartment of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, 6-1 Kishibe-Shimmachi, Suita, Osaka, 564-8565, Japan; Corresponding author.Introduction: Terminal sterilization is important for the clinical applicability of decellularized xenografts. High hydrostatic pressurization (HHP) process is a potential strategy for decellularization and decontamination of xenografts; however, its disinfection efficiency remains poorly elucidated. This study investigated the disinfection efficacy of the HHP process at physiologically relevant 36 °C against difficult-to-kill spore-forming bacteria. Methods: Bacillus atrophaeus and Geobacillus stearothermophilus were suspended in a pressurization medium with or without antibiotic agents and pressurized under two different HHP procedures: repeated and sustained pressurization. Results: The sustained pressurizing conditions, exploited for the conventional tissue decellularization, did not effectively eliminate the bacteria; however, repeated pressurization greatly increased the disinfection effect. Moreover, the antibiotic-containing pressurization medium further increased the disinfection efficiency to the level required for sterilization. Conclusions: The optimized high hydrostatic pressurization can be used to sterilize biological tissues during the decellularization process and is a promising strategy for manufacturing tissue-derived healthcare products.http://www.sciencedirect.com/science/article/pii/S2352320424000129BiomaterialsXenograftsDecellularizationHigh hydrostatic pressureSterilization |
| spellingShingle | Akihisa Otaka Takashi Yamamoto Tetsuji Yamaoka High pressure pasteurization: Simultaneous native tissue decellularization and sterilization Regenerative Therapy Biomaterials Xenografts Decellularization High hydrostatic pressure Sterilization |
| title | High pressure pasteurization: Simultaneous native tissue decellularization and sterilization |
| title_full | High pressure pasteurization: Simultaneous native tissue decellularization and sterilization |
| title_fullStr | High pressure pasteurization: Simultaneous native tissue decellularization and sterilization |
| title_full_unstemmed | High pressure pasteurization: Simultaneous native tissue decellularization and sterilization |
| title_short | High pressure pasteurization: Simultaneous native tissue decellularization and sterilization |
| title_sort | high pressure pasteurization simultaneous native tissue decellularization and sterilization |
| topic | Biomaterials Xenografts Decellularization High hydrostatic pressure Sterilization |
| url | http://www.sciencedirect.com/science/article/pii/S2352320424000129 |
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